LOCUS       sequence-251740-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Low level expression of E.coli G.stearothermophillus MutY chimera 
            protein with deletion of residues FSH 307-309 in Gs MutY..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Russelburg LP, O'Shea Murray VL, Demir M, Knutsen KR, Sehgal SL, Cao
            S, David SS, Horvath MP
  TITLE     Structural Basis for Finding OG Lesions and Avoiding Undamaged G by 
            the DNA Glycosylase MutY.
  JOURNAL   ACS Chem Biol. 2019 Dec 27. doi: 10.1021/acschembio.9b00639.
  PUBMED    31829624
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2019 Dec 27. doi: 10.1021/acschembio.9b00639."
COMMENT     Sequence Label: EcNGsC-DelFSH(307-309)-pKK223
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..34
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     23..45
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcgtataatg tgtggaattg tgagcggata acaatttcac acaggaaaca gaattcggtg
       61 accatgcaag cgtcgcaatt ttcagcccag gttctggact ggtacgataa atacgggcga
      121 aaaactctgc cctggcaaat tgacaagacg ccctacaaag tatggctctc agaagtgatg
      181 ttgcaacaaa ctcaggttgc gaccgttatc ccctattttg aacgctttat ggcgcgcttc
      241 ccgacggtga ccgatctcgc caatgcgccg ctcgacgaag ttctccactt gtggaccggg
      301 cttggctatt acgcccgcgc gcgcaatctg cataaagcgg cacaacaagt ggcgacctta
      361 cacggcggta aattcccgga aacctttgag gaagttgcag cactgccggg cgtcgggcgt
      421 tccaccgcag gcgcgattct ctcgctttct ctgggtaagc actttccgat tctcgacggt
      481 aacgtcaaac gcgtgctggc gcgctgctat gctgtaagcg gctggcctgg gaaaaaagag
      541 gtcgagaata aattatggag tttgagcgag caggtgacgc ccgcggttgg cgtggaacgg
      601 tttaatcagg cgatgatgga tttgggtgcg atgatttgta cgcgctcgaa accgaaatgt
      661 tcgctctgtc cgctacaaaa cggatgtatt gccgccgcca acaatagctg ggcgcttt
//
