LOCUS       sequence-251909-        6519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of human MEF2B with an HA  tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Han TH, Prywes R
  TITLE     Regulatory role of MEF2D in serum induction of the c-jun promoter.
  JOURNAL   Mol Cell Biol. 1995 Jun;15(6):2907-15.
  PUBMED    7760790
REFERENCE   2  (bases 1 to 6519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1995-06"; volume: "15(6)"; pages: "2907-15"
COMMENT     Sequence Label: pCGN-MEF2B
FEATURES             Location/Qualifiers
     source          1..6519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     56..78
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     70..87
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     71..87
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     94..113
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        94..112
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        131..434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        435..638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     589..609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           728..767
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             797..823
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             803..829
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     intron          2348..2920
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3052..3071)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3117..3172
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3171..3190)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      3526..3661
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3767..3784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3938..4526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4018..4037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4697..5488,5489..5557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5320..5339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5558..5662)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5730..5748
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5786..5808)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5908..5927
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5994..6449)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6131..6152)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6343..6362
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
       61 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattc
      121 gagctcgccc cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      181 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      361 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      421 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      481 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      541 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      601 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccgggggat cttggtggcg tgaaactccc gcacctcttc ggccagcgcc ttgtagaagc
      781 gcgtatggct tctagctatc cttatgacgt gcctgactat gccagcctgg gaggaccttc
      841 tagaattcga gctcggtacc ccatggggag gaaaaaaatc cagatctccc gcatcctgga
      901 ccaaaggaat cggcaggtga cgttcaccaa gcggaagttc gggctgatga agaaggccta
      961 tgagctgagc gtgctctgtg actgtgagat agccctcatc atcttcaaca gcgccaaccg
     1021 cctcttccag tatgccagca cggacatgga ccgtgtgctg ctgaagtaca cagagtacag
     1081 cgagccccac gagagccgca ccaacactga catcctcgag acgctgaagc ggaggggcat
     1141 tggcctcgat gggccagagc tggagccgga tgaagggcct gaggagccag gagagaagtt
     1201 tcggaggctg gcaggcgaag ggggtgatcc ggccttgccc cgaccccggc tgtatcctgc
     1261 agctcctgct atgcccagcc cagatgtggt atacggggcc ttaccgccac caggctgtga
     1321 ccccagtggg cttggggaag cactgcccgc ccagagccgc ccatctccct tccgaccagc
     1381 agcccccaaa gccgggcccc caggcctggt gcaccctctc ttctcaccaa gccacctcac
     1441 cagcaagaca ccacccccac tgtacctgcc gacggaaggg cggaggtcag acctgcctgg
     1501 tggcctggct gggccccgag ggggactaaa cacctccaga agcctctaca gtggcctgca
     1561 gaacccctgc tccactgcaa ctcccggacc cccactgggg agcttcccct tcctccccgg
     1621 aggcccccca gtgggggccg aagcctgggc gaggagggtc ccccaacccg cggcgcctcc
     1681 ccgccgaccc ccccagtcag catcaagtct gagcgcctct ctccggcccc cgggggcccc
     1741 ggcgactttc ctaagacctt cccctatccc ttgctcctcg cccggtccct ggcagagcct
     1801 ctgcggcctg ggcccgccct gcgccggctg cccttggccg acggctggcc ccggtaggag
     1861 atcacccggt ggcaccagcc cagagcgctc gccaggtacg gcgagggcac gtggggaccc
     1921 cacctccctc caggcctctt cagagaagac ccaacagtga cgcccccctc cgcggtgggg
     1981 gcttggaggt gggcggctgg actcaatcca ccctgggggg ctcctttcct tcttcctatt
     2041 tgtgtgtata tccacaaata aaacgcgcgt ggcgtccgtg gaccaaaaaa aaaaaaaaaa
     2101 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2161 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaraarrmwm amawaaaaaa
     2221 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2281 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa acccgaattc ggatccgcgg atcctgagaa
     2341 cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat
     2401 gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta
     2461 tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt
     2521 gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt
     2581 gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc
     2641 actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac
     2701 aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg
     2761 aaatattctt attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg
     2821 gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc
     2881 ctctgctaac catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt
     2941 tgttgtgctg tctcatcatt ttggcaaaga attcactcct caggtgcagg ctgcctatca
     3001 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     3061 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     3121 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     3181 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     3241 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     3301 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     3361 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     3421 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     3481 tcttctctta tggagatccc tcgaggagct ttttgcaaaa gcctaggcct ccaaaaaagc
     3541 ctcttcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     3601 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     3661 tgggcggagt taggggcggg actatggttg ctgactaatt gctgcattaa tgaatcggcc
     3721 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     3781 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3841 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3901 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3961 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4021 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4081 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4141 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4201 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4261 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4321 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4381 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4441 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4501 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4561 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4621 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4681 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4741 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4801 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4861 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4921 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4981 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5041 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5101 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5161 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5221 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5281 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5341 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5401 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5461 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5521 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5581 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5641 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     5701 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     5761 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     5821 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     5881 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     5941 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
     6001 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     6061 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     6121 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     6181 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     6241 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     6301 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     6361 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     6421 cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg
     6481 gaagggcgat cggtgcgggc ctcttcgcta ttacgccag
//
