LOCUS       sequence-251951-        8820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human SUZ12 with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8820)
  AUTHORS   Pasini D, Bracken AP, Jensen MR, Lazzerini Denchi E, Helin K
  TITLE     Suz12 is essential for mouse development and for EZH2 histone 
            methyltransferase activity.
  JOURNAL   EMBO J. 2004 Oct 13. 23(20):4061-71.
  PUBMED    15385962
REFERENCE   2  (bases 1 to 8820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2004 Oct 13. 23(20):4061-71."
COMMENT     Sequence Label: pCMVHA SUZ12
FEATURES             Location/Qualifiers
     source          1..8820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        407..610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     561..581
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     607..631
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          722..1294
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             1374..1400
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1412..1423)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3768..3787)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3833..3888
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3887..3906)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4246..4262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4246..4262)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4259..4281)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4270..4286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4294..4300,4301..4318,4319..4324))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4339..4360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4477..4494)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4648..5236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4728..4747)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5407..6198,6199..6267))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6030..6049
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6268..6372)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6440..6458
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6496..6518)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6618..6637
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6831..6853
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6845..6862
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6846..6862
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7273..7418
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             7475..8269
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7529..7548)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8139..8158
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8463..8482)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8507..8554
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      661 cctccataga agacaccggg accgatccag cctccggtcg accgatcctg agaacttcag
      721 ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata
      781 tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca
      841 tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac cattgtctcc
      901 tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg
      961 aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct aatcactttt
     1021 ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga gaacaattgt
     1081 tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc gtggaaatat
     1141 tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt tatggttaca
     1201 atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg gcccctctgc
     1261 taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc tggttgttgt
     1321 gctgtctcat cattttggca aagaattcct cgacggatca attcaccatg gcatacccat
     1381 acgatgttcc agattacgct agcggatcaa ttcgcccttc gatggcgcct cagaagcacg
     1441 gcggtggggg agggggcggc tcggggccca gcgcggggtc cgggggaggc ggcttcgggg
     1501 gttcggcggc ggtggcggcg gcgacggctt cgggcggcaa atccggcggc gggagctgtg
     1561 gagggggtgg cagttactcg gcctcctcct cctcctccgc ggcggcagcg gcgggggctg
     1621 cggtgttacc ggtgaagaag ccgaaaatgg agcacgtcca ggctgaccac gagcttttcc
     1681 tccaggcctt tgagaagcca acacagatct atagatttct tcgaactcgg aatctcatag
     1741 caccaatatt tttgcacaga actcttactt acatgtctca tcgaaactcc agaacaaaca
     1801 tcaaaaggaa aacatttaaa gttgatgata tgttatcaaa agtagagaaa atgaaaggag
     1861 agcaagaatc tcatagcttg tcagctcatt tgcagcttac gtttactggt ttcttccaca
     1921 aaaatgataa gccatcacca aactcagaaa atgaacaaaa ttctgttacc ctggaagtcc
     1981 tgcttgtgaa agtttgccac aaaaaaagaa aggatgtaag ttgtccaata aggcaagttc
     2041 ccacaggtaa aaagcaggtg cctttgaatc ctgacctcaa tcaaacaaaa cccggaaatt
     2101 tcccgtccct tgcagtttcc agtaatgaat ttgaacctag taacagccat atggtgaagt
     2161 cttactcgtt gctatttaga gtgactcgtc caggaagaag agagtttaat ggaatgatta
     2221 atggagaaac caatgaaaat attgatgtca atgaagagct tccagccaga agaaaacgaa
     2281 atcgtgagga tggggaaaag acatttgttg cacaaatgac agtatttgat aaaaacaggc
     2341 gcttacagct tttagatggg gaatatgaag tagccatgca ggaaatggaa gaatgtccaa
     2401 taagcaagaa aagagcaaca tgggagacta ttcttgatgg gaagaggctg cctccattcg
     2461 aaacattttc tcagggacct acgttgcagt tcactcttcg ttggacagga gagaccaatg
     2521 ataaatctac ggctcctatt gccaaacctc ttgccactag aaattcagag agtctccatc
     2581 aggaaaacaa gcctggttca gttaaaccta ctcaaactat tgctgttaaa gaatcattga
     2641 ctacagatct acaaacaaga aaagaaaagg atactccaaa tgaaaaccga caaaaattaa
     2701 gaatatttta tcagtttctc tataacaaca atacaaggca acaaactgaa gcaagagatg
     2761 acctgcattg cccttggtgt actctgaact gccgcaaact ttatagttta ctcaagcatc
     2821 ttaaactctg ccatagcaga tttatcttca actatgttta tcatccaaaa ggtgctagga
     2881 tagatgtttc tatcaatgag tgttatgatg gctcctatgc aggaaatcct caggatattc
     2941 atcgccaacc tggatttgct tttagtcgca acggaccagt taagagaaca cctatcacac
     3001 atattcttgt gtgcaggcca aaacgaacaa aagcaagcat gtctgaattt cttgaatctg
     3061 aagatgggga agtagaacag caaagaacat atagtagtgg ccacaatcgt ctgtatttcc
     3121 atagtgatac ctgcttacct ctccgtccac aagaaatgga agtagatagt gaagatgaaa
     3181 aggatcctga atggctaaga gaaaaaacca ttacacaaat tgaagagttt tctgatgtta
     3241 atgaaggaga gaaagaagtg atgaaactct ggaatctcca tgtcatgaag catgggttta
     3301 ttgctgacaa tcaaatgaat catgcctgta tgctgtttgt agaaaattat ggacagaaaa
     3361 taattaagaa gaatttatgt cgaaacttca tgcttcatct agtcagcatg catgacttta
     3421 atcttattag cataatgtca atagataaag ctgttaccaa gctccgtgaa atgcagcaaa
     3481 aattagaaaa gggggaatct gcttcccctg caaacgaaga aataactgaa gaacaaaatg
     3541 ggacagcaaa tggatttagt gaaattaact caaaagagaa agctttggaa acagatagtg
     3601 tctcaggggt ttcaaaacag agcaaaaaac aaaaactctg aaaagctcta accccatgtt
     3661 atggacccga attgatccgt cgaggaattc actcctcagg tgcaggctgc ctatcagaag
     3721 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
     3781 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     3841 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     3901 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     3961 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     4021 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     4081 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     4141 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     4201 ctcttatgga gatccctcga cggatcggcc gcaattcgta atcatgtcat agctgtttcc
     4261 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4321 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4381 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4441 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4501 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4561 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4621 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4681 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4741 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4801 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4861 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4921 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4981 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5041 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5101 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5161 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5221 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5281 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5341 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5401 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5461 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5521 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     5581 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     5641 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     5701 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     5761 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     5821 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5881 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5941 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6001 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6061 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6121 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6181 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6241 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6301 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6361 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     6421 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     6481 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     6541 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     6601 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     6661 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     6721 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     6781 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     6841 acgttgtaaa acgacggcca gtgccaagct tcacgctgcc gcaagcactc agggcgcaag
     6901 ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg
     6961 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag
     7021 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc
     7081 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac
     7141 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc ctgcttcatc
     7201 cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg aagaaatata tttgcatgtc
     7261 tttagttcta tgatgacaca aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg
     7321 cagatgcagt cggggcggcg cggtcccagg tccacttcgc atattaaggt gacgcgtgtg
     7381 gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag
     7441 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     7501 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     7561 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     7621 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     7681 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     7741 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     7801 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     7861 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     7921 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     7981 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     8041 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     8101 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     8161 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     8221 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     8281 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     8341 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     8401 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccgggagatg ggggaggcta
     8461 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     8521 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     8581 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     8641 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     8701 gcggcaagcc ctgccatagc cacgggcccc gtgggttagg gacggcggat cgcggccctc
     8761 tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca tttatattgg
//
