LOCUS       sequence-251907-       12506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FLT1 W589*.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-FLT1-W589*
FEATURES             Location/Qualifiers
     source          1..12506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7466..7490)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7498..7516
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7541..7560)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7542..7560)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7566..7586)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7640..7681
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7702..8269
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7719..8271
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7863..7880)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8090..8109
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8283..8882
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8283..8302)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8779..8799
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8896..9484
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8949..8969)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9367..9378)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9559..9792
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9911..9929)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9997..10101
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10102..10170,10171..10962)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10320..10339)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11133..11721
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11622..11641
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11875..11892
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12132..12153
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12168..12173,12174..12191,12192..12198)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12206..12222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12211..12233
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12230..12246
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12230..12246
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12296..12315
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gtcagctact gggacaccgg ggtcctgctg
     3481 tgcgcgctgc tcagctgtct gcttctcaca ggatctagtt caggttcaaa attaaaagat
     3541 cctgaactga gtttaaaagg cacccagcac atcatgcaag caggccagac actgcatctc
     3601 caatgcaggg gggaagcagc ccataaatgg tctttgcctg aaatggtgag taaggaaagc
     3661 gaaaggctga gcataactaa atctgcctgt ggaagaaatg gcaaacaatt ctgcagtact
     3721 ttaaccttga acacagctca agcaaaccac actggcttct acagctgcaa atatctagct
     3781 gtacctactt caaagaagaa ggaaacagaa tctgcaatct atatatttat tagtgataca
     3841 ggtagacctt tcgtagagat gtacagtgaa atccccgaaa ttatacacat gactgaagga
     3901 agggagctcg tcattccctg ccgggttacg tcacctaaca tcactgttac tttaaaaaag
     3961 tttccacttg acactttgat ccctgatgga aaacgcataa tctgggacag tagaaagggc
     4021 ttcatcatat caaatgcaac gtacaaagaa atagggcttc tgacctgtga agcaacagtc
     4081 aatgggcatt tgtataagac aaactatctc acacatcgac aaaccaatac aatcatagat
     4141 gtccaaataa gcacaccacg cccagtcaaa ttacttagag gccatactct tgtcctcaat
     4201 tgtactgcta ccactccctt gaacacgaga gttcaaatga cctggagtta ccctgatgaa
     4261 aaaaataaga gagcttccgt aaggcgacga attgaccaaa gcaattccca tgccaacata
     4321 ttctacagtg ttcttactat tgacaaaatg cagaacaaag acaaaggact ttatacttgt
     4381 cgtgtaagga gtggaccatc attcaaatct gttaacacct cagtgcatat atatgataaa
     4441 gcattcatca ctgtgaaaca tcgaaaacag caggtgcttg aaaccgtagc tggcaagcgg
     4501 tcttaccggc tctctatgaa agtgaaggca tttccctcgc cggaagttgt atggttaaaa
     4561 gatgggttac ctgcgactga gaaatctgct cgctatttga ctcgtggcta ctcgttaatt
     4621 atcaaggacg taactgaaga ggatgcaggg aattatacaa tcttgctgag cataaaacag
     4681 tcaaatgtgt ttaaaaacct cactgccact ctaattgtca atgtgaaacc ccagatttac
     4741 gaaaaggccg tgtcatcgtt tccagacccg gctctctacc cactgggcag cagacaaatc
     4801 ctgacttgta ccgcatatgg tatccctcaa cctacaatca agtggttctg gcacccctgt
     4861 aaccataatc attccgaagc aaggtgtgac ttttgttcca ataatgaaga gtcctttatc
     4921 ctggatgctg acagcaacat gggaaacaga attgagagca tcactcagcg catggcaata
     4981 atagaaggaa agaataagat ggctagcacc ttggttgtgg ctgactctag aatttctgga
     5041 atctacattt gcatagcttc caataaagtt gggactgtgg gaagaaacat aagcttttat
     5101 atcacagatg tgccaaatgg gtttcatgtt aacttggaaa aaatgccgac ggaaggagag
     5161 gacctgaaac tgtcttgcac agttaacaag ttcttataca gagacgttac ttggatttta
     5221 ctgcggacag ttaataacag aacaatgcac tacagtatta gcaagcaaaa aatggccatc
     5281 actaaggagc actccatcac tcttaatctt accatcatga atgtttccct gcaagattca
     5341 ggcacctatg cctgcagagc caggaatgta tacacagggg aagaaatcct ccagaagaaa
     5401 gaaattacaa tcagagatca ggaagcacca tacctcctgc gaaacctcag tgatcacaca
     5461 gtggccatca gcagttccac cactttagac tgtcatgcta atggtgtccc cgagcctcag
     5521 atcacttggt ttaaaaacaa ccacaaaata caacaagagc ctggaattat tttaggacca
     5581 ggaagcagca cgctgtttat tgaaagagtc acagaagagg atgaaggtgt ctatcactgc
     5641 aaagccacca accagaaggg ctctgtggaa agttcagcat acctcactgt tcaaggaacc
     5701 tcggacaagt ctaatctgga gctgatcact ctaacatgca cctgtgtggc tgcgactctc
     5761 ttctggctcc tattaaccct ctttatccga aaaatgaaaa ggtcttcttc tgaaataaag
     5821 actgactacc tatcaattat aatggaccca gatgaagttc ctttggatga gcagtgtgag
     5881 cggctccctt atgatgccag caagtgggag tttgcccggg agagacttaa actgggcaaa
     5941 tcacttggaa gaggggcttt tggaaaagtg gttcaagcat cagcatttgg cattaagaaa
     6001 tcacctacgt gccggactgt ggctgtgaaa atgctgaaag agggggccac ggccagcgag
     6061 tacaaagctc tgatgactga gctaaaaatc ttgacccaca ttggccacca tctgaacgtg
     6121 gttaacctgc tgggagcctg caccaagcaa ggagggcctc tgatggtgat tgttgaatac
     6181 tgcaaatatg gaaatctctc caactacctc aagagcaaac gtgacttatt ttttctcaac
     6241 aaggatgcag cactacacat ggagcctaag aaagaaaaaa tggagccagg cctggaacaa
     6301 ggcaagaaac caagactaga tagcgtcacc agcagcgaaa gctttgcgag ctccggcttt
     6361 caggaagata aaagtctgag tgatgttgag gaagaggagg attctgacgg tttctacaag
     6421 gagcccatca ctatggaaga tctgatttct tacagttttc aagtggccag aggcatggag
     6481 ttcctgtctt ccagaaagtg cattcatcgg gacctggcag cgagaaacat tcttttatct
     6541 gagaacaacg tggtgaagat ttgtgatttt ggccttgccc gggatattta taagaacccc
     6601 gattatgtga gaaaaggaga tactcgactt cctctgaaat ggatggctcc tgaatctatc
     6661 tttgacaaaa tctacagcac caagagcgac gtgtggtctt acggagtatt gctgtgggaa
     6721 atcttctcct taggtgggtc tccataccca ggagtacaaa tggatgagga cttttgcagt
     6781 cgcctgaggg aaggcatgag gatgagagct cctgagtact ctactcctga aatctatcag
     6841 atcatgctgg actgctggca cagagaccca aaagaaaggc caagatttgc agaacttgtg
     6901 gaaaaactag gtgatttgct tcaagcaaat gtacaacagg atggtaaaga ctacatccca
     6961 atcaatgcca tactgacagg aaatagtggg tttacatact caactcctgc cttctctgag
     7021 gacttcttca aggaaagtat ttcagctccg aagtttaatt caggaagctc tgatgatgtc
     7081 agatacgtaa atgctttcaa gttcatgagc ctggaaagaa tcaaaacctt tgaagaactt
     7141 ttaccgaatg ccacctccat gtttgatgac taccagggcg acagcagcac tctgttggcc
     7201 tctcccatgc tgaagcgctt cacctggact gacagcaaac ccaaggcctc gctcaagatt
     7261 gacttgagag taaccagtaa aagtaaggag tcggggctgt ctgatgtcag caggcccagt
     7321 ttctgccatt ccagctgtgg gcacgtcagc gaaggcaagc gcaggttcac ctacgaccac
     7381 gctgagctgg aaaggaaaat cgcgtgctgc tccccgcccc cagactacaa ctcggtggtc
     7441 ctgtactcca ccccacccat ctagaaccca gctttcttgt acaaagtggt gatatccaat
     7501 taaccctcac taaaggttgt gaatgattaa tgaatagaag ccctatagtg agtcgtatta
     7561 ccacccaact tttctataca aagtggttga tatccagcac agtggcggcc gctcgagtct
     7621 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     7681 gcgtaccggt tagtaatgag atccctcccc cccccntaac gttactggcc gaagccgctt
     7741 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     7801 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     7861 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     7921 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     7981 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     8041 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     8101 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     8161 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     8221 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac
     8281 atatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     8341 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     8401 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     8461 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     8521 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     8581 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     8641 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     8701 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     8761 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     8821 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     8881 aaatcgatag atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     8941 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     9001 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     9061 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     9121 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     9181 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     9241 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     9301 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     9361 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     9421 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     9481 ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     9541 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     9601 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9661 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9721 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9781 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     9841 aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga
     9901 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     9961 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    10021 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    10081 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    10141 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    10201 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    10261 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    10321 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
    10381 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    10441 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    10501 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    10561 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    10621 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    10681 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    10741 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    10801 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    10861 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    10921 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    10981 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    11041 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    11101 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    11161 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    11221 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    11281 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    11341 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    11401 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    11461 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    11521 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    11581 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    11641 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    11701 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    11761 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    11821 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    11881 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    11941 ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg
    12001 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    12061 gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag
    12121 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    12181 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    12241 tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    12301 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
    12361 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
    12421 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
    12481 taaatatgta ctacaaactt agtagt
//
