LOCUS       sequence-251941-        6977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  asymmetric plasmid partitioning E. coli - target plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pAA41
FEATURES             Location/Qualifiers
     source          1..6977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             116..3190
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(136..153)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(136..152)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(145..167)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             3356..4030
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      4213..4240
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             4672..4863
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(4857..4879)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4965..5104
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5020..5039
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5119..5136)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5290..5878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5370..5389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5927..6586)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6501..6520
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(6900..6921)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 actcatcggc gtttcacgtg aaacaccccc accgcagctg tgagcggcct gtggacaata
       61 ttggggatgt tccacgtgaa acatcacttg gtttgatgac aaaaaattag cgcaaatgac
      121 catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      181 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      241 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc
      301 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc
      361 cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac
      421 caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg
      481 ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat
      541 tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta
      601 cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga
      661 aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc agttatctgg aagatcagga
      721 tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca
      781 aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga
      841 ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg
      901 gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga
      961 gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg
     1021 gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac
     1081 gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct
     1141 gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc
     1201 tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca
     1261 gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct
     1321 gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt
     1381 gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac
     1441 gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga
     1501 atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc
     1561 ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc
     1621 gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa
     1681 aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc
     1741 gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg
     1801 tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa
     1861 cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt
     1921 ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa
     1981 acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga
     2041 atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa
     2101 gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga
     2161 actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt
     2221 gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg
     2281 tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac
     2341 caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca
     2401 gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg
     2461 cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat
     2521 tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc
     2581 gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc
     2641 gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag
     2701 tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc atccggcgcg
     2761 gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc tcggattagg
     2821 gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc
     2881 attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac
     2941 gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg
     3001 ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg
     3061 cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc
     3121 gtcagtatcg gcggaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg
     3181 tcaaaaataa gaagacaaaa atcaccttgc gctaagagct cgacaccatc gaatggtgca
     3241 aaacctttcg cggtatggca tgatagcgcc cggaagcatg ccacttaaaa aggagatcaa
     3301 caatgaaagc aattttcgta ctgaaacatc ttaatcatgc tggggagggt ttctaatggc
     3361 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
     3421 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
     3481 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
     3541 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
     3601 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
     3661 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
     3721 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
     3781 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
     3841 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
     3901 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
     3961 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
     4021 caccggtgct acctccgcag cgaacgacga aaattacgcc cttgcagcgt aataactacg
     4081 tgctgctgaa gttgcccgca acagagagtg gaaccaaccg gtgataccac gatactatga
     4141 ctgagagtca acgccatgag cggcctcatt tcttattctg agttacaaca gtccgcaccg
     4201 ctgtctcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa
     4261 tataaaaagc cagattatta atccggcttt tttattattt cctagctagc caacgcatat
     4321 ggcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
     4381 agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga
     4441 tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc
     4501 tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt
     4561 tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc
     4621 atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa
     4681 caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg
     4741 gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac
     4801 cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     4861 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     4921 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     4981 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     5041 tgagagtgca ccattgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     5101 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5161 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5221 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5281 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5341 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5401 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5461 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5521 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5581 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5641 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5701 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     5761 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5821 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5881 agatcctttg atcttttcta cggggtctga cagcttggtc tgacagttac gccccgccct
     5941 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     6001 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     6061 atttgcccat cgtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     6121 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     6181 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     6241 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     6301 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     6361 ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     6421 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     6481 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     6541 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     6601 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     6661 gaaagttgga acctcttacg tgcccgatca acttaccaat gctgtattta gaaaaataaa
     6721 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     6781 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     6841 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     6901 cagtttggaa caagagtcca cctcgagctt tcaggaaaat ttttctgtat aatagattca
     6961 taaatttgag agaagag
//
