LOCUS       sequence-251910-        3794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  asymmetric plasmid partitioning E. coli - negative control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pCC2
FEATURES             Location/Qualifiers
     source          1..3794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             191..865
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1048..1075
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             1507..1698
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1692..1714)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1800..1939
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1855..1874
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1954..1971)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2125..2713)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2205..2224)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2762..3421)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3336..3355
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(3735..3756)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtttgatgac aaaaaattag cgcaagaaga caaaaatcac cttgcgctaa gagctcgaca
       61 ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa gcatgccact
      121 taaaaaggag atcaacaatg aaagcaattt tcgtactgaa acatcttaat catgctgggg
      181 agggtttcta atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg
      241 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc
      301 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc
      361 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc
      421 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat
      481 gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga
      541 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca
      601 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct
      661 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt
      721 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat
      781 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc
      841 tgaaggtcgt cactccaccg gtgctacctc cgcagcgaac gacgaaaatt acgcccttgc
      901 agcgtaataa ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc aaccggtgat
      961 accacgatac tatgactgag agtcaacgcc atgagcggcc tcatttctta ttctgagtta
     1021 caacagtccg caccgctgtc tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1081 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tatttcctag
     1141 ctagccaacg catatggcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt
     1201 ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg
     1261 atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag
     1321 cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt
     1381 ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc
     1441 atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacggag
     1501 gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag
     1561 acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt
     1621 gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat
     1681 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     1741 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     1801 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     1861 cagagcagat tgtactgaga gtgcaccatt gcggtgtgaa ataccgcaca gatgcgtaag
     1921 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1981 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2041 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2101 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2161 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2221 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2281 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2341 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2401 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2461 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2521 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     2581 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2641 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2701 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacagct tggtctgaca
     2761 gttacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2821 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2881 cgccttgcgt ataatatttg cccatcgtga aaacgggggc gaagaagttg tccatattgg
     2941 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     3001 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     3061 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     3121 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     3181 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     3241 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     3301 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     3361 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     3421 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     3481 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactta ccaatgctgt
     3541 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     3601 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     3661 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     3721 ggttgagtgt tgttccagtt tggaacaaga gtccacctcg agctttcagg aaaatttttc
     3781 tgtataatag attc
//
