LOCUS       sequence-251976-        4121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  asymmetric plasmid partitioning E. coli - regulatory plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pSM034
FEATURES             Location/Qualifiers
     source          1..4121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             154..1125
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     terminator      1270..1297
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(1428..2219,2220..2288))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2051..2070
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2289..2393)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2814..2831)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2948..3493)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3028..3047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3569..3588
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    3746..3762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3751..3773
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             3964..3993
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 catagcattt ttatccataa gattagcgga tcctaccttt tacttcttgc catcgactac
       61 ggatactgag cacatgcatg ccacttaaaa aggagatcaa caatgaaagc aattttcgta
      121 ctgaaacatc ttaatcatgc taaggagact agtatgaagc gtgcgcctgt tattccaaaa
      181 catacgctca atactcaacc ggttgaagat acttcgttat cgacaccagc tgccccgatg
      241 gtggattcgt taattgcgcg cgtaggagta atggctcgcg gtaatgccat tactttgcct
      301 gtatgtggtc gggatgtgaa gtttactctt gaagtgctcc ggggtgatag tgttgagaag
      361 acctctcggg tatggtcagg taatgaacgt gaccaggagc tgcttactga ggacgcactg
      421 gatgatctca tcccttcttt tctactgact ggtcaacaga caccggcgtt cggtcgaaga
      481 gtatctggtg tcatagaaat tgccgatggg agtcgccgtc gtaaagctgc tgcacttacc
      541 gaaagtgatt atcgtgttct ggttggcgag ctggatgatg agcagatggc tgcattatcc
      601 agattgggta acgattatcg cccaacaagt gcttatgaac gtggtcagcg ttatgcaagc
      661 cgattgcaga atgaatttgc tggaaatatt tctgcgctgg ctgatgcgga aaatatttca
      721 cgtaagatta ttacccgctg tatcaacacc gccaaattgc ctaaatcagt tgttgctctt
      781 ttttctcacc ccggtgaact atctgcccgg tcaggtgatg cacttcaaaa agcctttaca
      841 gataaagagg aattacttaa gcagcaggca tctaaccttc atgagcagaa aaaagctggg
      901 gtgatatttg aagctgaaga agttatcact cttttaactt ctgtgcttaa aacgtcatct
      961 gcatcaagaa ctagtttaag ctcacgacat cagtttgctc ctggagcgac agtattgtat
     1021 aagggcgata aaatggtgct taacctggac aggtctcgtg ttccaactga gtgtatagag
     1081 aaaattgagg ccattcttaa ggaacttgaa aagccagcac cctgaaagct tgctacgtgc
     1141 tgctgaagtt gcccgcaaca gagagtggaa ccaaccggtg ataccacgat actatgactg
     1201 agagtcaacg ccatgagcgg cctcatttct tattctgagt tacaacagtc cgcaccgctg
     1261 tctcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat
     1321 aaaaagccag attattaatc cggctttttt attatttcct agctagccgc acttattcag
     1381 gcgtagcaac caggcgttta agggcaccaa taactgcctt aaaaaaatta ccaatgctta
     1441 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1501 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1561 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1621 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1681 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1741 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     1801 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     1861 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1921 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1981 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2041 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2101 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2161 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2221 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2281 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2341 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2401 ccccgaaaag tgccacctga cgtcgtctca ttttcgccaa aagttggccc agggcttccc
     2461 ggtatcaaca gggacaccag aatttattta ttctgcgaag tgatcttccg tcacaggtat
     2521 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
     2581 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
     2641 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
     2701 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
     2761 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
     2821 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
     2881 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
     2941 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
     3001 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
     3061 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     3121 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     3181 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     3241 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     3301 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     3361 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     3421 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     3481 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctagg ctcatgagcc
     3541 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     3601 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt
     3661 tgacagctta tcgagctcag tgcgctcgag cttcccaacc ttaccagagg gcgccccagc
     3721 tggcaattcc gacgtctgtg tggaattgtg agcggataac aatttcacac agggccctcg
     3781 gacaccgagg agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca gaggaggaac
     3841 gagctaaaac ggagcttttt tgccctgcgt gaccagatcc cggagttgga aaacaatgaa
     3901 aaggccccca aggtagttat ccttaaaaaa gccacagcat acatcctgtc cgtccaagca
     3961 gaggagcaaa agctcatttc tgaagaggac ttgttgcgga aacgacgaga acagttgaaa
     4021 cacaaacttg aacagctacg gaactcttgt gcgtaaggaa aagtaaggaa aacgattcct
     4081 tctaacagaa atgtcctgag caatcaccta tgaactgtcg a
//
