LOCUS       sequence-25195-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.44
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..80
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    262..385
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(456..473)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(456..472)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(465..487)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      536..622
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttnnctgana gtgacctgtt cgttgcaaca aattgataag caatgctttt ttataatgcc
       61 aactttgtat aataaagttg cggactgtct aaaattagtt ttgaattgat aaaacgaaca
      121 aactttatta cgaaaaaatg cgattataaa aaggaacaaa tgtcagtttg agtatctagt
      181 gagaaatgcg gtgctttgaa aaggatgaat attgtgaaaa caaattaggc gattaaacca
      241 ggtggtgata tgatttttat cccactttgt acaagaaagt tgaacgagaa acgtaaaatg
      301 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
      361 aaacacaaca tatgcagtca ctatgaacca actacttaga tggtattagt gacctgtaga
      421 attcgagctc tagagctgca gggcccgagc ttaagactgg ccgtcgtttt acaacgtcgt
      481 gactgggaaa acatccatgc tagcgttaac gcgagagtag ggaactgcca ggcatcaaat
      541 aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      601 cgctctcctg agtaggacaa atccgccggg agcgganttg aacgttgtga agcaacgnnc
      661 cggannnggg ngggcngggc aggacgcccg ccatnnctgc caggcatcaa ncttaaagca
      721 gaangncatc ctgacggatg gnctttttgc gtttctacna actcttcctg g
//
