LOCUS       sequence-251807-       17868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses intact IgG2b gene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17868)
  AUTHORS   Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C
  TITLE     Transcription elongation factor ELL2 directs immunoglobulin 
            secretion in plasma cells by stimulating altered RNA processing.
  JOURNAL   Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009
            Sep 13.
  PUBMED    19749764
REFERENCE   2  (bases 1 to 17868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1786"; 
            journalName: "Nat Immunol"; date: "2009-10"; volume: "10"; issue: 
            "10"; pages: "1102-9"
COMMENT     Sequence Label: MPC11 IgG2b gene
FEATURES             Location/Qualifiers
     source          1..17868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     173..191
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    13534..13668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13558..13577)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     13612..13631
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(14093..14113)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          14243..14308
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(15443..15772)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      15457..15592
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     15757..15777
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(15815..15837)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    15923..16063
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     15978..15997
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(16078..16095)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16249..16837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16329..16348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17008..17799,17800..17868))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17631..17650
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
      181 tatcacgagg ccctttcgtc ttcaagaatt ctgctgcctg acccaggtac cttttcattc
      241 cttcctctcc agttcttctc tagatggact aggtccttaa ctaagtaatg cactgctcat
      301 gaatatgcaa atcacgcaag tctttggcag taaatactgg gatgtccaca ccctgaaaac
      361 aacctatgat cagtgtcctc tccacagtcc ctgaacacac tgactctcac catggaatgg
      421 agcggggtct ttatctttct cctgtcagta actgcaggta aggggctccc catttccaaa
      481 tctgaagagg atacagggcc tgaggtgaca atgccaacca ctctgtcttt ctctccacag
      541 gtgtccactc ccaggtccag ctgcagcagt ctggagctga gctggtaagg cctgggactt
      601 cagtgaagat gtcctgcaag gctgctggat acaccttcac taactactgg ataggttggg
      661 taaaggagag gcctggacat ggccttgagt ggattggaga tatttaccct ggaggtggct
      721 ttactaacta caatgagaat ttcaagggca aggccacact gactgcagac acatcctcca
      781 gcacagccta catgcagctc agcagcctga catctgagga ctctgccatc tatcactgtg
      841 caagagggat ttactacaat agtagcccct actttgactc ctggggccaa ggcaccactc
      901 tcacagtctc ctcaggtgag tccttacaac ctctctcttc tattcagctt aaatagattt
      961 tactgcattt gttggggggg aaatatgtgt atctgcattt caggtcatga aggactaggg
     1021 acattttggg agtcagatag ggtcattggg agccctggct gatgcagaca gacatcctca
     1081 gctcccagac tgcatggcca gagatttata gggatcctgg ccagcattgc cgctaggtcc
     1141 ctctcttcta tgctttcttt gtccctcact ggcctccatc tgagataatc ctggagccct
     1201 agccaaggat catttattgt caggggtcta atcattgttg tcacaatgtg cctggtttgc
     1261 ttactggggc caagggactc tggtcactgt ctctgcaggt gagtcctaac ttctcccatt
     1321 ctaaatgcat gttgggggga ttctgagcct tcaggaccaa gattctctgc aaacgggaat
     1381 caagattcaa cccctttgtc ccaaagttga gacatgggtc tgggtcaggg actctctgcc
     1441 tgctggtctg tggtgacatt agaactgaag tatgatgaag gatctgccag aattgaagct
     1501 tgaagtctga ggcagaatct tgtccagggt ctatcggact cttgtgagaa ttaggggctg
     1561 acagttgatg gtgacaattt cagggtcagt gactgtctgg tttctctgag gtgaggctgg
     1621 aatataggtc accttgaaga cttaagaggg gtccaggggg cttctgcaca ggcagggaac
     1681 agaatgtgga acaatgactt gaatggttga ttcttgtgtg acaccaggaa ttggcataat
     1741 gtctgagttg cccaggggtg attctagtca gactctgggg tttttgtcgg atatagagga
     1801 aaaatccact attgtgatta ctttgctatg gactactggg gtcaaggaac ctcagtcacc
     1861 gtctcctcag gtaagaatgg cctctccagg tctttatttt taacctttgt tatggagttt
     1921 tctgagcatt gcagactaat cttggatatt tgtccctgag ggagccggct gagagaagtt
     1981 gggaaataaa ctgtctaggg atctcagagc ctttaggaca gattatctcc acatctttga
     2041 aaaactaaga atctgtgtga tggtgttggt ggagtccctg gatgatggga tagggacttt
     2101 ggaggctcat ttgagggaga tgctaaaaca atcctatggc tggagggata gttggggctg
     2161 tagttggaga ttttcagttt ttagaataaa agtattagct gcggaatata cttcaggacc
     2221 acctctgtga cagcatttat acagtatccg atgcataggg acaaagagtg gagtggggca
     2281 ctttctttag atttgtgagg aatgttccac actagattgt ttaaaacttc atttgttgga
     2341 aggagagctg tcttagtgat tgagtcaagg gagaaaggca tctagcctcg gtctcaaaag
     2401 ggtagttgct gtctagagag gtctggtgga gcctgcaaaa gtccagcttt caaaggaaca
     2461 cagaagtatg tgtatggaat attagaagat gttgctttta ctcttaagtt ggttcctagg
     2521 aaaaatagtt aaatactgtg actttaaaat gtgagagggt tttcaagtac tcattttttt
     2581 aaatgtccaa aatttttgtc aatcaatttg aggtcttgtt tgtgtagaac tgacattact
     2641 taaagtttaa ccgaggaatg ggagtgaggc tctctcatac cctattcaga actgactttt
     2701 aacaataata aattaagttt aaaatatttt taaatgaatt gagcaatgtt gagttggagt
     2761 caagatggcc gatcagaacc agaacacctg cagcagctgg caggaagcag gtcatgtggc
     2821 aaggctattt ggggaaggga aaataaaacc actaggtaaa cttgtagctg tggtttgaag
     2881 aagtggtttt gaaacactct gtccagcccc accaaaccga aagtccaggc tgagcaaaac
     2941 accacctggg taatttgcat ttctaaaata agttgaggat tcagccgaaa ctggagaggt
     3001 cctcttttaa cttattgagt tcaacctttt aattttagct tgagtagttc tagtttcccc
     3061 aaacttaagt ttatcgactt ctaaaatgta tttagaattc attttcaaaa ttaggttatg
     3121 taagaaattg aaggacttta gtgtctttaa tttctaatat atttagaaaa cttcttaaaa
     3181 ttactctatt attcttccct ctgattattg gtctccattc aattcttttc caatacccga
     3241 agcatttaca gtgactttgt tcatgatctt ttttagttgt ttgttttgcc ttactattaa
     3301 gactttgaca ttctggtcaa aacggcttca caaatctttt tcaagaccac tttctgagta
     3361 ttcattttag gagaaatact ttttttttaa atgaatgcaa ttatctagac ttatttcggt
     3421 tgaacatgct ggttggtggt tgagaggaca ctcagtcagt cagtggcgtg aagggcttct
     3481 aagccagtcc acatgctctg tgtgaactcc ctctggccct gcttattgtt gaatgggcca
     3541 aaggtctgag accaggctgc tgctgggtag gcctggactt tgggtctccc acccagacct
     3601 gggaatgtat ggttgtggct tctgccaccc atccacctgg ctgctcatgg accagccagc
     3661 ctcggtggct ttgaaggaac aattccacac aaagactctg gacctctccg aaaccaggca
     3721 ccgcaaatgg taagccagag gcagccacag ctgtggctgc tgctcttaaa gcttgtaaac
     3781 tgtttctgct taagagggac tgagtcttca gtcattgctt tagggggaga aagagacatt
     3841 tgtgtgtctt ttgagtaccg ttgtctgggt cactcacatt taactttcct tgaaaaacta
     3901 gtaaaagaaa aatgttgcct gttaaccaat aatcatagag ctcatggtat tttgaggaaa
     3961 tcttagaaaa cgtgtataca attgtctgga attatttcag ttaagtgtat tagttgaggt
     4021 actgatgctg tctctacttc agttatacat gtgggtttga attttgaatc tattctggct
     4081 cttcttaagc agaaaattta gataaaatgg atacctcagt ggtttttaat ggtgggttta
     4141 atatagaagg aatttaaatt ggaagctaat ttagaatcag taaggaggga cccaggctaa
     4201 gaaggcaatc ctgggattct ggaagaaaag atgtttttag tttttataga aaacactact
     4261 acattcttga tctacaactc aatgtggttt aatgaatttg aagttgccag taaatgtact
     4321 tcctggttgt taaagaatgg tatcaaagga cagtgcttag atccaaggtg agtgtgagag
     4381 gacaggggct ggggtatgga tacgcagaag gaaggccaca gctgtacaga attgagaaag
     4441 aatagagacc tgcagttgag gccagcaggt cggctggact aactctccag ccacagtaat
     4501 gacccagaca gagaaggcca gactcataaa gcttgctgag caaaattaag ggaacaaggt
     4561 tgagagccct agtaagcgag gctctaaaaa gcatggctga gctgagatgg gtgggcttct
     4621 ctgagcgctt ctaaaatgcg ctaaactgag gtgattactc tgaggtaagc aaagctgggc
     4681 ttgagccaaa atgaagtaga ctgtaatgaa ctggaatgag ctgggccgct aagctaaact
     4741 aggctggctt aagcgagatg agccaaactg gaatgaactt cattaatcta ggttgaatag
     4801 agatgtgagg taccagtcct agcagctatg gggcaactgg gtataattgg aatatggggg
     4861 accagatcta gcagctatgg aggagcaggg ataggtggga gtattaggga ccagtcctat
     4921 cagctgtggg ggagctgaag atggtaggaa tgtggaggac cagacctaag agttaggaga
     4981 gagctggggc aggtaggagt atgagggacc agtactaaca gctatggagg agttggggat
     5041 tttggaaatg ttgagagact agtcctagca gctatggggc aactgagtat agtttgaata
     5101 tgggggacta tatctagcag ctatggggaa gcaaggatag gtgggagtat tagggaccaa
     5161 tcccagcagc tgtgggggag ttggggatgg tagcaatgtg gggaaccagt cctagaagct
     5221 atgggggatc tggaataggt agtagagtca gggacaagac atagcagcta tgatggagct
     5281 ggagaaggtg ggaatatgag ggagaagtcc tagcagctat ggaggaacta tggatcatag
     5341 aaatttgggg gaccagttct agaagttgtg ggagagctga ggcaggtgga agtgtgaggg
     5401 accagacctg acagctatgg agggcttggg atgttgaaaa tgtgagggac cagttctagc
     5461 agctatgggg aatcttggtc aggtgagggt gattttaagg gaccagacct agtaggtata
     5521 ggtctgctgg gcaggttaga gtgtcaggaa ccaactatga aagacctgag ttaggtagga
     5581 gtgtaaggga ccagacatag cagctaaggg gaaatgtgat aaatgggagt gtaagggacc
     5641 agattcagta aactttggag tataggtgag catccagaaa atctgtggtg caactgtaac
     5701 aggtggggtg tgaggaacca gacccagtaa gaatgggata cctggggaga gcggcatgtg
     5761 agggaccacc agtggttaag gtagcaaacc tgctcatgga aagcttccag agatgagagc
     5821 agagaacagg acagtgtagg atgtgcagga tctagaagct catctcagag gatgaagaac
     5881 agtcaaaagc tctgctcaga agttcttcag ggcgatgtga acatgctggg tactcaggaa
     5941 gtgacaagga tcctccctta cacacctcat aaaacaagag agagtggggg ctcaggcaga
     6001 cacaacagct gtgccaaaag tgaataagga tgaaaagagg caacaggaag agaagaaaaa
     6061 gggatgggaa agcactccgg gctctactga attcaggcag aagaatagct tggctgcaga
     6121 gagtcctggg ggccaggaga gttgtccgat tgagcaggaa cataggcagg aaaaggccct
     6181 ggcactgaca ttctcaagca agccgtataa gttaaagttc tagtgtcaga ggcccaagct
     6241 cggatcctcg aacattatgg aaacataagg caggaggtga aaccttgtgt gagaacatga
     6301 agcagcctga gttggcccac agaagagagg tggggtaact ctcagagtgt gagcagggga
     6361 ttgtggaaga tagtgtacag gagagatgga aagaccagaa aggacctggg agtaagaatg
     6421 aatcaagcat ttatgtgtgg ttttgttcaa tgaaagaaaa ggcagggaaa attcatgaca
     6481 aatctctttt gctatccaga actcattgtt caatacgagt tttgatacat acaagaaaaa
     6541 tatgttataa atgaaaagaa tgaaccagaa tcaaaagcaa tggaagacta catgtcttct
     6601 agaagaatgc agcatacact cctatttcaa ggggtactgg caagagaaaa ggactttcct
     6661 tgggactggc cacattagca ctattaggga ggacacagaa cagtgtaaag aatgaggaag
     6721 gtgaaggaag gtggctgagt ttaacaggtt ctcatcaaga ggtctgagct gtgtagaaca
     6781 agtaacagat agtagccagc tacatcaaga gagaaaggct gcccacacag ggaaagcata
     6841 gggtaggagg ttgttggtta tcctcagaaa gcagggacca aggtccaagg agagctaaac
     6901 ccatggatag agacctagaa ccaagattta ggagccagag cttgaataag catagtagac
     6961 taaaaagggg cttctgaaag gcatcatcac taaaggatca gggttcatgg gactagagag
     7021 atggctgagt ggtgaaaagg aatagctact ctttcagagg acccaggttc agctaacaac
     7081 aaccagatgg catcttacaa tggactgtaa gaaacaccag tttctgggta ttcaccgctc
     7141 ctttctacca gcacagccat cgggcacaca cataatacac aaattcacac atcgaaaata
     7201 aaaaacatgg aaaaattaag gtcacagtgc aagctctgga ggtggaaatt tctgacactg
     7261 cttgcctcag atcaattgta gaagacacgg ttctcaagag caaagctaag aacagaatct
     7321 ctccaaatat ccgaggccac tgataagaaa aagctcacac attctcctct cttgcagcca
     7381 aaacaacacc cccatcagtc tatccactgg cccctgggtg tggagataca actggttcct
     7441 ccgtgactct gggatgcctg gtcaagggct acttccctga gtcagtgact gtgacttgga
     7501 actctggatc cctgtccagc agtgtgcaca ccttcccagc tctcctgcag tctggactct
     7561 acactatgag cagctcagtg actgtcccct ccagcacctg gccaagtcag accgtcacct
     7621 gcagcgttgc tcacccagcc agcagcacca cggtggacaa aaaacttggt gagaggacat
     7681 tcaggggagg agggattcac cagagttgag gcaaaggtat tagcctatct aaaccagcca
     7741 ggctgggatc catcaccaag gaggtgacct tagcccaggg aagagggaga tactgtctct
     7801 gcctccctcc tgggaacatc tagctatgac cacctacact caaggacatg atcctctggg
     7861 ataggtgtgc ttgtcatttc caggatcatc ctggactaag cccataccag ggacaaactt
     7921 tcctctctct ggtttggtgc ttctctcctt caaaaaccag taacatccag ccttctctct
     7981 gcagagccca gcgggcccat ttcaacaatc aacccctgtc ctccatgcaa ggagtgtcac
     8041 aaatgcccag gtaagtcact accagagctc cactcccagg agaatggtaa gtgctgtaaa
     8101 aatccctgta atggaggata agccatgtac aaatccattt ccatctctcc tcatcagctc
     8161 ctaacctcga gggtggacca tccgtcttca tcttccctcc aaatatcaag gatgtactca
     8221 tgatctccct gacacccaag gtcacgtgtg tggtggtgga tgtgagcgag gatgacccag
     8281 acgtccagat cagctggttt gtgaacaacg tggaagtaca cacagctcag acacaaaccc
     8341 atagagagga ttacaacagt actatccggg tggtcagcac cctccccatc cagcaccagg
     8401 actggatgag tggcaaggag ttcaaatgca aggtcaacaa caaagacctc ccatcaccca
     8461 tcgagagaac catctcaaaa attaaaggtg ggacctgcag gacaactgca tgggggctgg
     8521 gatgggcata agaataaatg tctatgtgga cagccttcca cttcagccat gacctctatg
     8581 tatgtttcta accccacagg gctagtcaga gctccacaag tatacatctt gccgccacca
     8641 gcagagcagt tgtccaggaa agatgtcagt ctcacttgcc tggtcgtggg cttcaaccct
     8701 ggagacatca gtgtggagtg gaccagcaat gggcatacag aggagaacta caaggacacc
     8761 gcaccagtcc tggactctga cggttcttac ttcatatata gcaagctcaa tatgaaaaca
     8821 agcaagtggg agaaaacaga ttccttctca tgcaacgtga gacacgaggg tctgaaaaat
     8881 tactacctga agaagaccat ctcccggtct ccgggtaaat gagctcagca cccacaaagc
     8941 tctcaggtcc taagagacac tggcacccat atccatgcat cccttgtata aataaagcat
     9001 ccagcaaagc ctggtaccat gtaaaactgt cctggttctt tccaaggtat agagcatagc
     9061 tcacgggctg ataggtctgg ccagggttgg agaacagcct tgtctatagg aagagaatga
     9121 ggtttctgca ctgcatgact cagaggtcat gagtcattct gccttggact gttggggctt
     9181 ggctttaggc agtgcctttt ccttgccttc ccatgaacca gcagctgcca gacatagaga
     9241 taatcctagg aagcctcaaa tggataagca cacaagccca cctcccttag gctgttcctg
     9301 tccccctgcc ccatttctct acttaggggt ttttctgagt ctattgtgga gtgacacatg
     9361 gccaggggca ttccatagac ctttgtcatc tatactctca actcaggcag cttttcacag
     9421 acgaggtctg cacacccata caaatggctc actcctgcct gtgccacctg gggctgaggc
     9481 acatggctct tgatgcccca agggagggac tattagatag ccacactcat gctaaatcct
     9541 gaccctgctg aacaccatcc agtccatata gcacatgtat cctcatgctc gtgagcacac
     9601 acacatttaa cacactggga caactcctct gtgccctgca cagcacctac atccagcaat
     9661 gtatcaccat acactcagcc aaaaattcaa tgcccacgtt tctttcatca caatcagaca
     9721 cacctttcct cttctgtggc cactgcatta tatgctcaac acaagacctc tgaagccaga
     9781 tccatctctg gtacctcggg gtcatgcttc aaccccacat gaataattca aaccatagcc
     9841 ataattgtct gaatcacttc acactgggat gttcccaagt tcaggcaaga cgagccacag
     9901 gctctgctga tgactgaagg acagcaaagg gtcagtccag ctgtatagcc actgttgacc
     9961 tgggtcacag gccctgctga ccctccacct tctcctgtac tgaaggaatg aaagatgaga
    10021 caagcataga gggcacttga ataatccagg tcactctgag gtccacccaa ggcattattg
    10081 gactcaggtg ggaagctgag actgatgtcc cagacggaaa ggaaggaaag caggccccgg
    10141 ggagggtctg ctgtcccagt caggctggag atctctcctc tgacttcatg cagacatgtc
    10201 tgcctcacag ggaatctctc ccagcatcaa ccatgttggg acaaacactg actgtcctct
    10261 ctgttcaggg ctagacctgg atgatatctg tgctgaggcc aaggatgggg agctggacgg
    10321 cctctggacg accatcacca tcttcatcag cctcttcctg ctcagcgtgt gctacagcgc
    10381 ctctgtcaca ctcttcaagg ttggcactgt ctcccaccct ctgctgtgat agctacactg
    10441 accacaaaat gtcctctcac tcctcccctg atgtagtagg atgttacttt gctgccccca
    10501 ctctgtacca cacaccgttt cctccattcc ctgagccatc ccacattgct ctatgtgact
    10561 ccacattttg tcccatacag tctgcccttc tgtctctact gctgtcctgc atgatcctca
    10621 tactgtctta tgcaaccaaa cctccttgca ttccacacta gccttcatga ggttcatgct
    10681 gtctaacaca caatcccctt gatgttcgcc tcaccataac tcaaggtact ctgtgagcta
    10741 atctcatacc atctccacct caactcccac aatatctcca ctttgaccac ttcccatacc
    10801 cagtctccta cctgtatgaa aggaattgaa gaagagacag gcctctgtct tccccacagg
    10861 attggagggt ctgagcatgg gtgtggtctc tgactctcac ttccccacag gtaaagtgga
    10921 tcttctcctc agtggtggag ctgaagcaga agatctcccc tgactacaga aacatgattg
    10981 ggcagggagc ctaggccact tcctctggga tcagaagagc ttcctaagtc ctgcagaagc
    11041 ccatccatcc tactgtgcag cctaacaggg aggccacact ctagccctat gactctctga
    11101 tccagaactc tccatggtct ctcctttgga ggaccatgtg cagagcaggc tttaccccag
    11161 acctaaacac ttccacagca gttgccaaat aactaactga tcagcgccag aataaccatc
    11221 tccatccaaa ccagcactag gggatctgag atctcagaat gtttgcctaa gatgaactgg
    11281 aaatccaggt ttcctgagtt cagctgcatt gacatcagcc ctggatttgg cctggaccac
    11341 acattttcat ctgaaatgga tcttcccaga aagggcaatg ttttcagata cttgaatgga
    11401 gacatctgct cagaaggcca gggtcacaat agcccaaagg tcactctggt atcctttgta
    11461 ccagctatac ttggacagct tccttcctgg tacttctgtg aaccctgggc tgaggaaagg
    11521 acctgtgatc ctcaaagtgg tgggatgagc cctctgaaga agatgcagtc ttctcttcct
    11581 cctacccact caagatgctg ggttctcaga tacagaatcc agtccacaga cactgttgtc
    11641 cacagaactt caagacaagg ccactgccac ctccagaggc agaaggactg ctttgtgtag
    11701 cctcacaaag ctctagcagg gacaagtgta tacaccactg agtactggct aggtaatcag
    11761 gcaggaaaag ttccaggtga gggctctgat gagaagattc ctgttgggct caataaaatg
    11821 ggggttgggg agaggagcaa tgtgggagag atgtgagcac atagttaaaa gaaaaccatg
    11881 gtgccacagg tctgctctga aggcctggtg tccagactgg accactcaca gcagaaaaca
    11941 tcagttgcaa tggacctcta ggtggacaga tctagaatac cagggtgatt ttagctaatg
    12001 aaaagtagcc ctgtatcctt tcctcttcta cctgcctagg acaataagaa gccagagtct
    12061 ccagacagaa agaaagagca cgcacttgct tttgcgttaa tgggtctgat aggaaatgtt
    12121 tagagaactt ggacacttaa gagctgtgga tttttctaga ggtcatgaat gtcttaaagt
    12181 gttctctctc cctctctctc tctctctcag tgtctctttg tgtgtatgtg tgtatgtgtg
    12241 tttgtgtgca tgtgtgcgtg cacatgcggg cgcgcacagg tgcacactca tgcatgctgt
    12301 tcttttggaa tttgaaacaa taaaagattc ttttaatatt gtgtatctca tggtgatcct
    12361 tatgtgtgga tcagccctaa catccagaaa cagggggaaa taaacagacc agagccatat
    12421 acacccctct ccctccctcc tgatcctgat ttcccagagc tccctcagtg ggcccctcac
    12481 accagggtcc catccctggc agagccacaa aaggctaagg cacctcctcc acacagaaag
    12541 gttttttcct agctggaggc tgtcctgatg ccatgggcag gctgtggagc agagttgatg
    12601 accacggtgg actccaggca agccaggaaa agcctcccat gccagctgag aagcaagtgg
    12661 ctctcactga ccttgggctg ctatagatta cacaaaagac atggatccag ctacctccat
    12721 agcagatact tattgtctca agccatattt agagacattc cagatcaggc actgacaggg
    12781 tgaattcctt tgcctaattt aaatgaggac ttaacctgtg gaaatatttt gatgtgggaa
    12841 gctgttactg ttaaaactga ggttattggg gtaactgcta tgttaaactt gcattcaggg
    12901 acacaaaaaa ctcatgaaaa tggtgctgga aaacccattc aagggtcaaa ttttcatttt
    12961 tttgctgttg gtggggaacc tttggagctg cagggtgtgt tagcaaacta caggaccaaa
    13021 tatcctgctc aaactgtaac cccaaaaaat gctacagttg acagtcagca gatgaacact
    13081 gaccacaagg ctgttttgga taaggataat gcttatccag tggagtgctg ggttcctgat
    13141 ccaagtaaaa atgaaaacac tagatatttt ggaacctaca caggtgggga aaatgtgcct
    13201 cctgttttgc acattactaa cacagcaacc acagtgcttc ttgatgagca gggtgttggg
    13261 cccttgtgca aagctgacag cttgtatgtt tctgctgttg acatttgtgg gctgtttacc
    13321 aacacttctg gaacacagca gtggaaggga cttcccagat attttaaaat tacccttaga
    13381 aagcggtctg tgaaaaaccc ctacccaatt tcctttttgt taagtgacct aattaacagg
    13441 aggacacaga gggtggatgg gcagcctatg attggaatgt cctctcaagt agaggaggtt
    13501 agggtttatg aggacacaga ggagcttcct ggggatccag acatgataag atacattgat
    13561 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
    13621 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
    13681 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
    13741 aacctctaca aatgtggtat ggctgattat gatctctagt caaggcacta tacatcaaat
    13801 attccttatt aaccccttta caaattaaaa agctaaaggt acacaatttt tgagcatagt
    13861 tattaatagc agacactcta tgcctgtgtg gagtaagaaa aaacagtatg ttatgattat
    13921 aactgttatg cctacttata aaggttacag aatatttttc cataattttc ttgtatagca
    13981 gtgcagcttt ttcctttgtg gtgtaaatag caaagcaagc aagagttcta ttactaaaca
    14041 cagcatgact caaaaaactt agcaattctg aaggaaagtc cttggggtct tctacctttc
    14101 tcttcttttt tggaggagta gaatgttgag agtcagcagt agcctcatca tcactagatg
    14161 gcatttcttc tgagcaaaac aggttttcct cattaaaggc attccaccac tgctcccatt
    14221 catcagttcc ataggttgga atctaaaata cacaaacaat tagaatcagt agtttaacac
    14281 attatacact taaaaatttt atatttacct tagagcttta aatctctgta ggtagtttgt
    14341 ccaattatgt cacaccacag aagtaaggtt ccttcacaaa gatccggggc ccactcataa
    14401 atccagttgc cgccacggta gccaatcacc gtatcgtata aatcatcgtc ggtacgttcg
    14461 gcatcgctca tcacaatacg tgcctggacg tcgaggattt cgcgtgggtc aatgccgcgc
    14521 cagatccaca tcagacggtt aatcatgcga taccagtgag ggatggtttt accatcaagg
    14581 gccgactgca caggcggttg tgcgccgtga ttaaagcggc ggactagcgt cgaggtttca
    14641 ggatgtttaa agcggggttt gaacagggtt tcgctcaggt ttgcctgtgt catggatgca
    14701 gcctccagaa tacttactgg aaactattgt aacccgcctg aagttaaaaa gaacaacgcc
    14761 cggcagtgcc aggcgttgaa aagattagcg accggagatt ggcgggacga atacgacgcc
    14821 catatcccac ggctgttcaa tccaggtatc ttgcgggata tcaacaacat agtcatcaac
    14881 cagcggacga ccagccggtt ttgcgaagat ggtgacaaag tgcgcttttg gatacatttc
    14941 acgaatcgca accgcagtac caccggtatc caccaggtca tcaataacga tgaagccttc
    15001 gccatcgcct tctgcgcgtt tcagcacttt aagctcgcgc tggttgtcgt gatcgtagct
    15061 ggaaatacaa acggtatcga catgacgaat acccagttca cgcgccagta acgcacccgg
    15121 taccagaccg ccacggctta cggcaataat gcctttccat tgttcagaag gcatcagtcg
    15181 gcttgcgagt ttacgtgcat ggatctgcaa catgtcccag gtgacgatgt atttttcgct
    15241 catgtgaagt gtcccagcct gtttatctac ggcttaaaaa gtgttcgagg ggaaaatagg
    15301 ttgcgcgaga ttatagagat ctggcgcact aaaaaccagt atttcacatg agtccgcgtc
    15361 tttttacgca ctgcctctcc ctgacgcggg ataaagtggt attctcaaac atatctcgca
    15421 agcctgtctt gtgtccaagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact
    15481 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
    15541 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
    15601 ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
    15661 ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca
    15721 tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac acattccaca
    15781 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
    15841 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
    15901 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
    15961 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    16021 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
    16081 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    16141 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    16201 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    16261 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    16321 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    16381 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    16441 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    16501 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    16561 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    16621 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    16681 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    16741 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    16801 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    16861 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    16921 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    16981 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    17041 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    17101 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    17161 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    17221 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    17281 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc
    17341 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    17401 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    17461 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    17521 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    17581 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
    17641 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    17701 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    17761 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    17821 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcat
//
