LOCUS       sequence-25177-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.66
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..172)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(522..617)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(654..673)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(655..673)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(678..694)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(678..694)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     gap             797..816
                     /estimated_length=20
ORIGIN
        1 tctngagctc gnttctacag gtcactaata ccatctaagt agttggttca tagtgactgc
       61 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      121 ttgatattta tatcatttta cgtttctcgt tcaactttct tgtacaaagt gggatagttt
      181 ctctcgtcga aatttctgat cttgactaac atgtactttc tattttttac ttatggatta
      241 tataatcaag tccaataacc tggtgcagtc ccaaggacat catcaataat tttccccatt
      301 tttgtcgttc tttttcttct cgagtccgac cccaaaatgt tgtattcaca ggctttcaaa
      361 actcaccccc acattcccac atttgtatct agtctgtata ttattgttat cttgaatttt
      421 accaatgttt cgttctcaat tgcatagccc tcgttatgtg gactcataaa atgaaatttc
      481 tcgtaatcag catacaattg tttcaagttt tacggtgcct ccaactttat tatacaaagt
      541 tggcattata aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
      601 aaataaaatc attatttgga gctccatggt agcgttaacg cggccgcgat atcccctata
      661 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaantct
      721 ctgatgttac attgcacaag ataaaatata tcatcatgac nnaaaactgt ctgctnacat
      781 aaacagtant acanggnnnn nnnnnnnnnn nnnnnntnna nng
//
