LOCUS       sequence-25186-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.56
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..172)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(237..332)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(369..388)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(370..388)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(393..409)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(393..409)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(511..530)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(589..608)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     gap             775..788
                     /estimated_length=14
ORIGIN
        1 tctngagctc gattctacag gtcactaata ccatctaagt agttggttca tagtgactgc
       61 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      121 ttgatattta tatcatttta cgtttctcgt tcaactttct tgtacaaagt gggatgagaa
      181 cttctaaata aatattgttt tacctcattg tttgttaatt tttaggatta aaataccaac
      241 tttattatac aaagttggca ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca
      301 ggtcactatc agtcaaaata aaatcattat ttggagctcc atggtagcgt taacgcggcc
      361 gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
      421 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
      481 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
      541 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
      601 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
      661 cgccagantt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
      721 tggtcagact aaactggctg acggaattta tgcctcttnc nnnnnnnaag cnttnnnnnn
      781 nnnnnnnncn gannnatgca nggnttacnn cnncnnctgn cnatccccgg aaaannngc
//
