LOCUS       sequence-25180-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.63
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..172)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(302..397)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(434..453)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(435..453)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(458..474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(458..474)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(576..595)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(654..673)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tctngagctc gnttctacag gtcactaata ccatctaagt agttggttca tagtgactgc
       61 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      121 ttgatattta tatcatttta cgtttctcgt tcaactttct tgtacaaagt gggatgaagc
      181 aatcggagtt tttgttttaa gcggaaaata ttaattatgt atccattgtg tttattatga
      241 cttcgaactg aaataaatcg agaaattgga acttttgtga tttatttgct attcaattct
      301 gcaactttat tatacaaagt tggcattata aaaaagcatt gcttatcaat ttgttgcaac
      361 gaacaggtca ctatcagtca aaataaaatc attatttgga gctccatggt agcgttaacg
      421 cggccgcgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
      481 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
      541 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
      601 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
      661 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
      721 nnatgcgcca ganttgtttc tgannatggn nannnagcgt ngccnngatg ntacnnanna
      781 gatngncnga nnangacnng nnnnnnnnan ntnnnngccn nnnncnnnnc nnnaagnnnt
//
