LOCUS       sequence-252012-       12656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid for expressing human codon-optimized AsCpf1 
            containing 3x N-terminal MYC-NLS signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12656)
  AUTHORS   Liu J, Srinivasan S, Li CY, Ho IL, Rose J, Shaheen M, Wang G, Yao W,
            Deem A, Bristow C, Hart T, Draetta G
  TITLE     Pooled library screening with multiplexed Cpf1 library.
  JOURNAL   Nat Commun. 2019 Jul 17;10(1):3144. doi: 10.1038/s41467-019-10963-x.
  PUBMED    31316073
REFERENCE   2  (bases 1 to 12656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-07-17"; volume: "10(1):3144. doi"; pages: " 
            10.1038/s41467-019-10963-x"
COMMENT     Sequence Label: LentiAsCpf1-3xMYC-blast
FEATURES             Location/Qualifiers
     source          1..12656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2413..2624
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2632..2641
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2644..2670
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             2671..2697
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             2698..2724
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             2725..6642
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      6362..6371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6652..6708
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6709..7107
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7132..7720
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7185..7205)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7603..7614)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7723..7739)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7724..7740)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8245..8425
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8451..8468)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8457..8681
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8727..9155
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8814..8833)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9024..9045
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9164..9184)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9329..9463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9390..9409
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9525..9572
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9591..9965
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10095..10216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10132..10151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10186..10205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10265..10281)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10265..10281)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10278..10300)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10289..10305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10313..10319,10320..10337,10338..10343))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10358..10379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10496..10513)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10667..11255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10747..10766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11426..12217,12218..12286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12049..12068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12287..12391)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12466..12485)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttagctagct aggtcttgaa aggagtggga attggctccg
     2401 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     2461 tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     2521 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     2581 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggaccgg tgccaccatg
     2641 gcgccagcgg caaagcgcgt aaaattggac ccagcagcca aacgagtaaa actcgatccg
     2701 gctgctaaac gagttaagct ggacacacag ttcgagggct ttaccaacct gtatcaggtg
     2761 agcaagacac tgcggtttga gctgatccca cagggcaaga ccctgaagca catccaggag
     2821 cagggcttca tcgaggagga caaggcccgc aatgatcact acaaggagct gaagcccatc
     2881 atcgatcgga tctacaagac ctatgccgac cagtgcctgc agctggtgca gctggattgg
     2941 gagaacctga gcgccgccat cgactcctat agaaaggaga aaaccgagga gacaaggaac
     3001 gccctgatcg aggagcaggc cacatatcgc aatgccatcc acgactactt catcggccgg
     3061 acagacaacc tgaccgatgc catcaataag agacacgccg agatctacaa gggcctgttc
     3121 aaggccgagc tgtttaatgg caaggtgctg aagcagctgg gcaccgtgac cacaaccgag
     3181 cacgagaacg ccctgctgcg gagcttcgac aagtttacaa cctacttctc cggcttttat
     3241 gagaacagga agaacgtgtt cagcgccgag gatatcagca cagccatccc acaccgcatc
     3301 gtgcaggaca acttccccaa gtttaaggag aattgtcaca tcttcacacg cctgatcacc
     3361 gccgtgccca gcctgcggga gcactttgag aacgtgaaga aggccatcgg catcttcgtg
     3421 agcacctcca tcgaggaggt gttttccttc cctttttata accagctgct gacacagacc
     3481 cagatcgacc tgtataacca gctgctggga ggaatctctc gggaggcagg caccgagaag
     3541 atcaagggcc tgaacgaggt gctgaatctg gccatccaga agaatgatga gacagcccac
     3601 atcatcgcct ccctgccaca cagattcatc cccctgttta agcagatcct gtccgatagg
     3661 aacaccctgt ctttcatcct ggaggagttt aagagcgacg aggaagtgat ccagtccttc
     3721 tgcaagtaca agacactgct gagaaacgag aacgtgctgg agacagccga ggccctgttt
     3781 aacgagctga acagcatcga cctgacacac atcttcatca gccacaagaa gctggagaca
     3841 atcagcagcg ccctgtgcga ccactgggat acactgagga atgccctgta tgagcggaga
     3901 atctccgagc tgacaggcaa gatcaccaag tctgccaagg agaaggtgca gcgcagcctg
     3961 aagcacgagg atatcaacct gcaggagatc atctctgccg caggcaagga gctgagcgag
     4021 gccttcaagc agaaaaccag cgagatcctg tcccacgcac acgccgccct ggatcagcca
     4081 ctgcctacaa ccctgaagaa gcaggaggag aaggagatcc tgaagtctca gctggacagc
     4141 ctgctgggcc tgtaccacct gctggactgg tttgccgtgg atgagtccaa cgaggtggac
     4201 cccgagttct ctgcccggct gaccggcatc aagctggaga tggagccttc tctgagcttc
     4261 tacaacaagg ccagaaatta tgccaccaag aagccctact ccgtggagaa gttcaagctg
     4321 aactttcaga tgcctacact ggcctctggc tgggacgtga ataaggagaa gaacaatggc
     4381 gccatcctgt ttgtgaagaa cggcctgtac tatctgggca tcatgccaaa gcagaagggc
     4441 aggtataagg ccctgagctt cgagcccaca gagaaaacca gcgagggctt tgataagatg
     4501 tactatgact acttccctga tgccgccaag atgatcccaa agtgcagcac ccagctgaag
     4561 gccgtgacag cccactttca gacccacaca acccccatcc tgctgtccaa caatttcatc
     4621 gagcctctgg agatcacaaa ggagatctac gacctgaaca atcctgagaa ggagccaaag
     4681 aagtttcaga cagcctacgc caagaaaacc ggcgaccaga agggctacag agaggccctg
     4741 tgcaagtgga tcgacttcac aagggatttt ctgtccaagt ataccaagac aacctctatc
     4801 gatctgtcta gcctgcggcc atcctctcag tataaggacc tgggcgagta ctatgccgag
     4861 ctgaatcccc tgctgtacca catcagcttc cagagaatcg ccgagaagga gatcatggat
     4921 gccgtggaga caggcaagct gtacctgttc cagatctata acaaggactt tgccaagggc
     4981 caccacggca agcctaatct gcacacactg tattggaccg gcctgttttc tccagagaac
     5041 ctggccaaga caagcatcaa gctgaatggc caggccgagc tgttctaccg ccctaagtcc
     5101 aggatgaaga ggatggcaca ccggctggga gagaagatgc tgaacaagaa gctgaaggat
     5161 cagaaaaccc caatccccga caccctgtac caggagctgt acgactatgt gaatcacaga
     5221 ctgtcccacg acctgtctga tgaggccagg gccctgctgc ccaacgtgat caccaaggag
     5281 gtgtctcacg agatcatcaa ggataggcgc tttaccagcg acaagttctt tttccacgtg
     5341 cctatcacac tgaactatca ggccgccaat tccccatcta agttcaacca gagggtgaat
     5401 gcctacctga aggagcaccc cgagacacct atcatcggca tcgatcgggg cgagagaaac
     5461 ctgatctata tcacagtgat cgactccacc ggcaagatcc tggagcagcg gagcctgaac
     5521 accatccagc agtttgatta ccagaagaag ctggacaaca gggagaagga gagggtggca
     5581 gcaaggcagg cctggtctgt ggtgggcaca atcaaggatc tgaagcaggg ctatctgagc
     5641 caggtcatcc acgagatcgt ggacctgatg atccactacc aggccgtggt ggtgctggag
     5701 aacctgaatt tcggctttaa gagcaagagg accggcatcg ccgagaaggc cgtgtaccag
     5761 cagttcgaga agatgctgat cgataagctg aattgcctgg tgctgaagga ctatccagca
     5821 gagaaagtgg gaggcgtgct gaacccatac cagctgacag accagttcac ctcctttgcc
     5881 aagatgggca cccagtctgg cttcctgttt tacgtgcctg ccccatatac atctaagatc
     5941 gatcccctga ccggcttcgt ggaccccttc gtgtggaaaa ccatcaagaa tcacgagagc
     6001 cgcaagcact tcctggaggg cttcgacttt ctgcactacg acgtgaaaac cggcgacttc
     6061 atcctgcact ttaagatgaa cagaaatctg tccttccaga ggggcctgcc cggctttatg
     6121 cctgcatggg atatcgtgtt cgagaagaac gagacacagt ttgacgccaa gggcacccct
     6181 ttcatcgccg gcaagagaat cgtgccagtg atcgagaatc acagattcac cggcagatac
     6241 cgggacctgt atcctgccaa cgagctgatc gccctgctgg aggagaaggg catcgtgttc
     6301 agggatggct ccaacatcct gccaaagctg ctggagaatg acgattctca cgccatcgac
     6361 accatggtgg ccctgatccg cagcgtgctg cagatgcgga actccaatgc cgccacaggc
     6421 gaggactata tcaacagccc cgtgcgcgat ctgaatggcg tgtgcttcga ctcccggttt
     6481 cagaacccag agtggcccat ggacgccgat gccaatggcg cctaccacat cgccctgaag
     6541 ggccagctgc tgctgaatca cctgaaggag agcaaggatc tgaagctgca gaacggcatc
     6601 tccaatcagg actggctggc ctacatccag gagctgcgca acggatccgg cgcaacaaac
     6661 ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgat ggccaagcct
     6721 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     6781 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     6841 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     6901 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     6961 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     7021 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     7081 ccctctggtt atgtgtggga gggctaagaa ttcgatatca agcttatcgg taatcaacct
     7141 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7201 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7261 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7321 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7381 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7441 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7501 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7561 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7621 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7681 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     7741 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     7801 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     7861 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     7921 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     7981 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     8041 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     8101 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     8161 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     8221 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     8281 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8341 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8401 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     8461 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     8521 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     8581 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     8641 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     8701 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     8761 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     8821 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8881 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8941 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     9001 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     9061 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     9121 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     9181 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9241 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9301 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccag ttccgcccat
     9361 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     9421 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9481 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     9541 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     9601 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     9661 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     9721 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     9781 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     9841 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     9901 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     9961 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
    10021 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
    10081 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    10141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    10201 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
    10261 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    10321 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    10381 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    10441 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    10501 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    10561 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    10621 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    10681 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    10741 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    10801 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    10861 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    10921 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10981 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    11041 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    11101 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    11161 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    11221 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    11281 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    11341 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    11401 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    11461 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    11521 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    11581 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    11641 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    11701 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    11761 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    11821 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    11881 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    11941 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    12001 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    12061 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    12121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    12181 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    12241 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    12301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    12361 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    12421 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
    12481 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
    12541 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
    12601 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
