LOCUS       sequence-251745-        6209 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Xylanase C+GUS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Alcocer MJ, Furniss CS, Kroon PA, Campbell M, Archer DB
  TITLE     Comparison of modular and non-modular xylanases as carrier proteins 
            for the efficient secretion of heterologous proteins from 
            Penicillium funiculosum.
  JOURNAL   Appl Microbiol Biotechnol. 2003 Feb;60(6):726-32. doi: 
            10.1007/s00253-002-1184-4. Epub 2002 Dec 21.
  PUBMED    12664153
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-002-1184-4"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2003-02"; volume: "60"; issue: "6"; pages: 
            "726-32"
COMMENT     Sequence Label: pMJA028
FEATURES             Location/Qualifiers
     source          1..6209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(126..194,195..986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(344..363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1646..1665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1899..1916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2069..2074,2075..2092,2093..2099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2107..2123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2112..2134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2131..2147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2131..2147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2166..2186
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2168..2186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3419..5230
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     primer_bind     complement(5561..5580)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5562..5580)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5590..5607)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5590..5606)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5599..5621)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5747..6203)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5885..5906)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6097..6116
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
     2221 gctctagaac tagtgagtat gtaagcgagt ctgatcttcc cgcccgccaa tcaaatcgca
     2281 ggatcacaga atgcaaatac cttctgatcc caaccacaac cagtatttct gattcgaatc
     2341 aaaatcgtgg catgtaattc gcgggatcag aatgcggttt ccaccggcca aaatgaccga
     2401 tctgcgttgc cacggctggt aatggcagga tcagagcagc agcgttgaat cagtcagatc
     2461 aagcgccgct cccgctgatt ggccagatca cccactagcg cgtgatccca ggcaccttcc
     2521 cgcgtccggc tctttctata gaaatatctc ccgccgcgtc ttccttccca ccttctttct
     2581 cctctccatc tccagctcca tcttcaagct tactcaacat tatcaacgcg tttacgcatc
     2641 cacattcaat tacttatcat ctcatcccca tacagaattc aaaatgaagc tcttcctagc
     2701 tgcaattgtc ctttgcgcaa ctgccgcgac agccttccca tcagaacttg ctcaacgcgc
     2761 tgcgggagac cttagcaagc gtcaatcaat cacgaccagc cagactggga cgaacaacgg
     2821 ctactactac tcgttctgga ccaacggcgg aggagaagtc acttacacaa atggcgacaa
     2881 tggcgagtac agcgtgacat gggtcgattg tggtgacttt acatctggca agggctggaa
     2941 tccagccaat gcacagtaag tttccctctt tccttctaag cttatattgt acgtactcac
     3001 aatttgcagg actgtcacgt actcaggaga atttaacccc tctggaaacg cttatttggc
     3061 tgtctacggg tggacaacag atcctcttgt cgaatactac atcctggagt cctacggcac
     3121 ctataaccca tcatctggac ttacttcact tggccaggtc actagcgatg gtggcaccta
     3181 cgatatctac tcaacccagc gtgtcaacca gccttccatt gagggaactt ccaccttcaa
     3241 ccagtactgg tcagttcgca ccgagaagcg agtcggcgga actgtcacca cggccaacca
     3301 ctttgcagca tggaaggcac ttggacttga aatgggcact tataactata tgattgtgtc
     3361 caccgaaggc tacgagagca gtggctctag taccatcaca gtgtccatcg ataagcgtat
     3421 gttacgtcct gtagaaaccc caacccgtga aatcaaaaaa ctcgacggcc tgtgggcatt
     3481 cagtctggat cgcgaaaact gtggaattga tcagcgttgg tgggaaagcg cgttacaaga
     3541 aagccgggca attgctgtgc caggcagttt taacgatcag ttcgccgatg cagatattcg
     3601 taattatgcg ggcaacgtct ggtatcagcg cgaagtcttt ataccgaaag gttgggcagg
     3661 ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac ggcaaagtgt gggtcaataa
     3721 tcaggaagtg atggagcatc agggcggcta tacgccattt gaagccgatg tcacgccgta
     3781 tgttattgcc gggaaaagtg tacgtatcac cgtttgtgtg aacaacgaac tgaactggca
     3841 gactatcccg ccgggaatgg tgattaccga cgaaaacggc aagaaaaagc agtcttactt
     3901 ccatgatttc tttaactatg ccggaatcca tcgcagcgta atgctctaca ccacgccgaa
     3961 cacctgggtg gacgatatca ccgtggtgac gcatgtcgcg caagactgta accacgcgtc
     4021 tgttgactgg caggtggtgg ccaatggtga tgtcagcgtt gaactgcgtg atgcggatca
     4081 acaggtggtt gcaactggac aaggcactag cgggactttg caagtggtga atccgcacct
     4141 ctggcaaccg ggtgaaggtt atctctatga actgtgcgtc acagccaaaa gccagacaga
     4201 gtgtgatatc tacccgcttc gcgtcggcat ccggtcagtg gcagtgaagg gcgaacagtt
     4261 cctgattaac cacaaaccgt tctactttac tggctttggt cgtcatgaag atgcggactt
     4321 gcgtggcaaa ggattcgata acgtgctgat ggtgcacgac cacgcattaa tggactggat
     4381 tggggccaac tcctaccgta cctcgcatta cccttacgct gaagagatgc tcgactgggc
     4441 agatgaacat ggcatcgtgg tgattgatga aactgctgct gtcggcttta acctctcttt
     4501 aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg tacagcgaag aggcagtcaa
     4561 cggggaaact cagcaagcgc acttacaggc gattaaagag ctgatagcgc gtgacaaaaa
     4621 ccacccaagc gtggtgatgt ggagtattgc caacgaaccg gatacccgtc cgcaaggtgc
     4681 acgggaatat ttcgcgccac tggcggaagc aacgcgtaaa ctcgacccga cgcgtccgat
     4741 cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat accatcagcg atctctttga
     4801 tgtgctgtgc ctgaaccgtt attacggatg gtatgtccaa agcggcgatt tggaaacggc
     4861 agagaaggta ctggaaaaag aacttctggc ctggcaggag aaactgcatc agccgattat
     4921 catcaccgaa tacggcgtgg atacgttagc cgggctgcac tcaatgtaca ccgacatgtg
     4981 gagtgaagag tatcagtgtg catggctgga tatgtatcac cgcgtctttg atcgcgtcag
     5041 cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt gcgacctcgc aaggcatatt
     5101 gcgcgttggc ggtaacaaga aagggatctt cactcgcgac cgcaaaccga agtcggcggc
     5161 ttttctgctg caaaaacgct ggactggcat gaacttcggt gaaaaaccgc agcagggagg
     5221 caaacaatga ctcgagacgt tttctcttca ttgttttgtc gcggcctcgc caacgcggtc
     5281 gacgcgtctg ctcgccgctg tggtgacgcg ttgctcctta ttctgggttg ggtttcggta
     5341 tgacagggtc gtcagggtgt tctctgaata gtttttcttg ttttcttcac gtctttatgg
     5401 gtctgggtct tgcatctttt tactactact taaattatac ctcgtctaca ctggtcggct
     5461 tgtggcctat gggtccgaat atctttgtat catagcggcc gtttatcctg gtggactgga
     5521 gttgcgactg ctcgtcatcg cacatgaggt acccaattcg ccctatagtg agtcgtatta
     5581 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     5641 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     5701 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt
     5761 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt ttaaccaata
     5821 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     5881 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     5941 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     6001 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     6061 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     6121 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     6181 taatgcgccg ctacagggcg cgtcaggtg
//
