LOCUS       sequence-25175-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Horbach R, Graf A, Weihmann F, Antelo L, Mathea S, Liermann JC, 
            Opatz T, Thines E, Aguirre J, Deising HB
  TITLE     Sfp-type 4'-phosphopantetheinyl transferase is indispensable for 
            fungal pathogenicity.
  JOURNAL   Plant Cell. 2009 Oct;21(10):3379-96. doi: 10.1105/tpc.108.064188. 
            Epub 2009 Oct 30.
  PUBMED    19880801
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.108.064188"; journalName: "Plant Cell"; date: 
            "2009-10"; volume: "21"; issue: "10"; pages: "3379-96"
COMMENT     Sequence Label: pAG300
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(187..291)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     359..377
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(563..582)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     gap             639..648
                     /estimated_length=10
ORIGIN
        1 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
       61 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
      121 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
      181 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
      241 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
      301 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
      361 taggcgtatc acgaggccct ttcgtcttca agaattccac ggactataga ctatactagt
      421 atactccgtc tactgtacga tacacttccg ctcaggtcct tgtcctttaa cgaggacgcg
      481 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      541 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      601 gccggctttc cccgtcaagc tctaaatcgg gggctcccnn nnnnnnnntc cganttagtg
      661 ctttacggca cctcgacccc naaaaanttg attangggnn gangggtt
//
