LOCUS       sequence-251924-        7749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  asymmetric plasmid partitioning E. coli - target plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 3376 to 3410)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pCC48
FEATURES             Location/Qualifiers
     source          1..7749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             116..289
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGE"
     primer_bind     complement(136..153)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(136..152)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(145..167)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(3376..3410)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             4128..4802
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTAYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      4985..5012
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             5444..5635
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5629..5651)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5737..5876
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5792..5811
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5891..5908)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6062..6650)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6142..6161)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6699..7358)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7273..7292
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(7672..7693)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 actcatcggc gtttcacgtg aaacaccccc accgcagctg tgagcggcct gtggacaata
       61 ttggggatgt tccacgtgaa acatcacttg gtttgatgac aaaaaattag cgcaaatgac
      121 catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      181 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      241 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc
      301 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc
      361 cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac
      421 caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg
      481 ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat
      541 tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta
      601 cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga
      661 aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc agttatctgg aagatcagga
      721 tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca
      781 aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga
      841 ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg
      901 gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga
      961 gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg
     1021 gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac
     1081 gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct
     1141 gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc
     1201 tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca
     1261 gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct
     1321 gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt
     1381 gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac
     1441 gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga
     1501 atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc
     1561 ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc
     1621 gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa
     1681 aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc
     1741 gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg
     1801 tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa
     1861 cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt
     1921 ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa
     1981 acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga
     2041 atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa
     2101 gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga
     2161 actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt
     2221 gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg
     2281 tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac
     2341 caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca
     2401 gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg
     2461 cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat
     2521 tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc
     2581 gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc
     2641 gtggcagcat caggggaaaa ccttatttat cagccgaaag tattcattat ggtgaagaca
     2701 cttattatca tgcagctaag gcgtagttct catcgtttgc cgcggaggta cgctgtgtac
     2761 ccggacaaac accattaatc agcaggaagg taaattcttc aatatcctgt tcaacggtca
     2821 gctgttcggt cagcaggcga taccaacaaa aaccaaaaat catatccagc agcagttcac
     2881 gattggtatc tttcggcagt tcaccattgc taatggcatt ttcaaccagt tttttcggca
     2941 tctcacgacg acgttccata aactgatctt tcagctgggt cagggttgca gggtccagct
     3001 gtgcttctgc aataacacaa cgaaatgctt caccacaaat ggtttcacgc caaactttcc
     3061 acagattacg cagcagaaaa tccagatcgg ctttaaagct acccagatcc ggaaatttac
     3121 gcacctgttc gctttcattt tcatacactt cggcaatcag tgctgcttta ttggtccacc
     3181 aacgataaat ggtcggtttg cttgcaccgg cacgacgtgc aacgctttca atgctcagac
     3241 cgctataacc acattctttc aggatttcaa tggtgctggt cagaattgct ttatgggtat
     3301 gcggactacg caggctacca atgctgctac ggctcggggt acgtgccatg ttatttacct
     3361 ctttacggtg tgattgctag cactgtacct aggactgagc tagccgtcaa ggtaccgata
     3421 agctgtcaaa catgagcaga tcctctacga aaacctaccg gattgatggt agtggtcaaa
     3481 tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc
     3541 tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag
     3601 aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag
     3661 acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat
     3721 tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc
     3781 aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc
     3841 tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat
     3901 cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat
     3961 aagaagacaa aaatcacctt gcgctaagag ctcgacacca tcgaatggtg caaaaccttt
     4021 cgcggtatgg catgatagcg cccggaagca tgccacttaa aaaggagatc aacaatgaaa
     4081 gcaattttcg tactgaaaca tcttaatcat gctggggagg gtttctaatg gcttcctccg
     4141 aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc
     4201 acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta
     4261 aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt
     4321 tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac
     4381 tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg
     4441 ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc
     4501 gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag
     4561 cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc
     4621 tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccgcctac atggctaaaa
     4681 aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca
     4741 acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg
     4801 ctacctccgc agcgaacgac gaaaattacg cccttgcagc gtaataacta cgtgctgctg
     4861 aagttgcccg caacagagag tggaaccaac cggtgatacc acgatactat gactgagagt
     4921 caacgccatg agcggcctca tttcttattc tgagttacaa cagtccgcac cgctgtctca
     4981 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa
     5041 gccagattat taatccggct tttttattat ttcctagcta gccaacgcat atggcaaaac
     5101 gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga
     5161 aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg
     5221 gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat
     5281 ttttctctgg tcccgccgca tccataccgc cagttgttta ccatcacaac gttccagtaa
     5341 ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat
     5401 cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa
     5461 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact
     5521 caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga
     5581 tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     5641 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     5701 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     5761 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     5821 caccattgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     5881 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5941 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6001 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6061 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6481 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     6541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6661 tgatcttttc tacggggtct gacagcttgg tctgacagtt acgccccgcc ctgccactca
     6721 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     6781 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     6841 atcgtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     6901 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
     6961 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     7021 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     7081 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
     7141 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     7201 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     7261 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     7321 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     7381 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     7441 gaacctctta cgtgcccgat caacttacca atgctgtatt tagaaaaata aacaaatagg
     7501 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     7561 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     7621 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     7681 aacaagagtc cacctcgagc tttcaggaaa atttttctgt ataatagatt cataaatttg
     7741 agagaagag
//
