LOCUS       sequence-25181-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.63
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..112
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    242..365
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(436..453)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(436..452)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(445..467)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      516..602
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgctnnnngg agctccnaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
       61 caaattgata agcaatgctt ttttataatg ccaactttgt ataataaagt tgcagaattg
      121 aatagcaaat aaatcacaaa agttccaatt tctcgattta tttcagttcg aagtcataat
      181 aaacacaatg gatacataat taatattttc cgcttaaaac aaaaactccg attgcttcat
      241 cccactttgt acaagaaagt tgaacgagaa acgtaaaatg atataaatat caatatatta
      301 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatgcagtca
      361 ctatgaacca actacttaga tggtattagt gacctgtaga attcgagctc tagagctgca
      421 gggcccgagc ttaagactgg ccgtcgtttt acaacgtcgt gactgggaaa acatccatgc
      481 tagcgttaac gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg
      541 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
      601 atccgccggg agcggatttg aacgttgtga agcacnncnn nnnnngnnnn gnnngggcag
      661 ganncccngc cataaactgc caggcatcan nnnnaagcag aaggncatcc ctgacggatg
      721 gcctnttttg cgtttctaca aactcttcct ggctagcggt acgcgtatta attngcgttg
      781 cgctcactgc ccgcttncca gtcgggaaac ctgtcgtgnc agctgcattn ntgaatcnnn
      841 cnacnc
//
