LOCUS       sequence-25190-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.51
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..112
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    232..355
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(426..443)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(426..442)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(435..457)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      506..592
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      690..717
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgctnnnngg ngctccnaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
       61 caaattgata agcaatgctt ttttataatg ccaactttgt ataataaagt tgcatttaaa
      121 gagtataaaa caacagtact agtagacatt atcaactttc atgcaataaa taggcaaaaa
      181 tagatatact ttacagacat ctgatttagt aattttgtag tagaattcat cccactttgt
      241 acaagaaagt tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
      301 tgcataaaaa acagactaca taatactgta aaacacaaca tatgcagtca ctatgaacca
      361 actacttaga tggtattagt gacctgtaga attcgagctc tagagctgca gggcccgagc
      421 ttaagactgg ccgtcgtttt acaacgtcgt gactgggaaa acatccatgc tagcgttaac
      481 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg aagactgggc
      541 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
      601 agcgganttg aacgttgtga agcaacgnnc cggnnnnngg nnnggcgggg caggacgccc
      661 gccataaact gccaggcatc annnnaagca gaangccatc ctgacggatg gcctttttgc
      721 gtttctacaa actcttcctg gctagcggta cgcgtattaa ttgcgttgcg ctcactgccc
      781 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcngcc
//
