LOCUS       sequence-25193-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.46
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..172)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(374..469)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(506..525)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(507..525)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(530..546)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(530..546)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(648..667)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     gap             769..783
                     /estimated_length=15
ORIGIN
        1 tctngagctc gnttctacag gtcactaata ccatctaagt agttggttca tagtgactgc
       61 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      121 ttgatattta tatcatttta cgtttctcgt tcaactttct tgtacaaagt gggataagcc
      181 ttcaaatttc aacgttttat tggttgtata ttttgaatat gtactgatta cgtattcaca
      241 agttttaggt tctctttttt ctaaatgatg taaaatttcc caaatcatgt tttatttatt
      301 gaaccccgtt ttattgtgat ccaagtaaat gtttgtactt ctataggaat cagatgaatt
      361 tcgctttcag actcaacttt attatacaaa gttggcatta taaaaaagca ttgcttatca
      421 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg gagctccatg
      481 gtagcgttaa cgcggccgcg atatccccta tagtgagtcg tattacatgg tcatagctgt
      541 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
      601 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
      661 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg ntgctgantt
      721 atatgggtat aaatgggctc gcgannnnnn nngggnnnnn ggnnnnnann nnnnnnnnnn
      781 nnntgnnngc ccgatgccnc cnnaanttgn ttnntgaaan nntggnnaag gtagcnnnnn
      841 nc
//
