LOCUS       sequence-251748-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Low level expression of E.coli G.stearothermophillus MutY chimera 
            protein with amino acid change F307Y in Gs MutY..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Russelburg LP, O'Shea Murray VL, Demir M, Knutsen KR, Sehgal SL, Cao
            S, David SS, Horvath MP
  TITLE     Structural Basis for Finding OG Lesions and Avoiding Undamaged G by 
            the DNA Glycosylase MutY.
  JOURNAL   ACS Chem Biol. 2019 Dec 27. doi: 10.1021/acschembio.9b00639.
  PUBMED    31829624
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2019 Dec 27. doi: 10.1021/acschembio.9b00639."
COMMENT     Sequence Label: EcNGsC-F307Y-pKK223
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..43
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     32..54
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
       61 aattcggtga ccatgcaagc gtcgcaattt tcagcccagg ttctggactg gtacgataaa
      121 tacgggcgaa aaactctgcc ctggcaaatt gacaagacgc cctacaaagt atggctctca
      181 gaagtgatgt tgcaacaaac tcaggttgcg accgttatcc cctattttga acgctttatg
      241 gcgcgcttcc cgacggtgac cgatctcgcc aatgcgccgc tcgacgaagt tctccacttg
      301 tggaccgggc ttggctatta cgcccgcgcg cgcaatctgc ataaagcggc acaacaagtg
      361 gcgaccttac acggcggtaa attcccggaa acctttgagg aagttgcagc actgccgggc
      421 gtcgggcgtt ccaccgcagg cgcgattctc tcgctttctc tgggtaagca ctttccgatt
      481 ctcgacggta acgtcaaacg cgtgctggcg cgctgctatg ctgtaagcgg ctggcctggg
      541 aaaaaagagg tcgagaataa attatggagt ttgagcgagc aggtgacgcc cgcggttggc
      601 gtggaacggt ttaatcaggc gatgatggat ttgggtgcga tgatttgtac gcgctcgaaa
      661 ccgaaatgtt cgctctgtcc gctacaaaac ggatgtattg ccgccgccaa caatagctgg
      721 gcgctttatc cgggcaaaaa accg
//
