LOCUS       sequence-251922-        8666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TIP-LITer2.0-KRAS(G12V) gene circuit with the following 
            architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-KRAS(G12V)-P2AGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Guinn MT, Balazsi G
  TITLE     Noise-reducing optogenetic negative-feedback gene circuits in human 
            cells.
  JOURNAL   Nucleic Acids Res. 2019 Jul 3. pii: 5527975. doi: 
            10.1093/nar/gkz556.
  PUBMED    31269201
REFERENCE   2  (bases 1 to 8666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2019 Jul 3. pii: 5527975. doi: 10.1093/nar/gkz556."
COMMENT     Sequence Label: pMTG8-Amp-FRT
FEATURES             Location/Qualifiers
     source          1..8666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    580..598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    606..624
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          807..1379
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1391..1400
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2537..2593
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(2594..2626,2627..2629,2630..3157)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase K-Ras"
                     /label=K-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     CDS             3167..3223
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3224..3226,3227..3229,3230..3943)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3224..3226,3227..3229,3230..3940)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3269..3290)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3530..3549)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3877..3898
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4011..4031)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(4384..4401)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4390..4614
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4747..4766)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4898..4945
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4953..5975
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4953..5975
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6105..6226
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6142..6161)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6196..6215
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6275..6291)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6275..6291)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6288..6310)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6299..6315
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6323..6329,6330..6347,6348..6353))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6368..6389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6506..6523)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6677..7265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6757..6776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7436..8227,8228..8296))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8059..8078
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8297..8401)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8476..8495)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcat ccctatcagt gatagagatc
      601 agatctccct atcagtgata gagagctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gactctagcg tttaaactta agcttggtac ccggggatcc tctagggcct
      721 ctgagctatt ccagaagtag tgaagaggct tttttggagg cctaggcttt tgcaaaaagc
      781 tccggatcga tcctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt
      841 tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa
      901 tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc
      961 tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt
     1021 taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt
     1081 gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt
     1141 cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata
     1201 taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacatc ctggtcatca
     1261 tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact
     1321 ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttcttt ttcctacagg
     1381 tcctgcaggc gccaccatgg gttctagact ggacaagagc aaagtcataa actctgctct
     1441 ggaattactc aatgaagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
     1501 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
     1561 cctggcaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
     1621 atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc tctcacatcg
     1681 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
     1741 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
     1801 gtccgccgtg ggccacttta cactgggctg cgtattggag gatcaggagc atcaagtagc
     1861 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
     1921 tgagctgttc gaccatcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
     1981 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccaaaaaaga agagaaaggc
     2041 tagcggcgcc ggcgcctttt tggctactac acttgaacgt attgagaaga actttgtcat
     2101 tactgaccca aggttgccag ataatcccat tatattcgcg tccgatagtt tcttgcagtt
     2161 gacagaatat agccgtgaag aaattttggg aagaaactgc aggtttctac aaggtcctga
     2221 aactgatcgc gcgacagtga gaaaaattag agatgccata gataaccaaa cagaggtcac
     2281 tgttcagctg attaattata caaagagtgg taaaaagttc tggaacctct ttcacttgca
     2341 gcctatgcga gatcagaagg gagatgtcca gtactttatt ggggttcagt tggatggaac
     2401 tgagcatgtc cgagatgctg ccgagagaga gggcgtgatg ctggccaaga agaccgccga
     2461 gaacatcgac gaggccgcct ggacctggaa cgcctacgcc ttcgccgccc cctccggcgg
     2521 cggctccgga agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga
     2581 gaaccctgga cctatgactg aatataaact tgtggtagtt ggagctgttg gcgtaggcaa
     2641 gagtgccttg acgatacagc taattcagaa tcattttgtg gacgaatatg atccaacaat
     2701 agaggattcc tacaggaagc aagtagtaat tgatggagaa acctgtctct tggatattct
     2761 cgacacagca ggtcaagagg agtacagtgc aatgagggac cagtacatga ggactgggga
     2821 gggctttctt tgtgtatttg ccataaataa tactaaatca tttgaagata ttcaccatta
     2881 tagagaacaa attaaaagag ttaaggactc tgaagatgta cctatggtcc tagtaggaaa
     2941 taaatgtgat ttgccttcca gaacagtaga cacaaaacag gctcaggact tagcaagaag
     3001 ttatggaatt ccttttattg aaacatcagc aaagacaaga cagggtgttg atgatgcctt
     3061 ctatacatta gttcgagaaa ttcgaaaaca taaagaaaag atgagcaaag atggtaaaaa
     3121 gaagaaaaag aagtcaaaga caaagtgtgt aattatggga agcggagcta ctaacttcag
     3181 cctgctgaag caggctggag acgtggagga gaaccctgga cctatggtga gcaagggcga
     3241 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3301 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3361 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3421 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3481 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3541 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3601 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3661 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3721 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3781 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3841 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3901 cgccgccggg atcactctcg gcatggacga gctgtacaag taaaccggtg cggccgcaat
     3961 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     4021 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     4081 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4141 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4201 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4261 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4321 ggcactgaca attccgtggt gttgtcctcg agtctagagg gcccgtttaa acccgctgat
     4381 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     4441 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     4501 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4561 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     4621 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     4681 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4741 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4801 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4861 ccaaaaaact tgattagggt gatggttcac gtacctagaa gttcctattc cgaagttcct
     4921 attctctaga aagtatagga acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg
     4981 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     5041 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     5101 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     5161 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     5221 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     5281 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     5341 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     5401 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     5461 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     5521 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     5581 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     5641 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     5701 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     5761 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     5821 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     5881 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     5941 gacgccccag cactcgtccg agggcaaagg aatagcacgt actacgagat ttcgattcca
     6001 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     6061 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     6121 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6181 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     6241 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6301 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6361 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6421 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6481 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6541 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6601 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6661 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6841 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7021 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7081 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7141 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7201 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7261 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7321 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7381 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7441 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7501 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7561 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7621 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7681 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7741 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7801 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7861 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7921 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7981 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8041 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8101 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8161 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8221 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8281 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8341 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8401 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     8461 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     8521 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     8581 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     8641 cgatgtacgg gccagatata cgcgtt
//
