LOCUS       sequence-251874-        7556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses nut lipid binding TCR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7556)
  TITLE     Alcocer Lab plasmids
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMJA295
FEATURES             Location/Qualifiers
     source          1..7556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             complement(770..781)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1454..1473)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1519..1574
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1573..1592)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     2081..2100
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2175..2279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2280..2348,2349..3140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2498..2517)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3311..3899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3800..3819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4053..4070
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4187..4208
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4223..4228,4229..4246,4247..4253)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4261..4277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4266..4288
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4285..4301
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4285..4301
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4350..4471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4361..4380)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4415..4434
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4601..4975)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(4994..5041)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5089..5418)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5103..5238
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5157..5176)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     5403..5423
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(5432..5860)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5542..5563)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5754..5773
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    5906..6130
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6119..6136
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagggatc
      541 ctccctccta ctgtcttctg tgctagggat cacttccttg ttgagtgggg cctgagtttt
      601 aagaggatct tctgctcctc ttcatctggt ccctttcctt ccaaggcctc agagaggaag
      661 gcatgtgaga cgcgtattat aagaaactct ttcgtctcgg cagtggaaca acacttgttg
      721 tcacagataa acaacttgat gcagatgttt cccccaagcc cactattttt cttccttcaa
      781 ttgctgaaac aaaactccag aaggctggaa catacctttg tcttcttgag aaatttttcc
      841 cagatattat taagatacat tggcaagaaa agaagagcaa cacgattctg ggctcccagg
      901 aggggaacac catgaagact aacgacacat acatgaaatt tagctggtta acggtgccag
      961 aagagtcact ggacaaagaa cacagatgta tcgtcagaca tgagaataat aaaaacggaa
     1021 ttgatcaaga aattatcttt cctccaataa agacagatgt caccacagtg gaccccaaag
     1081 acagttattc aaaagatgca aatgatgtca tcacaatgga ccccaaagac aattggtcaa
     1141 aagatgcaaa tgatacacta atgctgcagc tcacaaacac ctctgcatat tacacgtacc
     1201 tcctccggct cctcaagagg ggggcctatt ttggccatat ccacctgctg tctgccttaa
     1261 aagaaacggg ttttcttcct gcaaatggaa aagaaaatct aaccccaacc tttcttgggt
     1321 cacaaagttg ggcattaata agaaaaaggc attgggttta ttaaggaatt caattcactc
     1381 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca
     1441 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     1501 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     1561 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     1621 agtatttggt ttagagtttg gcaacatatg ccatatgctg gctgccatga acaaaggtgg
     1681 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     1741 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     1801 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     1861 ctcccagtca tagctgtccc tcttctctta ctagtcaata atcaatgtca acgcgtatat
     1921 ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt cttcatgcaa
     1981 ttgtcggtca agccttgcct tgttgtagct taaattttgc tcgcgcacta ctcagcgacc
     2041 tccaacacac aagcagggag cagatactgg cttaactatg cggcatcaga gcagattgta
     2101 ctgagagtgc accatagggg atcgggagat ctcccgatcc gtcgacgtca ggtggcactt
     2161 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2221 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2281 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     2341 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     2401 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     2461 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     2521 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     2581 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     2641 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     2701 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     2761 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     2821 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     2881 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     2941 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     3001 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     3061 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     3121 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     3181 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3241 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3301 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3361 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3421 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3481 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3541 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3601 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3661 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3721 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3781 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3841 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3901 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3961 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4021 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4081 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4141 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     4201 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     4261 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
     4321 agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac
     4381 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4441 tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat
     4501 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
     4561 catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca
     4621 cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct
     4681 cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg
     4741 accactcggc gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg
     4801 gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg
     4861 cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg
     4921 ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt
     4981 agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa
     5041 cacgtgctga tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc
     5101 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc
     5161 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     5221 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     5281 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     5341 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     5401 acaccctaac tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc
     5461 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     5521 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     5581 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     5641 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     5701 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     5761 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     5821 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc
     5881 cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta
     5941 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga
     6001 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg
     6061 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta
     6121 gaaggcacag tcgaggctga tcagcgggtt taaacgggcc ctctagatta caagaaaaat
     6181 aacttggcag tcaagagaaa attgacggca acagtctttg caaacagcat tcgtagccca
     6241 agcactgtga gggacatcat gttcaccttc tcggtatgaa ctatggcttt gggtttatgg
     6301 cagctctttg aaggttgctt tgtgttttca gtttcctttg gttttacgtg atctgtagaa
     6361 tctgtcttca cttcaaagtc agtggagtgc acagttttat tgtcgtgttg aactgaacat
     6421 gtcactgaat ttgaatcttc atatttacca agcttgacag cattgtactt cccaccggga
     6481 gagatgacaa tagcaggatc aaactctgtt atcttcttgg atgacacgag atttattctt
     6541 atatccttgg ggtaaaattc cttcaccaga caagcgacat ttgttccatt tttcatgaca
     6601 aaaacggatg gtttggtatg aggctgactt cttggttcca cagtcacacg ggttcctttt
     6661 ccaaagatga gtttatcggt gtacgtgagg cgtatccccc agtttagggg tggaatatag
     6721 accccaagag cacaaaagta ctttgctgaa tcttctagct gtaaggctga aatggttaag
     6781 gcgacggatt tcgctgcttt cttgaagttg acagaatagc gaccactttt tgcattctgt
     6841 tcatcagaac cctggcgaat aaggaaaatc atctctttgc tgggaagttg cttgtaccaa
     6901 aaaatataat atgaccacca acttgtttca tacaggcagt tcagggtgac tgctttcctc
     6961 actggcatgg atactgatga ctgggcttga gtaaccttct gggccacact tgatcctgtg
     7021 ggaaaaatca gaaaacagag ctgatccttt gatatagtga ataaaatatc gtaagagcta
     7081 gactaattta ggacccagag acaaaccaaa ctaaaatcat aatatgctcg cttttttaca
     7141 atttctcctt tcctcctcaa gtccatgaga aataccatat gcctgtttgg aatccttcca
     7201 tccttaacat ttttactcac acctggaacc atccatacca gagtagctga aggccacaaa
     7261 tacacacagc aggctggaga acagcatcag ctgtttcttg taacattaaa cagggactct
     7321 caggaagtac agtcagccaa tcagagaaca gatgtgcaca agcacacaca cacacacaca
     7381 ggatctgggc tcccgcggca tatgaccggt ggtacctcca ggcgatctga cggttcacta
     7441 aacgagctct gcttatatag gcctcccacc gtacacgcca cctcgacata ctcgagtagt
     7501 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccg
//
