LOCUS       sequence-251801-       11315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suicide vector for allelic replacement in Bacteroides, inulin 
            selection and aTC-inducible ss-Bfe1 counterselection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Garcia-Bayona L, Comstock LE
  TITLE     Streamlined Genetic Manipulation of Diverse Bacteroides and 
            Parabacteroides Isolates from the Human Gut Microbiota.
  JOURNAL   mBio. 2019 Aug 13;10(4). pii: mBio.01762-19. doi: 
            10.1128/mBio.01762-19.
  PUBMED    31409684
REFERENCE   2  (bases 1 to 11315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2019-08-13"; volume: "10(4). pii: mBio.01762-19. doi"; pages:
            " 10.1128/mBio.01762-19"
COMMENT     Sequence Label: pLGB29
FEATURES             Location/Qualifiers
     source          1..11315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(30..821,822..890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     653..672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    9377..9395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9439..9457
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             9728..10351
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9801..9820)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(10497..10513)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10497..10513)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     rep_origin      10546..10934
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            11105..11214
                     /note="incP origin of transfer"
ORIGIN
        1 acgcgttatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
       61 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
      601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
      661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
      721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
      781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      901 tttcaatcat ggccgcggga ttaaaagtcg gggattggtg aacaaaaagg tgtttctctc
      961 tttaagagaa atatcgtttt gctaaacagt tgatattgag gtatcatttt atcgtaaaag
     1021 acatttttgc tcaacaattg cttgacggaa atcaacaaat tttagcattt tgtaaaaaag
     1081 tcgctatata atttggtgaa ttggagttat tttcatattt ttgcatcccg aagagtttct
     1141 cttaaagagt gatctggaag aagcaatgaa agctgctgtt aagtctccga atcaggtatt
     1201 gttcctgaca ggtgtattcc catccggtaa acgcggatac tttgcagttg atctgactca
     1261 ggaataaatt ataaattaag gtaagaagat tgtaggataa gctaatgaaa tagaaaaagg
     1321 atgccgtcac acaacttgtc ggcattcttt tttgttttat tagttgaaaa tatagtgaaa
     1381 aagttgccta aatatgtatg ttaacaaatt atttgtcgta actttgcact ccaaatctgt
     1441 ttttaacata tggcactaat gcttagtatt atgaagaaca aaaaacttct ttgtagcgtt
     1501 tgtttcctgt ttactttcat gtcagtcctt tgggggcaaa acataacagt gaaaggaaac
     1561 gttacttcaa agactgatgg tcaaccaatt attggtgctt ccgtagtgca aaatgattct
     1621 aaatcaactg gaactatcac agatttggat ggtaacttta ctcttagtgt accaacgaat
     1681 gcattattgg caatttctta tataggatat aaagaagtaa ttattgccgc taaaccttca
     1741 ttgaagatag tgatggaaga ggatgctaag atgattgacg aagtggttgt gacaggttat
     1801 atggctgaaa agaaagccag tctgacaggt tcggtagctg tagtgaagat gaaagaggtg
     1861 gctgacatac ctactggtaa tgtgatgtca ggtttgcagg gacgtgtagc cggtatgaac
     1921 gtaacaactg atggtaaacc cggaggtggt aacacagata ctaagttacg tggtattact
     1981 accatcaaca attcttctcc tctttatgta atagacggtg ttcagacaca tgataatgtg
     2041 gcttctatta tctcatccaa tgatgttgaa tccattcagg tgttgaaaga tgcagcttcg
     2101 gctgccattt atggtgcgca agctgccaat ggtgtcatta ttatcaccac aaaacgtgct
     2161 aaggaaggcg atgtaaaagt aagttttgat atgtcgctca ctgcccagac ttttactggt
     2221 ggtattgata tgcttgacgc ttaccaatgg ggtgatgtgt attggcaggc ttataaaaat
     2281 acttttggaa ccaatcccaa tagtgtagta tatggaaatg gagaaaaggc ccaactgcaa
     2341 gaatattatt ttgaccagaa tggagtgaaa ataaagtcgg gcaacactga ttgggcaaag
     2401 gaaatatttg gtacggcttt aatgcaaaat tataatcttt ctctttctaa agggtttaag
     2461 gatgggaatg tggctcttac cttaaattat atgaatcagg atggtctttg ccgtaataca
     2521 gactacgaac gttttaattc gcgtttagcc tctaattttc gctttgtaga tggtaaagtg
     2581 cgtgtgggtg aaagtatttc ggtgaatcac tggttggagc atcttaatcc tgccggtata
     2641 gaagaattgg ttattgccca gcatccggcc attcctgtgt atgatgaaaa tggagggtat
     2701 gccggtggct atgtggatat attgggggat aagccgaatg ccattcgctt gacagacaat
     2761 gaagccaata atcgtcacaa aagttggcgt atctttggca atgtttattt ggaagtggaa
     2821 cccattaaaa atctagtgtt gaagagtcaa tttggtttga actatactac cggtttcaac
     2881 tcaactttcg tccccaaatg gagtgaagcc gatcgtaagg tagatgtaaa tgaactgacc
     2941 gtacgacaag ataatagcgt gcaatgggta tggtcgaaca ctgccaatta tagtatagac
     3001 tttgggaaga acaatatcag tgcagtgata ggaactgaag ctaagaaaga gaatggtgaa
     3061 catttacagg gatatggtcg cggattggtg atagaagatc tcgattatcg ttacttagac
     3121 actgtaactg aaggaaaaag tgtgagtaac aatgcttcta cctatgctat ggtctcttac
     3181 tttggtaagg tgaactatgc gtttgatgat aaatacctta tatcaggaac agtgcgtcgt
     3241 gatgcttctt cacgtttcgg taaagggaat aattctgcta tctttccttc agtatctgcc
     3301 ggttggagag tatcgcgtga aaaatttatg gaatgtacgc aaaactggct ttcagatctt
     3361 aaattgcgtg catcgtgggg gatcaatggt aatgatcaga ttgataacaa tgctacttac
     3421 aacctctatt acactaacat gtccaatggc tcatacaatt tcaatgggga tggtacaacg
     3481 gtattgcccg gagtacagaa agaccgttcg ggtaacaacg acctgaaatg ggaagagacc
     3541 aaacaactga actttggtat tgatgctgct ttctttgaca accgtttggg actgacactg
     3601 gattattttc aaaaaaagac tactggtatg ttaatccaga aaccttatat aggagtgatc
     3661 ggtgaaggtg gatataaatg gtacaatgct gccagtatgg ataatagcgg tgtggaagca
     3721 acctttactt ggcgagatga aataaaggat ttcaagtatg atatttcgtt caacctttcc
     3781 tattataaaa atgaaattac cgaacttccg gatgtaatca aatatacttg gggaggtggt
     3841 aatggtgtgg acaaaactat tgtaggtcaa ccttacggat catggatgtc ctataaagct
     3901 gatggtgtat tcaaaacaaa acaggaggta tatgaatacc taagcaaata tgatgtacag
     3961 attggtgctc ccggcgtagg acgtattcgc tacaaagacc ttaacggtga taacattatc
     4021 aatactgctg atatggattg gcaagggagt gaccaaccta gatttattgg tggattgaac
     4081 atcggtgcat cttacaaagg atttgacctt tctgtattct tcaatggtat gattcgcgat
     4141 gcatggaaca attccaagtt ttataccgac ctttttcaag gttggacagg taatcactct
     4201 gtacgtttga tggatgcaat gcatgcttgg aatgcttatg agcaaacggg agtttataat
     4261 tgtgatatcc ctgcattgac tgtagttgat aataatgtag aaacacgcag ttccactttc
     4321 tttattgaag atggttctta tgtgaagttg aaaactctaa ctgtgggtta tactttcccg
     4381 gataagtgga ttaagaaagc gcatctttcc aatttgcgtt tgtatctgca agcacagaac
     4441 gtttttacgc tgactaatta tacgggtgcc gacccggaag gattgggcta tccatatccc
     4501 caacctcgta cctatacatt cggtctatca cttggttttt aatttttatc ctaaatacag
     4561 aacattatga aattgaaata tatctttgca gtaggtgtga tggcactatt ggcaagttgc
     4621 gatgagagtc gttttctaga tattaaacct cagggtacac tcaatgacga tttgttatca
     4681 tcggccgatg gtgtagagct tttggttact tcagcttatg caggattgaa aggtcctaat
     4741 cgtgatatgc actgggtgcc gatgaccaat tggacgtatg gtgaagtacg ttcggataat
     4801 gcctacaaag gtggtggcgg tgtaaatgat ggagactttt gtacgctgct tgaaaccttt
     4861 aaaatagatg ctacatgggg caatgccgac gaaaaatggt ttcaacttta ttgttgcttg
     4921 caacgttgta acagcgcatt gcgggtaatg aatacgcttg atgagaacgc tttgccgatg
     4981 attaaaagtc gtaaagccga aatgaaagtg attcgtgcac acttcttctt tgaactggtt
     5041 cgccttttca agcaagtacc ttattttgat gagaatgtgg aaaatgatga ctataagtac
     5101 attcccaaca atcagtttac ccgtgaagaa cttctgggta aaatagcaca agaatttctt
     5161 gatgctgcta acgatttgcc tgatactcaa tctcaagtag ggcgtgtcac taagaacatt
     5221 gcttatgctt atgctgccaa agctagactt tatcaggctt accgacaaga tgataataac
     5281 caagtggttg ctattgataa acaattgtta gctgaagtgg tgactctttg cgacaaagta
     5341 ggtgcgcaag gaaaggcata tactcttttg aacgattttc agaaacttga tcaattggaa
     5401 tatgaaaatg gggtagagtc tgtgtttgct gtacaatatt cgatggatga tgaaaccgat
     5461 ggtgccggga atataaactg gagtaatctg ttgaatgctc ctaaaggtcc ttatggtggc
     5521 gacggatttt tcttacccag tcagaacttg atcaatgcct ataagacaga taactatgga
     5581 ttgcctttgt ttgatacttt caacgccgat gactatggaa tatacgaagg aagtgagctc
     5641 accaacattg atgcaactgt ggatcctcgt ttggatttcg ttaccggtcg tcctggtatc
     5701 acttggaaaa catataccgt tgagccttgt aaagcaaact ggatacgtaa cagcggtgaa
     5761 tatggcttta attgtacaaa gcgcttctac atctcgcctg aaagtgaaga tatgttccaa
     5821 ggttggcctt ggggagcttc tcatctcaac tggcagataa ttcgctatgc tgatgtgttg
     5881 ttatggaagg ctgaagcctt gatagagatt ggcgaagcta ccggactgga agaggctcgc
     5941 aaaattatta accgtattcg tctgcgtgcc aaaaatagtc cttatgtaaa agactttgaa
     6001 cattccgaac aggatgcagc taattatctg ataggcgaat atcctgctga gggctggagt
     6061 caggactttg cccgcaaggc attacgttgg gaacgccgta tggagtttgc tatggaaggt
     6121 gaccgtttct tcgacttggt acgttggggg attgctgcgg aaaccatgaa cagctacatt
     6181 agtgtggagc agaataagcg cgtttattat cgtggtgctc gctttgttcg tggcagagat
     6241 gaatatttgc ctattcccaa tgcacaatat aacttctctg aaggcaatta tgtgcagaat
     6301 cccggatacg gtaagtttaa ctagctctaa taccatctga catgaatgta ttattctcgt
     6361 ttaaacaagt acagaccttt ttgactatcc tggttatgat gattttctca tttttggatg
     6421 cttcggccga tacttcttcc ttattaataa aagatttggg tgaaggacat tgcctggtac
     6481 agataaatac aaatcaacgt tatttgcttt tacctgtaga ggaggtaatg cctgatgtac
     6541 gtgtcagcat gatcgttaat aataaagagg tgaatgttgc ggatgttcgt ctggcagtga
     6601 acagagtgga ctattttgtt ccgctggatc tttcggctta tgctggtaaa aacatactgt
     6661 tgaaatttaa attaggctcg aatgatccta tacgggggaa actttctgct gtttgttgca
     6721 aggaaatgaa attgtccgac acgttcgata cgggcaaccg tgaaaagttc cgtcctactt
     6781 atcatttctc acctctttat gggtggatga atgatcctaa tggaatggta tataaagacg
     6841 gtgaatacca ccttttctat caatacaatc cttatggttc caaatgggga aatatgagtt
     6901 ggggacatgc tatcagccaa gatcttgtaa actggaaaca tcttcctgta gctattgctc
     6961 ccgatgcatt ggggacaatt ttcagtggct cggcagttgt cgattttgat aatacagccg
     7021 gttttggtgc aggtgccatt atcgccatct atacacaaaa cagtgaccga caagtacaaa
     7081 gcattgctta cagtactgac aacggacgta ctttcaccaa atatgagaat aatccggtac
     7141 tgacgtccga agcgcgtgac ttccgcgacc cgaaagtatt ttggtatgaa aacaccaaac
     7201 gctggatcat ggtattagca gtagggcagg agatgcagat attctcttct cctaatctga
     7261 aagattggac ctttgaaagt cgtttcggtg aagggcaagg tgcacatggt ggtgtttggg
     7321 aatgtcccga tttgtttgaa cttccagtag aaggtaccaa tgataagaaa tgggtgctgc
     7381 tttgtaattt gaatcccgga ggtcctttcg gaggaagtgc tactcaatat tttgtaggct
     7441 ccttcaacgg aaaagagttt gtaaacgaat tcccttccaa aaccaaatgg atggactggg
     7501 gaaaagacca ttacgccacc gtaacttgga gcgatgctcc cgacaaccgc cgtatcgcta
     7561 tcgcatggat gagcaactgg caatacgcaa acgatgttcc tacctcccaa taccgtagcc
     7621 cgaattccgt tccccgtgac ttgagcctgt ttaccgtaga tggtgagact tacctccaat
     7681 cagctccctc tcccgaactg ctggctttgc gtgatgcttc caagaaacgc tcgttcaaag
     7741 taaacggaac acgcacaatc aaagaaatga ttcccggcaa tgacggtgca tacgaaatag
     7801 aattgacaat cgaaaatcaa cgtgcagacg taatcggctt ccgtctctac aatgacaaag
     7861 gcgaagaagt ggatatgcag tatgacatga aagaaaagaa attctcaatg gatcgtcgca
     7921 agagcggaga ggtcagcttc aatgagaatt tcccgatgtt gacctggaca gcaattgagc
     7981 aaggtggaaa cgaggaacaa ggcggcaaag aggcattgaa actccgtctg tttgtggata
     8041 aatccagtgt ggaagccttt ggaaatggtg gacgttttgt gatgaccaat caggtattcc
     8101 cttcggaacc ttacaatcat atcaactttt acagtaaagg aggagcttat aaagtagatt
     8161 cgtttgtggt tcataagttg aagccataaa caataggcca catgcaactg taaatgttta
     8221 cgcgtcctcg gtaccaacaa atactttcag gacgatgtaa agtcctgaga aatcaaactg
     8281 aatggataga tcataatgct aatcttacac cgtcccacca catttccaat tcagaagctc
     8341 tattgtctat ccagtcatag aactcgtcac cccaagtata atcagccact ccgataccga
     8401 tagctaagac ccaaccgaca tagggaataa aagtaacgcc ataagctaaa cctgctacgg
     8461 caattcttgt acctgccacg gcaaatcgac cattttgcac atcacgcaca acttgatagc
     8521 cagtaagcac cattcctgcc catcccaaag ctttacctac tccacgcact ctactcatac
     8581 ctttagcggg gacttgatag ttccctaaag gcaatcttat aatcggcgga agcgttttgg
     8641 gcgagaaacg catctgctta ggtaagtacg cacctcgatt atccataatc ttctcagccc
     8701 aatttccggc tgtttcagca attccgaatc ctaaccctgc cagtccttcc agattgggtc
     8761 cttccttatt tacagcaggc aggctaccag aatcgcgtac cgctgacacc aagtccgtca
     8821 aactctcata ctttgcctca taagtctttc catttgagtt atccgtggca ctaatcacaa
     8881 tttcgtaccg actgccagtg tcagtaatta caatacttat ctgactatta tccgtgggca
     8941 aatattgctt ttcttggcct aaatttatct cagtgatatt attacctttg tttttaacta
     9001 ttaagtcacg gaccttttcg gaaacttttg ttggatcttg catcagactt ttttcatagg
     9061 catattggga ggtatcgtat ttcctttctg agactatcgt atgaaaactc tcgtccaccg
     9121 aaacattccg agatattgga ctccatccgt tttgatttac cggtagaatt accttgctac
     9181 tcatccaagt atatcccaag ccaactacaa atccttcata gtcatccccc cgttccaaga
     9241 tgacattaaa ttcactttgc gttttatatt catttctata agcgccggca aatgagaact
     9301 gtatagctac aatagccatc acaagcaaag ataaaatttt cttcatgtgt cttttctttt
     9361 atatgtcttt atttcgtctc tatcactgat agggagactg caaagataag aacttttagc
     9421 tttatcttcc aaactttatc cctatcagtg atagagattt ttttgttctt atggtcaagc
     9481 agtactgcgc ttttacacta agtcaattta acaaggcttt cttctcttgg aaagcgggtg
     9541 caaaaaaaga aaaggcgttt tgtttttctt ctttaccttc tttccctttc gctaagagag
     9601 tctgagaaac gatagaaaaa gaaaagcgaa aaaacttccg aaaacatttg gtagttaaaa
     9661 taaaacctct tacctttgca cccgcgcatt ttaaaataaa ataaattatt tatgatatta
     9721 aacgaatatg tctagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa
     9781 tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca
     9841 gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga
     9901 gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt
     9961 tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa
    10021 agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc
    10081 ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg
    10141 gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag
    10201 ggaaacacct actactgata gtatgccgcc attattacga caagctatcg aattatttga
    10261 tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga
    10321 aaaacaactt aaatgtgaaa gtgggtctta agatacaagc ggtcggttcg gaaatagaac
    10381 aggcacaagt aaggctgatt gttgcagcca atgcacaaat gctgttccat cactggaaga
    10441 taggcaatta gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggggatc
    10501 cactagttct agagcggccg ccaccgcggt ggaggggaat tcccatgtca gccgttaagt
    10561 gttcctgtgt cactcaaaat tgctttgaga ggctctaagg gcttctcagt gcgttacatc
    10621 cctggcttgt tgtccacaac cgttaaacct taaaagcttt aaaagcctta tatattcttt
    10681 tttttcttat aaaacttaaa accttagagg ctatttaagt tgctgattta tattaatttt
    10741 attgttcaaa catgagagct tagtacgtga aacatgagag cttagtacgt tagccatgag
    10801 agcttagtac gttagccatg agggtttagt tcgttaaaca tgagagctta gtacgttaaa
    10861 catgagagct tagtacgtga aacatgagag cttagtacgt actatcaaca ggttgaactg
    10921 ctgatcttca gatcctctac gccggacgca tcgtggccgg atcaattccg ttttccgctg
    10981 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac
    11041 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc
    11101 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc
    11161 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccggctaccg
    11221 ccggcgtaac agatgagggc aagcggatgg ctgatgaaac caagccaacc aggaagggca
    11281 gcccacctat cacggaattg atccccctcg aattg
//
