LOCUS       sequence-251946-        3948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Meyer C, Garzia A, Morozov P, Molina H, Tuschl T
  TITLE     The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 
            40S Subunits of Ribosomes Stalled in Translation from Lysosomal 
            Degradation.
  JOURNAL   Mol Cell. 2020 Mar 19;77(6):1193-1205.e5. doi: 
            10.1016/j.molcel.2019.12.024. Epub 2020 Jan 24.
  PUBMED    31981475
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.12.024"; journalName: "Mol Cell"; date: 
            "2020-03-19- 19"; volume: "77"; issue: "6"; pages: "1193-1205.e5"
COMMENT     Sequence Label: pENTR4_G3BP1
FEATURES             Location/Qualifiers
     source          1..3948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(127..154)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(246..332)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     381..403
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     395..412
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     396..412
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    428..527
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1932..2031)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2048..2067)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2049..2067)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2072..2088)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2072..2088)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2190..2209)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2201..3010
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2268..2287)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3157..3745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3646..3665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3899..3916
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
       61 agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga
      121 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
      181 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
      241 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
      301 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
      361 aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct
      421 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
      481 tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggcttca tggtgatgga
      541 gaagcctagt cccctgctgg tcgggcggga atttgtgaga cagtattaca cactgctgaa
      601 ccaggcccca gacatgctgc atagatttta tggaaagaac tcttcttatg tccatggggg
      661 attggattca aatggaaagc cagcagatgc agtctacgga cagaaagaaa tccacaggaa
      721 agtgatgtca caaaacttca ccaactgcca caccaagatt cgccatgttg atgctcatgc
      781 cacgctaaat gatggtgtgg tagtccaggt gatggggctt ctctctaaca acaaccaggc
      841 tttgaggaga ttcatgcaaa cgtttgtcct tgctcctgag gggtctgttg caaataaatt
      901 ctatgttcac aatgatatct tcagatacca agatgaggtc tttggtgggt ttgtcactga
      961 gcctcaggag gagtctgaag aagaagtaga ggaacctgaa gaaagacagc aaacacctga
     1021 ggtggtacct gatgattctg gaactttcta tgatcaggca gttgtcagta atgacatgga
     1081 agaacattta gaggagcctg ttgctgaacc agagcctgat cctgaaccag aaccagaaca
     1141 agaacctgta tctgaaatcc aagaggaaaa gcctgagcca gtattagaag aaactgcccc
     1201 tgaggatgct cagaagagtt cttctccagc acctgcagac atagctcaga cagtacagga
     1261 agacttgagg acattttctt gggcatctgt gaccagtaag aatcttccac ccagtggagc
     1321 tgttccagtt actgggatac cacctcatgt tgttaaagta ccagcttcac agccccgtcc
     1381 agagtctaag cctgaatctc agattccacc acaaagacct cagcgggatc aaagagtgcg
     1441 agaacaacga ataaatattc ctccccaaag gggacccaga ccaatccgtg aggctggtga
     1501 gcaaggtgac attgaacccc gaagaatggt gagacaccct gacagtcacc aactcttcat
     1561 tggcaacctg cctcatgaag tggacaaatc agagcttaaa gatttctttc aaagttatgg
     1621 aaacgtggtg gagttgcgca ttaacagtgg tgggaaatta cccaattttg gttttgttgt
     1681 gtttgatgat tctgagcctg ttcagaaagt ccttagcaac aggcccatca tgttcagagg
     1741 tgaggtccgt ctgaatgtcg aagagaagaa gactcgagct gccagggaag gcgaccgacg
     1801 agataatcgc cttcggggac ctggaggccc tcgaggtggg ctgggtggtg gaatgagagg
     1861 ccctccccgt ggaggcatgg tgcagaaacc aggatttgga gtgggaaggg ggcttgcgcc
     1921 acggcagtga gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     1981 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     2041 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2101 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     2161 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     2221 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     2281 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     2341 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     2401 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     2461 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     2521 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2581 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2641 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     2701 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     2761 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2821 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2881 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2941 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     3001 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     3061 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     3121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3781 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3841 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3901 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcc
//
