LOCUS       sequence-25203-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.33
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..87
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    405..528
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(599..616)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(599..615)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(608..630)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      complement(683..726)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tttnnttttn actgatagtg ncctgttcgt tgcaacaaat tgataagcaa tgctttttta
       61 taatgccaac tttgtataat aaagttgtgt ttgttgtttt tgcgtaatga attattttag
      121 tgttgaaata attttattta atttttagag ttttcgtgtc caaatttatc tggattcatt
      181 aaattcgaaa aaaaaaaaca aaacaatgaa caataaacag aaagaaacaa taacacaact
      241 gcatgaaaag caataaaaca acaaaaaaga aaagaaaaaa caaaactggg caatgataag
      301 ggtggggatg gcacaacatc gatcttaaaa tcgaaacgaa accattgcaa cagtttaaaa
      361 tattacaaaa caacagaaca taagtgggtg ggatgagatt tatcccactt tgtacaagaa
      421 agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
      481 aaaacagact acataatact gtaaaacaca acatatgcag tcactatgaa ccaactactt
      541 agatggtatt agtgacctgt agaattcgag ctctagagct gcagggcccg agcttaagac
      601 tggccgtcgt tttacaacgt cgtgactggg aaaacatcca tgcnnnnnnt taacgcgaga
      661 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcggaagant gggcctttcg
      721 ttttatctgn tgtttgncgg tgaacnctct cctg
//
