LOCUS       sequence-251915-        4433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  asymmetric plasmid partitioning E. coli - target plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pCC28
FEATURES             Location/Qualifiers
     source          1..4433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..474
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             740..1414
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1597..1624
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2056..2247
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2241..2263)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2349..2488
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2404..2423
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2503..2520)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2674..3262)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2754..2773)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3311..3970)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3885..3904
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(4284..4305)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcctgaata ttctctctgg gcccactgtt ccacttgtat cgtcggtctg ataatcagac
       61 tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc acggtcccac
      121 tcgtatcgtc ggtctgatta ttagtctggg accacggtcc cactcgtatc gtcggtctga
      181 taatcagact gggaccacgg tcccactcgt atcgtcggtc tgattattag tctgggacca
      241 tggtcccact cgtatcgtcg gtctgattat tagtctggga ccacggtccc actcgtatcg
      301 tcggtctgat tattagtctg gaaccacggt cccactcgta tcgtcggtct gattattagt
      361 ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacgatccca
      421 ctcgtgttgt cggtctgatt atcggtctgg gaccacggtc ccacttgtat tgtcgatcag
      481 actatcagcg tgagactacg attccatcaa tgcctgtcaa gggcaagtat tgacatgtcg
      541 tcgtaacctg tagaacggag taacctcggt gcaagaagac aaaaatcacc ttgcgctaag
      601 agctcgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag
      661 catgccactt aaaaaggaga tcaacaatga aagcaatttt cgtactgaaa catcttaatc
      721 atgctgggga gggtttctaa tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt
      781 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
      841 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
      901 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
      961 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
     1021 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
     1081 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
     1141 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga
     1201 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
     1261 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa
     1321 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
     1381 cgaacgtgct gaaggtcgtc actccaccgg tgctacctcc gcagcgaacg acgaaaatta
     1441 cgcccttgca gcgtaataac tacgtgctgc tgaagttgcc cgcaacagag agtggaacca
     1501 accggtgata ccacgatact atgactgaga gtcaacgcca tgagcggcct catttcttat
     1561 tctgagttac aacagtccgc accgctgtct cacactggct caccttcggg tgggcctttc
     1621 tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg cttttttatt
     1681 atttcctagc tagccaacgc atatggcaaa acgtctgcga cctgagcaac aacatgaatg
     1741 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt
     1801 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
     1861 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
     1921 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
     1981 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct
     2041 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc
     2101 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca
     2161 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
     2221 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     2281 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     2341 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     2401 atgcggcatc agagcagatt gtactgagag tgcaccattg cggtgtgaaa taccgcacag
     2461 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     2521 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2581 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2641 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2701 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2761 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2821 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2881 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2941 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3001 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3061 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     3121 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3181 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3241 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacagctt
     3301 ggtctgacag ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     3361 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     3421 gcaccttgtc gccttgcgta taatatttgc ccatcgtgaa aacgggggcg aagaagttgt
     3481 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     3541 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     3601 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     3661 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     3721 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
     3781 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     3841 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     3901 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     3961 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     4021 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaacttac
     4081 caatgctgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4141 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     4201 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     4261 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccacctcga gctttcagga
     4321 aaatttttct gtataataga ttcataaatt tgagagaaga gtgcgaccac gttttagtct
     4381 acgtttatct gtctttactt aatgtccttt gttacaggcc agaaagcata act
//
