LOCUS       sequence-25187-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.56
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..112
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    177..300
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(371..388)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(371..387)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(380..402)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      451..537
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      633..660
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgctnnnngg agctccnaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
       61 caaattgata agcaatgctt ttttataatg ccaactttgt ataataaagt tggtatttta
      121 atcctaaaaa ttaacaaaca atgaggtaaa acaatattta tttagaagtt ctcatcccac
      181 tttgtacaag aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta
      241 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatgc agtcactatg
      301 aaccaactac ttagatggta ttagtgacct gtagaattcg agctctagag ctgcagggcc
      361 cgagcttaag actggccgtc gttttacaac gtcgtgactg ggaaaacatc catgctagcg
      421 ttaacgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac
      481 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
      541 ccgggagcgg atttgaacgt tgtgaagcan cnnnngnnnn ggggngnnng ggcaggacgc
      601 ccgccataaa ctgccaggca tcnnnnnnna gcagaaggcc atcctgacgg anggcctttt
      661 tgcgtttcta caaactcttc ctggctagcg gtacgcgtat taattgcgtt gcgctcactg
      721 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt natgaatcgg ccaacgcgcg
      781 ggganangcg gtttgcgtat tgggcgctct tccgctt
//
