LOCUS       sequence-251837-        8465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the PhiC31 landing pad on hThal5.10-2-hg18 with hygromycin 
            resistance cassette coexpressed with mRuby2 as a type 0 part to be 
            used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8465)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 8465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK0_052
FEATURES             Location/Qualifiers
     source          1..8465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    209..242
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(1240..2055)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1240..2055)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1963..1982
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2047..2066
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2280..2868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2769..2788
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    3938..3971
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    4589..4622
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        4644..4947
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4949..5224
                     /label=chicken beta-actin promoter
     intron          5225..6241
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6165..6187
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6249..6268
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6353..7372
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6353..7372
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7388..7441
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7448..7468
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7484..8191
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     primer_bind     complement(8352..8371)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8406..8425
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gttacagaga tgttacgaac cctgaacagg ttgactatta attgtgtctg cttgatgtgg
       61 acaccaggtg gcgctggaca tcagatttgg agaggcagtt gtctagggaa ccgggctctg
      121 tgccagcgca ggaggcaggc tggctctcct attccaggga tgctcatcca ggaaggaaag
      181 gttgcatgct ggacacacta accttacaga agttcctatt ctctagaaag tataggaact
      241 tcgagtgttt atacagttgc cttacaaaga cagtattaga ataaaaaaag cctcaggcac
      301 ttctattata ataatgaatg tctcatgaga tgggatgatg tgcaggttac aacattctga
      361 tagtaatttc taatgcatta cagtagagca tggactcttg agaatcagat gaaattttct
      421 tccatctaga ttacattttc tcactgtccc aatgtgcttg atatgaactg acaccaccat
      481 aaatactttt atctgaatgg cagcactgtc atagttagac agagaaatat atagcgttga
      541 gtgaaaaata attttagata ctttccaagt gctttttgct gttgtaacag taattcctgc
      601 aaagtacata ctaaaccatg taaaattaca atcctgacta ttgagttagt gaattatgta
      661 tactttgagg aaagcggaaa atgtgtattc agatagtatg taagatagct gcgctttctg
      721 gaggcttata gatctacata ttgcctatgt aatcactcga ggaaggaaac caagtgctct
      781 tgcccttcta atgaattttg aatgcatcag taatcactgt attatgtatc tccaattaac
      841 tagagaaaca ggctcacaat attctaaatt tgtacactaa ggaagtacat aacactgtgt
      901 tgttaattga gaaaatactc attccaacaa ggttaacaac cctaaaggag ttaaaattta
      961 tcaagtgctt gaacaatgca tagcacttat taagtgctaa acaatttgtt gacaattaaa
     1021 tattcactgt ttgaattttg taaattgcat tttcttcata aaacactgca taaatttgtc
     1081 tcatatttta aattatgact ttcctttttt tcttcaatga gtctaaataa cattggtggt
     1141 gttagatatg attgaactct attattgatt gaattcacta agcaaattat ccagcttttt
     1201 ccgagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat
     1261 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     1321 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     1381 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1441 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1501 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1561 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac
     1621 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1681 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1741 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1801 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1861 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1921 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1981 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg
     2041 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     2101 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     2161 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct
     2221 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2281 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2341 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2401 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2461 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2521 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2581 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2641 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2701 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2761 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2821 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2881 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2941 ttatcccctg attctgtgga taaccgtgcg gccgccaatt agaaatgtat gaattaaatt
     3001 ggattaatca caatatatac tatataaaaa agtatgcagt acataaatac acagatacaa
     3061 aatataggca tcatttttga agttatgtta taatatattg aggctatttt attagaaaac
     3121 gaactttatg ctaactgtat aaaggaaaaa agaataattg cagaagaaat aatttataat
     3181 ttgcttttcc ttggttttat ttaaaaatta aattaattaa attataagta tattgtttcc
     3241 atataattta taataaataa ggcatatagg ccaggcacgg tggctcatgc ctgtaatccc
     3301 agcactttgg gaggcagagg tgggtgaacg cctgaggtca ggcgttcaag accagcctgg
     3361 ccaacatggt gaaacctcgt ctgtacaaaa aatacaaaaa tcagctgggc atggtgtcag
     3421 atgcctgtaa tcccaggtac ttgggaggct gaggcaggag aatctcttga acccaggagg
     3481 cagaggttgc agtgagccga gatcatgcca ttgcactcct gcctggggga caagagcgag
     3541 acttcgtcta aaaaacaaaa caaaacaaaa caaaaacaaa aaaaaacaca aagaaacaaa
     3601 aaaccattta aagcaacaaa ttactcattt cttagctata ttctgtttaa ttaaaaaata
     3661 gggtatcctc tatgtaaaga aaaatataat gttattgagc cattttcttc agaagaacaa
     3721 gctactctac ctatgtagag tgataatgta tcctcaggat tgaagatcaa aagaggtaat
     3781 atatacaaac attctttgaa aggtgcctta taaaacaaaa aattgtttta tcataaaaat
     3841 ttaaggtagt gcagagagct aacatgaaaa atgtaaacat tggcaataag aacaaagata
     3901 tactatgttt gaaaaatggt tgaagtccct ttcccctgaa gttcctattc tctagaaagt
     3961 ataggaactt cctgaactgg ccgataattg cagacgatcg aattcgagtt ggcgcgcctg
     4021 tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca ggtgtctgca
     4081 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc
     4141 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg
     4201 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg
     4261 gctttgggag ggggctgtcc ccgtgagctc ccaggcgcgc ctgtcattct aaatctctct
     4321 ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     4381 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     4441 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc
     4501 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg
     4561 tccccgtgag ctcaataaaa gagcccacat aacttcgtat aatgtatgct atacgaagtt
     4621 ataacgccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4681 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4741 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4801 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4861 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4921 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     4981 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     5041 gcagcgatgg gggcgggggg gggggggggg cgcgccaggc ggggcggggc ggggcgaggg
     5101 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     5161 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     5221 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
     5281 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     5341 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     5401 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     5461 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     5521 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     5581 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     5641 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     5701 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     5761 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     5821 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagtgc
     5881 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     5941 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     6001 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     6061 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     6121 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     6181 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     6241 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcgccac
     6301 tatggcccca actggggtaa cctttgagtt ctctcagttg gggggttctg gtaaaaagcc
     6361 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtgtccga
     6421 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     6481 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     6541 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaatttag
     6601 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     6661 tgaaaccgaa ctgcccgctg ttctgcaacc ggtcgcggag gccatggatg cgatcgctgc
     6721 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     6781 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     6841 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     6901 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     6961 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     7021 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     7081 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     7141 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     7201 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     7261 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     7321 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aaggatccgg
     7381 aagcggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggacc
     7441 tggatccccc aagaagaaga ggaaggtgga ggacgccgga tccgtgtcca aaggagagga
     7501 gttaatcaag gaaaacatga gaatgaaagt tgtcatggag ggctccgtta atggtcacca
     7561 attcaagtgt acaggggaag gtgaaggtaa tccttacatg ggtacacaaa ctatgagaat
     7621 taaagtaatt gaaggcggac cactaccatt tgcatttgac attctggcaa cgtcattcat
     7681 gtacggatca cgaactttca tcaagtaccc taaaggtata ccagactttt tcaagcaatc
     7741 ttttccagag ggttttacat gggaaagggt tacaagatac gaagatgggg gtgtcgtcac
     7801 agttatgcaa gatacttcat tagaagatgg ctgccttgtc tatcatgtgc aagtaagagg
     7861 ggtgaatttt ccttctaacg gacctgtgat gcagaaaaag accaaaggtt gggaaccaaa
     7921 tactgaaatg atgtacccag ctgatggagg tttgagaggc tacacacaca tggcgcttaa
     7981 agttgatggt ggaggtcatt tgtcttgtag ttttgttacc acttatcgtt ctaaaaagac
     8041 tgttggcaat atcaaaatgc caggaataca tgctgtagac cacagactag aaagactcga
     8101 agagagcgat aacgaaatgt tcgttgtaca gagagagcat gccgtagcca aatttgctgg
     8161 cttaggcggt ggtatggatg aattgtataa gggatcccat ggcttcccgc cggaggtgga
     8221 ggagcaggat gatggcgcgc tgcccatgtc ttgtgcccag gagagcggga tggaccgtca
     8281 ccctgcagcc tgtgcttctg ctaggatcaa tgtgtaactc gagtggcgat ccagacatga
     8341 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     8401 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacgct
     8461 gagca
//
