LOCUS       sequence-25184-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.58
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..112
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    191..314
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(385..402)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(385..401)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(394..416)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      465..551
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgctnnnngg agctccnaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
       61 caaattgata agcaatgctt ttttataatg ccaactttgt ataataaagt tgaaaatttg
      121 aattccggtt gaaaacccaa actactcagc cattttatta aaaatcattc aaaagtttaa
      181 aaaaattatc ccactttgta caagaaagtt gaacgagaaa cgtaaaatga tataaatatc
      241 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
      301 atgcagtcac tatgaaccaa ctacttagat ggtattagtg acctgtagaa ttcgagctct
      361 agagctgcag ggcccgagct taagactggc cgtcgtttta caacgtcgtg actgggaaaa
      421 catccatgct agcgttaacg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
      481 ctcagtcgga agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
      541 gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcancnnnnn nnnngggngn
      601 gnnngggcag gacgcccgcc ataaactgcc aggcatnnnn nnnnagcaga aggncatcct
      661 gacgganggc cttttttgcg tttctacaaa ctcttcctgg gctagcggta cgcgtattaa
      721 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      781 gaatcggcca acgcgcgggn nnngnnnttt gcgtnntnnn gctnnncng
//
