LOCUS       sequence-25179-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.64
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(50..173)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(515..610)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(647..666)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(648..666)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(671..687)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(671..687)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     gap             761..778
                     /estimated_length=18
     gap             780..792
                     /estimated_length=13
ORIGIN
        1 tctngagctc ganttctaca ggtcactaat accatctaag tagttggttc atagtgactg
       61 catatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
      121 attgatattt atatcatttt acgtttctcg ttcaactttc ttgtacaaag tgggataatg
      181 taattttgtg tatgtacccc ttactgccac aataccccag attttgaaca tattttatcg
      241 cacccccata ctcgcccatt gtactattaa ttgtttttta attgatacct actcgcctct
      301 tttgtaagtc gctagattgt gcctctacta gaaacgccat ctagtgataa taaccgacgc
      361 ttcgcttctt gttgcatatg tagttgaccc catcgcgtac ttttgtacat tttgtgctta
      421 tataactttt gtaacataat ggaagtaatt tttaaaggtt gctatggtgt cgtaataaag
      481 acaattgtag cttgaatcgt gtttctatac ttttcaactt tattatacaa agttggcatt
      541 ataaaaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
      601 atcattattt ggagctccat ggtagcgtta acgcggccgc gatatcccct atagtgagtc
      661 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
      721 ttacattgca caagataaaa atatatcatc atgaacanaa nnnnnnnnnn nnnnnnnnan
      781 nnnnnnnnnn nngnnnntta nnaannnnnn nnaannggaa annnncnang
//
