LOCUS       sequence-251977-        3418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  asymmetric plasmid partitioning E. coli - regulatory plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Molinari S, Shis DL, Bhakta SP, Chappell J, Igoshin OA, Bennett MR
  TITLE     A synthetic system for asymmetric cell division in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):917-924. doi: 
            10.1038/s41589-019-0339-x. Epub 2019 Aug 12.
  PUBMED    31406375
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0339-x"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "917-924"
COMMENT     Sequence Label: pSPB847
FEATURES             Location/Qualifiers
     source          1..3418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             153..161
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    1132..1152
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(join(1380..2171,2172..2240))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2003..2022
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2241..2345)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(2389..2420)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     complement(2564..2581)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2698..3243)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2778..2797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3310..3321
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    3368..3389
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 cgcacccata gcatttttat ccataagatt agcggatcct accttttact tcttgccatc
       61 gactacggat actgagcacg acgggcccaa gttcacttaa aaaggagatc aacaatgaaa
      121 gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg tccgaagggc
      181 gtcgtggtct gggtcgaggg ctttcggccc tgctgggcga ggtcgacgcc gcgccggctc
      241 aggcgccggg cgagcagctt ggcggttccc gtgaagcgcc gatcgaaatc ctgcagcgca
      301 atcccgacca gccccgtcgg accttccgcg aggaagacct cgaggatctg tcaaactcga
      361 tccgtgagaa gggcgttctg cagccgatcc tggtgcggcc gtcgccggat acggctggcg
      421 agtaccagat cgtcgccggc gagcgccgtt ggcgcgcagc ccagcgggcg ggcctcaaga
      481 ctgtgccgat catggtgcgc gagctcgacg acctggcggt gctggagatc ggcatcatcg
      541 agaacgtcca gcgggctgat ctgaacgtcc tggaagaagc gctctcctac aaggtgctga
      601 tggagaagtt cgagcgcacc caggagaaca tcgcccagac catcggcaag agccgcagcc
      661 acgtggccaa cacgatgcgc ttgctggccc taccggacga ggttcagtcg cacctggtca
      721 gtggtgagct gacggccggc cacgcccgcg ccatcgctgc agcggctgac ccggtggccc
      781 tcgccaagca aatcatcgag ggcggcttgt cggtgcgcga gacagaggcc ctggcccgta
      841 aggcgcccaa cctgtctgcg ggcaagagca aggggggccg ccccccacgc gtgaaggaca
      901 ccgacaccca ggcgctggag tctgacctgt cctcggttct gggtctggat gtctcgatcg
      961 accatcgcgg gtctaccggc acgctgacca tcacctacgc caccctcgag cagctcgacg
     1021 acctctgcaa ccgactgacg cgcgggatct aataatggcc tcggtaccaa agacgaacaa
     1081 taagacgctg aaaagcgtct tttttcgttt tggtccgctg cgtttgagac gtactagtag
     1141 cggccgctgc agttaatcac tgattaactc ggtaccaaat tccagaaaag aggcctcccg
     1201 aaaggggggc cttttttcgt tttggtcctt ttgttatcaa taaaaaaggg gagcggtttc
     1261 ccgctcccct tattgttcgt ctacagatta tcaaaaagga tcttcaccta gatcctttta
     1321 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1381 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1441 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1501 agtgctgcaa tgataccgcg ggacccacgc tcaccggctc cagatttatc agcaataaac
     1561 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     1621 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     1681 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     1741 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     1801 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     1861 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     1921 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     1981 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2041 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2101 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     2161 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2221 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2281 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2341 ccgcgcacat ttccccgaaa agtgccacct ggagtcctgt tgagtaatag tcaaaagcct
     2401 ccggtcggag gcttttgact ttctgcttac ccaatagcgg agtgtatact ggcttactat
     2461 gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac
     2521 cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc
     2581 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc
     2641 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt
     2701 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
     2761 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc
     2821 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
     2881 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
     2941 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3001 cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta
     3061 gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct
     3121 cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg
     3181 ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct
     3241 caagaagatc atcttattaa acggaaaaaa aaaccccgcc cctgacaggg cggggttttt
     3301 tttccaatta ttgaaggccg ctaacgcggc ctttttttgt ttctggtctg ccttaatcaa
     3361 tgactaagaa ttcgcggccg cttctagagc gtctcacttc ggatccgctg caggcttc
//
