LOCUS       sequence-25185-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.57
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..172)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(350..445)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(482..501)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(483..501)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(506..522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(506..522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(624..643)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(702..721)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     gap             794..803
                     /estimated_length=10
ORIGIN
        1 tctnnagctc nnnnctacag gtcactaata ccatctaagt agttggttca tagtgactgc
       61 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      121 ttgatattta tatcatttta cgtttctcgt tcaactttct tgtacaaagt gggatgactc
      181 gtgatgatta gcttactcgt gaaatattga actatagaaa gagcaataaa gcttattcga
      241 atacttacaa tattattcta aaccaaatgc atttcaacaa tcaatatttc aatatacata
      301 taaagtcacc tttttttcta tctactcctc aatttttatt gttgatcgac aactttatta
      361 tacaaagttg gcattataaa aaagcattgc ttatcaattt gttgcaacga acaggtcact
      421 atcagtcaaa ataaaatcat tatttggagc tccatggtag cgttaacgcg gccgcgatat
      481 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
      541 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
      601 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
      661 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
      721 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg angcgccana
      781 nnnnnntnnn nnannnnnnn nnnanangt
//
