LOCUS       sequence-251743-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Low level expression of E.coli G.stearothermophillus MutY chimera 
            protein with amino acid changes S308P and H309D in Gs MutY..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Russelburg LP, O'Shea Murray VL, Demir M, Knutsen KR, Sehgal SL, Cao
            S, David SS, Horvath MP
  TITLE     Structural Basis for Finding OG Lesions and Avoiding Undamaged G by 
            the DNA Glycosylase MutY.
  JOURNAL   ACS Chem Biol. 2019 Dec 27. doi: 10.1021/acschembio.9b00639.
  PUBMED    31829624
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2019 Dec 27. doi: 10.1021/acschembio.9b00639."
COMMENT     Sequence Label: EcNGsC-S308P-H309D-pKK223
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..37
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     26..48
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagaattcg
       61 gtgaccatgc aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg
      121 cgaaaaactc tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg
      181 atgttgcaac aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggcgcgc
      241 ttcccgacgg tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc
      301 gggcttggct attacgcccg cgcgcgcaat ctgcataaag cggcacaaca agtggcgacc
      361 ttacacggcg gtaaattccc ggaaaccttt gaggaagttg cagcactgcc gggcgtcggg
      421 cgttccaccg caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac
      481 ggtaacgtca aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc tgggaaaaaa
      541 gaggtcgaga ataaattatg gagtttgagc gagcaggtga cgcccgcggt tggcgtggaa
      601 cggtttaatc aggcgatgat ggatttgggt gcgatgattt gtacgcgctc gaaaccgaaa
      661 tgttcgctct gtccgctaca aaacggatgt attgccgccg ccaacaatag ctg
//
