LOCUS       sequence-251969-       16156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Dysferlin protein (Therapeutic gene) along with Follistatin 
            protein to cure LGMD2B along with luciferase enzyme for in vivo live
            imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16156)
  AUTHORS   Guha TK, Pichavant C, Calos MP
  TITLE     Plasmid-Mediated Gene Therapy in Mouse Models of Limb Girdle 
            Muscular Dystrophy.
  JOURNAL   Mol Ther Methods Clin Dev. 2019 Oct 14;15:294-304. doi: 
            10.1016/j.omtm.2019.10.002. eCollection 2019 Dec 13.
  PUBMED    31890729
REFERENCE   2  (bases 1 to 16156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2019-10-14"; volume: "15:294-304. doi"; 
            pages: " 10.1016/j.omtm.2019.10.002. eCollection 2019 Dec 13"
COMMENT     Sequence Label: pKLD-CAG-DYSF-CFL
FEATURES             Location/Qualifiers
     source          1..16156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1406..2200
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1406..2200
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1460..1479)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2070..2089
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      2525..3113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3014..3033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    3177..3210
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3238..3307
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     enhancer        3563..3866
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3869..4144
                     /label=chicken beta-actin promoter
     intron          4145..5153
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5077..5099
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             5914..5925
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             11482..11538
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             11539..12246
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(11684..11702)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     11952..11971
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             12256..12312
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             13360..13416
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             13417..15069
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(13483..13501)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(15146..15165)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    15211..15266
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(15265..15284)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(15609..15628)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    15653..15700
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    15871..15920
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     primer_bind     complement(15926..15943)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    15932..16156
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gaattcaaac tagagggaca gccccccccc aaagccccca gggatgtaat tacgtccctc
       61 ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc gctccccccg
      121 catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg
      181 ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg
      241 aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg gcctgggttg
      301 caaaggagca cagtgctcat ccagatccaa ccccctgcta tgtgcagggt catcaaccag
      361 cagcccaggc tgcccagagc cacatccagc ctggccttga atgcctgcag ggatggggca
      421 tccacagcct ccttgggcaa cctgttcagt gcgtcaccac cctctggggg aaaaactgcc
      481 tcctcatatc caacccaaac ctcccctgtc tcagtgtaaa gccattcccc cttgtcctat
      541 caagggggag tttgctgtga cattgttggt ctggggtgac acatgtttgc caattcagtg
      601 catcacggag aggcagatct tggggataag gaagtgcagg acagcatgga cgtgggacat
      661 gcaggtgttg agggctctgg gacactctcc aagtcacagc gttcagaaca gccttaaaga
      721 taagaagata ggatagaagg acaaagagca agttaaaacc cagcatggag aggagcacaa
      781 aaaggccaca gacactgctg gtccctgtgt ctgagcctgc atgtttgatg gtgtctggat
      841 gcaagcagaa ggggtggaag agcttgcctg gagagataca gctgggtcag taggactggg
      901 acaggcagct ggagaattgc catgtagatg ttcatacaat cgtcaaatca tgaaggctgg
      961 aaaagccctc caagatcccc aagaccaacc ccaacccacc caccgtgccc actggccatg
     1021 tccctcagtg ccacatcccc acagttcttc atcacctcca gggacggtga cccccccacc
     1081 tccgtgggca gctgtgccac tgcagcaccg ctctttggag aaggtaaatc ttgctaaatc
     1141 cagcccgacc ctcccctggc acaacgtaag gccattatct ctcatccaac tccaggacgg
     1201 agtcagtgag gatggggctc tagtttgccg cctcttctac tgggcggttt tatggacagc
     1261 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt
     1321 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca
     1381 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     1441 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     1501 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     1561 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     1621 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     1681 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     1741 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     1801 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     1861 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     1921 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     1981 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     2041 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     2101 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     2161 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attattaacg cttacaattt
     2221 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tcaggtggca
     2281 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2341 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatagcac gtgctaaaac
     2401 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2461 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2521 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2581 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2641 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2701 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2761 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2821 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2881 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2941 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3001 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3061 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3121 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttcataa
     3181 cttcgtataa tgtatgctat acgaagttat cttaaggtcg acgatgtagg tcacggtctc
     3241 gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc
     3301 acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc
     3361 gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca cggtgagcac
     3421 gggacgtgcg acggcgtcgg cgggtgcgga tacgcggggc agcgtcagcg ggttctcgac
     3481 ggtcacggcg ggcatgtcga cctcgagatt aatagtaatc aattacgggg tcattagttc
     3541 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     3601 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     3661 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     3721 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     3781 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     3841 acgtattagt catcgctatt accatgggtc gaggtgagcc ccacgttctg cttcactctc
     3901 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     3961 gcagcgatgg gggcgggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg
     4021 gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     4081 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     4141 ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc
     4201 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     4261 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
     4321 aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt
     4381 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg
     4441 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg
     4501 ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     4561 gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac
     4621 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt
     4681 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     4741 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg
     4801 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     4861 agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc
     4921 ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     4981 cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg
     5041 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     5101 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     5161 gcaacgcgct ggttattgtg ctgtctcatc attttggcaa agaattcggt accgtttaaa
     5221 cgatcggccg ccaccatgct gagggtcttc atcctctatg ccgagaacgt ccacacaccc
     5281 gacaccgaca tcagcgatgc ctactgctcc gcggtgtttg caggggtgaa gaagagaacc
     5341 aaagtcatca agaacagcgt gaaccctgta tggaatgagg gatttgaatg ggacctcaag
     5401 ggcatccccc tggaccaggg ctctgagctt catgtggtgg tcaaagacca tgagacgatg
     5461 gggaggaaca ggttcctggg ggaagccaag gtcccactcc gagaggtcct cgccacccct
     5521 agtctgtccg ccagcttcaa tgcccccctg ctggacacca agaagcagcc cacaggggcc
     5581 tcgctggtcc tgcaggtgtc ctacacaccg ctgcctggag ctgtgcccct gttcccgccc
     5641 cctactcctc tggagccctc cccgactctg cctgacctgg atgtagtggc agacacagga
     5701 ggagaggaag acacagagga ccagggactc actggagatg aggcggagcc attcctggat
     5761 caaagcggag gcccgggggc tcccaccacc ccaaggaaac taccttcacg tcctccgccc
     5821 cactaccccg ggatcaaaag aaagcgaagt gcgcctacat ctagaaagct gctgtcagac
     5881 aaaccgcagg atttccagat cagggtccag gtgatcgagg ggcgccagct gccgggggtg
     5941 aacatcaagc ctgtggtcaa ggttaccgct gcagggcaga ccaagcggac gcggatccac
     6001 aagggaaaca gcccactctt caatgagact cttttcttca acttgtttga ctctcctggg
     6061 gagctgtttg atgagcccat ctttatcacg gtggtagact ctcgttctct caggacagat
     6121 gctctcctcg gggagttccg gatggacgtg ggcaccattt acagagagcc ccggcacgcc
     6181 tatctcagga agtggctgct gctctcagac cctgatgact tctctgctgg ggccagaggc
     6241 tacctgaaaa caagcctttg tgtgctgggg cctggggacg aagcgcctct ggagagaaaa
     6301 gacccctctg aagacaagga ggacattgaa agcaacctgc tccggcccac aggcgtagcc
     6361 ctgcgaggag cccacttctg cctgaaggtc ttccgggccg aggacttgcc gcagatggac
     6421 gatgccgtga tggacaacgt gaaacagatc tttggcttcg agagtaacaa gaagaacttg
     6481 gtggacccct ttgtggaggt cagctttgcg gggaaaatgc tgtgcagcaa gatcttggag
     6541 aagacggcca accctcagtg gaaccagaac atcacactgc ctgccatgtt tccctccatg
     6601 tgcgaaaaaa tgaggattcg tatcatagac tgggaccgcc tgactcacaa tgacatcgtg
     6661 gctaccacct acctgagtat gtcgaaaatc tctgcccctg gaggagaaat agaagaggag
     6721 cctgcaggtg ctgtcaagcc ttcgaaagcc tcagacttgg atgactacct gggcttcctc
     6781 cccacttttg ggccctgcta catcaacctc tatggcagtc ccagagagtt cacaggcttc
     6841 ccagacccct acacagagct caacacaggc aagggggaag gtgtggctta tcgtggccgg
     6901 cttctgctct ccctggagac caagctggtg gagcacagtg aacagaaggt ggaggacctt
     6961 cctgcggatg acatcctccg ggtggagaag taccttagga ggcgcaagta ctccctgttt
     7021 gcggccttct actcagccac catgctgcag gatgtggatg atgccatcca gtttgaggtc
     7081 agcatcggga actacgggaa caagttcgac atgacctgcc tgccgctggc ctccaccact
     7141 cagtacagcc gtgcagtctt tgacgggtgc cactactact acctaccctg gggtaacgtg
     7201 aaacctgtgg tggtgctgtc atcctactgg gaggacatca gccatagaat cgagactcag
     7261 aaccagctgc ttgggattgc tgaccggctg gaagctggcc tggagcaggt ccacctggcc
     7321 ctgaaggcgc agtgctccac ggaggacgtg gactcgctgg tggctcagct gacggatgag
     7381 ctcatcgcag gctgcagcca gcctctgggt gacatccatg agacaccctc tgccacccac
     7441 ctggaccagt acctgtacca gctgcgcacc catcacctga gccaaatcac tgaggctgcc
     7501 ctggccctga agctcggcca cagtgagctc cctgcagctc tggagcaggc ggaggactgg
     7561 ctcctgcgtc tgcgtgccct ggcagaggag ccccagaaca gcctgccgga catcgtcatc
     7621 tggatgctgc agggagacaa gcgtgtggca taccagcggg tgcccgccca ccaagtcctc
     7681 ttctcccggc ggggtgccaa ctactgtggc aagaattgtg ggaagctaca gacaatcttt
     7741 ctgaaatatc cgatggagaa ggtgcctggc gcccggatgc cagtgcagat acgggtcaag
     7801 ctgtggtttg ggctctctgt ggatgagaag gagttcaacc agtttgctga ggggaagctg
     7861 tctgtctttg ctgaaaccta tgagaacgag actaagttgg cccttgttgg gaactggggc
     7921 acaacgggcc tcacctaccc caagttttct gacgtcacgg gcaagatcaa gctacccaag
     7981 gacagcttcc gcccctcggc cggctggacc tgggctggag attggttcgt gtgtccggag
     8041 aagactctgc tccatgacat ggacgccggt cacctgagct tcgtggaaga ggtgtttgag
     8101 aaccagaccc ggcttcccgg aggccagtgg atctacatga gtgacaacta caccgatgtg
     8161 aacggggaga aggtgcttcc caaggatgac attgagtgcc cactgggctg gaagtgggaa
     8221 gatgaggaat ggtccacaga cctcaaccgg gctgtcgatg agcaaggctg ggagtatagc
     8281 atcaccatcc ccccggagcg gaagccgaag cactgggtcc ctgctgagaa gatgtactac
     8341 acacaccgac ggcggcgctg ggtgcgcctg cgcaggaggg atctcagcca aatggaagca
     8401 ctgaaaaggc acaggcaggc ggaggcggag ggcgagggct gggagtacgc ctctcttttt
     8461 ggctggaagt tccacctcga gtaccgcaag acagatgcct tccgccgccg ccgctggcgc
     8521 cgtcgcatgg agccactgga gaagacgggg cctgcagctg tgtttgccct tgagggggcc
     8581 ctgggcggcg tgatggatga caagagtgaa gattccatgt ccgtctccac cttgagcttc
     8641 ggtgtgaaca gacccacgat ttcctgcata ttcgactatg ggaaccgcta ccatctacgc
     8701 tgctacatgt accaggcccg ggacctggct gcgatggaca aggactcttt ttctgatccc
     8761 tatgccatcg tctccttcct gcaccagagc cagaagacgg tggtggtgaa gaacaccctt
     8821 aaccccacct gggaccagac gctcatcttc tacgagatcg agatctttgg cgagccggcc
     8881 acagttgctg agcaaccgcc cagcattgtg gtggagctgt acgaccatga cacttatggt
     8941 gcagacgagt ttatgggtcg ctgcatctgt caaccgagtc tggaacggat gccacggctg
     9001 gcctggttcc cactgacgag gggcagccag ccgtcggggg agctgctggc ctcttttgag
     9061 ctcatccaga gagagaagcc ggccatccac catattcctg gttttgaggt gcaggagaca
     9121 tcaaggatcc tggatgagtc tgaggacaca gacctgccct acccaccacc ccagagggag
     9181 gccaacatct acatggttcc tcagaacatc aagccagcgc tccagcgtac cgccatcgag
     9241 atcctggcat ggggcctgcg gaacatgaag agttaccagc tggccaacat ctcctccccc
     9301 agcctcgtgg tagagtgtgg gggccagacg gtgcagtcct gtgtcatcag gaacctccgg
     9361 aagaacccca actttgacat ctgcaccctc ttcatggaag tgatgctgcc cagggaggag
     9421 ctctactgcc cccccatcac cgtcaaggtc atcgataacc gccagtttgg ccgccggcct
     9481 gtggtgggcc agtgtaccat ccgctccctg gagagcttcc tgtgtgaccc ctactcggcg
     9541 gagagtccat ccccacaggg tggcccagac gatgtgagcc tactcagtcc tggggaagac
     9601 gtgctcatcg acattgatga caaggagccc ctcatcccca tccaggagga agagttcatc
     9661 gattggtgga gcaaattctt tgcctccata ggggagaggg aaaagtgcgg ctcctacctg
     9721 gagaaggatt ttgacaccct gaaggtctat gacacacagc tggagaatgt ggaggccttt
     9781 gagggcctgt ctgacttttg taacaccttc aagctgtacc ggggcaagac gcaggaggag
     9841 acagaagatc catctgtgat tggtgaattt aagggcctct tcaaaattta tcccctccca
     9901 gaagacccag ccatccccat gcccccaaga cagttccacc agctggccgc ccagggaccc
     9961 caggagtgct tggtccgtat ctacattgtc cgagcatttg gcctgcagcc caaggacccc
    10021 aatggaaagt gtgatcctta catcaagatc tccataggga agaaatcagt gagtgaccag
    10081 gataactaca tcccctgcac gctggagccc gtatttggaa agatgttcga gctgacctgc
    10141 actctgcctc tggagaagga cctaaagatc actctctatg actatgacct cctctccaag
    10201 gacgaaaaga tcggtgagac ggtcgtcgac ctggagaaca ggctgctgtc caagtttggg
    10261 gctcgctgtg gactcccaca gacctactgt gtctctggac cgaaccagtg gcgggaccag
    10321 ctccgcccct cccagctcct ccacctcttc tgccagcagc atagagtcaa ggcacctgtg
    10381 taccggacag accgtgtaat gtttcaggat aaagaatatt ccattgaaga gatagaggct
    10441 ggcaggatcc caaacccaca cctgggccca gtggaggagc gtctggctct gcatgtgctt
    10501 cagcagcagg gcctggtccc ggagcacgtg gagtcacggc ccctctacag ccccctgcag
    10561 ccagacatcg agcaggggaa gctgcagatg tgggtcgacc tatttccgaa ggccctgggg
    10621 cggcctggac ctcccttcaa catcacccca cggagagcca gaaggttttt cctgcgttgt
    10681 attatctgga ataccagaga tgtgatcctg gatgacctga gcctcacggg ggagaagatg
    10741 agcgacattt atgtgaaagg ttggatgatt ggctttgaag aacacaagca aaagacagac
    10801 gtgcattatc gttccctggg aggtgaaggc aacttcaact ggaggttcat tttccccttc
    10861 gactacctgc cagctgagca agtctgtacc attgccaaga aggatgcctt ctggaggctg
    10921 gacaagactg agagcaaaat cccagcacga gtggtgttcc agatctggga caatgacaag
    10981 ttctcctttg atgattttct gggctccctg cagctcgatc tcaaccgcat gcccaagcca
    11041 gccaagacag ccaagaagtg ctccttggac cagctggatg atgctttcca cccagaatgg
    11101 tttgtgtccc tttttgagca gaaaacagtg aagggctggt ggccctgtgt agcagaagag
    11161 ggtgagaaga aaatactggc gggcaagctg gaaatgacct tggagattgt agcagagagt
    11221 gagcatgagg agcggcctgc tggccagggc cgggatgagc ccaacatgaa ccctaagctt
    11281 gaggacccaa ggcgccccga cacctccttc ctgtggttta cctccccata caagaccatg
    11341 aagttcatcc tgtggcggcg tttccggtgg gccatcatcc tcttcatcat cctcttcatc
    11401 ctgctgctgt tcctggccat cttcatctac gccttcccga actatgctgc catgaagctg
    11461 gtgaagccct tcagcacgcg tgccacgaac ttctctctgt taaagcaagc aggagacgtg
    11521 gaagaaaacc ccggtcctat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
    11581 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
    11641 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
    11701 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
    11761 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
    11821 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
    11881 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
    11941 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
    12001 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
    12061 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
    12121 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
    12181 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
    12241 tacaagaccg gtggtgccac gaacttctct ctgttaaagc aagcaggaga cgtggaagaa
    12301 aaccccggtc ctatggtccg cgcgaggcac cagccgggtg ggctttgcct cctgctgctg
    12361 ctgctctgcc agttcatgga ggaccgcagt gcccaggctg ggaactgctg gctccgtcaa
    12421 gcgaagaacg gccgctgcca ggtcctgtac aagaccgaac tgagcaagga ggagtgctgc
    12481 agcaccggcc ggctgagcac ctcgtggacc gaggaggacg tgaatgacaa cacactcttc
    12541 aagtggatga ttttcaacgg gggcgccccc aactgcatcc cctgtaaaga aacgtgtgag
    12601 aacgtggact gtggacctgg gaaaaaatgc cgaatgaaca agaagaacaa accccgctgc
    12661 gtctgcgccc cggattgttc caacatcacc tggaagggtc cagtctgcgg gctggatggg
    12721 aaaacctacc gcaatgaatg tgcactccta aaggcaagat gtaaagagca gccagaactg
    12781 gaagtccagt accaaggcag atgtaaaaag acttgtcggg atgttttctg tccaggcagc
    12841 tccacatgtg tggtggacca gaccaataat gcctactgtg tgacctgtaa tcggatttgc
    12901 ccagagcctg cttcctctga gcaatatctc tgtgggaatg atggagtcac ctactccagt
    12961 gcctgccacc tgagaaaggc tacctgcctg ctgggcagat ctattggatt agcctatgag
    13021 ggaaagtgta tcaaagcaaa gtcctgtgaa gatatccagt gcactggtgg gaaaaaatgt
    13081 ttatgggatt tcaaggttgg gagaggccgg tgttccctct gtgatgagct gtgccctgac
    13141 agtaagtcgg atgagcctgt ctgtgccagt gacaatgcca cttatgccag cgagtgtgcc
    13201 atgaaggaag ctgcctgctc ctcaggtgtg ctactggaag taaagcactc cggatcttgc
    13261 aactccattt cggaagacac cgaggaagag gaggaagatg aagaccagga ctacagcttt
    13321 cctatatctt ctattctaga gtggaccggt ggatccggtg ccacgaactt ctctctgtta
    13381 aagcaagcag gagacgtgga agaaaacccc ggtcctatgg aagacgccaa aaacataaag
    13441 aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag
    13501 gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag
    13561 gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa
    13621 cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc
    13681 tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt
    13741 tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt
    13801 tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa
    13861 attattatca tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc
    13921 acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg
    13981 gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc
    14041 gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc
    14101 aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga
    14161 atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt
    14221 gaagaagagc tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg
    14281 ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat
    14341 ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc
    14401 aagaggttcc atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct
    14461 attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt
    14521 tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc
    14581 gaactgtgtg tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc
    14641 aacgccttga ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa
    14701 gacgaacact tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag
    14761 gtggctcccg ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt
    14821 gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag
    14881 cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc
    14941 gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga
    15001 aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc
    15061 gccgtgtaag cggccgcgtg caggctgcct atcagaaggt ggtggctggt gtggccaatg
    15121 ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca
    15181 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
    15241 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
    15301 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
    15361 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
    15421 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
    15481 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
    15541 tcctctcctg actactccca gtcatagctg tccctcttct ctctagagga tcctaggggg
    15601 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
    15661 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
    15721 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
    15781 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
    15841 gtcggggcgg caggccctgc catagcctcg tcggccggct tgtcgacgac ggcggtctcc
    15901 gtcgtcagga tcatccgggc cacaccctcg actgtgcctt ctagttgcca gccatctgtt
    15961 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
    16021 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
    16081 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
    16141 gcggtgggct ctatgg
//
