LOCUS       sequence-251828-        9681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human C1ORF43 with C-terminal GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Jeng EE, Bhadkamkar V, Ibe NU, Gause H, Jiang L, Chan J, Jian R, 
            Jimenez-Morales D, Stevenson E, Krogan NJ, Swaney DL, Snyder MP, 
            Mukherjee S, Bassik MC
  TITLE     Systematic Identification of Host Cell Regulators of Legionella 
            pneumophila Pathogenesis Using a Genome-wide CRISPR Screen.
  JOURNAL   Cell Host Microbe. 2019 Oct 9;26(4):551-563.e6. doi: 
            10.1016/j.chom.2019.08.017. Epub 2019 Sep 17.
  PUBMED    31540829
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2019.08.017"; journalName: "Cell Host Microbe"; 
            date: "2019-10-9- 9"; volume: "26"; issue: "4"; pages: "551-563.e6"
COMMENT     Sequence Label: C1ORF43-GFP
FEATURES             Location/Qualifiers
     source          1..9681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(56..75)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        247..626
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        628..826
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     781..801
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             844..1024
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1071..1196
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1693..1926
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2111..2155
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2453..2570
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2823..2856)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2860..2880
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        3125..3336
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3337..4275
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4232..4252
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4296..4892
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4296..4315)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4792..4812
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4902..4955
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4962..4971
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5739..5741,5742..5744,5745..6458)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5784..5805)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6045..6064)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6392..6413
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6481..6514)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6570..7158
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6623..6643)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7041..7052)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7161..7177)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7162..7178)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7687..7867
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7929..8517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8009..8028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8688..9479,9480..9548))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9311..9330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9549..9653)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
     1561 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1621 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1681 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1741 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1801 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1861 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1921 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1981 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     2041 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     2101 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     2161 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     2221 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     2281 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     2341 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2401 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     2461 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2521 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2581 acagggacag cagagatcca gtttggttag taccgggccc gctctagaga tccgacgcgc
     2641 catctctagg cccgcgccgg ccccctcgca cggacttgtg ggagaagctc ggctactccc
     2701 ctgccccggt taatttgcat ataatatttc ctagtaacta tagaggctta atgtgcgata
     2761 aaagacagat aatctgttct ttttaatact agctacattt tacatgatag gcttggattt
     2821 ctataacttc gtatagcata cattatacga agttataaac agcacaaaag gaaactcacc
     2881 ctaactgtaa agtaattgtg tgttttgaga ctataagtat cccttggaga accaccttgt
     2941 tggaccagga tgggcaccac ccgtttaaga gctaagctgg aaacagcata gcaagtttaa
     3001 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttctcga
     3061 gtactaggat ccattaggcg gccgcgtgga taaccgtatt accgccatgc atgtgcccgt
     3121 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     3181 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     3241 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     3301 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     3361 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     3421 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     3481 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     3541 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     3601 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     3661 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     3721 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     3781 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     3841 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     3901 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     3961 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     4021 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgcgcttt
     4081 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     4141 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     4201 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     4261 tttcttccat ttcaggtgtc gtgagctagc ccaccatgac cgagtacaag cccacggtgc
     4321 gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg
     4381 actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc
     4441 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     4501 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     4561 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     4621 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     4681 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     4741 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     4801 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     4861 gcacctggtg catgacccgc aagcccggtg ccggatcggg agagggcaga ggaagtctgc
     4921 taacatgcgg tgacgtcgag gagaatcctg gcccaccggt cgccaccatg gcgtccggca
     4981 gtaactggct ctccggggtg aatgtcgtgc tggtgatggc ctacgggagc ctggtgtttg
     5041 tactgctatt tatttttgtg aagaggcaaa tcatgcgctt tgcaatgaaa tctcgaaggg
     5101 gacctcatgt ccctgtggga cacaatgccc ccaaggactt gaaagaggag attgatattc
     5161 gactctccag ggttcaggat atcaagtatg agccccagct ccttgcagat gatgatgcta
     5221 gactactaca actggaaacc cagggaaatc aaagttgcta caactatctg tataggatga
     5281 aagctctgga tgccattcgt acctctgaga tcccatttca ttctgaaggc cggcatcccc
     5341 gttccttaat gggcaagaat ttccgctcct acctgctgga tctgcgaaac actagtacgc
     5401 ctttcaaggg tgtacgcaaa gcactcattg ataccctttt ggatggctat gaaacagccc
     5461 gctatgggac aggggtcttt ggccagaatg agtacctacg ctatcaggag gccctgagtg
     5521 agctggccac tgcggttaaa gcacgaattg ggagctctca gcgacatcac cagtcagcag
     5581 ccaaagacct aactcagtcc cctgaggtct ccccaacaac catccaggtg acatacctcc
     5641 cctccagtca gaagagtaaa cgtgccaagc acttccttga attgaagagc tttaaggata
     5701 actataacac attggagagt actctgggtg gaagtggtat ggtgagcaag ggcgaggagc
     5761 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     5821 tcagcgtgtc tggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     5881 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     5941 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     6001 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     6061 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     6121 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     6181 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggcg aacttcaaga
     6241 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     6301 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     6361 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     6421 ccgggatcac tctcggcatg gacgagctgt acaagtaata agaattcgtc gagggaccta
     6481 ataacttcgt atagcataca ttatacgaag ttatacatgt ttaagggttc cggttccact
     6541 aggtacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     6601 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6661 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6721 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6781 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6841 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6901 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6961 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7021 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7081 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7141 tttgggccgc ctccccgcat cgataccgtc gacctcgatc gagacctaga aaaacatgga
     7201 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     7261 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     7321 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7381 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     7441 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     7501 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     7561 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     7621 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     7681 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7741 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7801 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7861 tctagcagca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7921 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7981 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8041 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8101 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8161 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8221 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8281 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8341 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8401 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8461 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8521 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8581 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8641 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8701 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8761 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8821 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8881 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8941 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9001 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9061 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9121 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9181 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9241 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9301 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9361 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9421 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9481 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9541 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9601 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9661 ccccgaaaag tgccacctga c
//
