LOCUS       sequence-25204-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.32
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..88
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    283..406
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(477..494)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(477..493)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(486..508)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      557..643
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tttnnntttt nactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
       61 ataatgccaa ctttgtataa taaagttggt gctgtgtcgg gcgattttat aggaggaatt
      121 ttgattcgat tatatttatg gttttcagaa atttgaagga aggaaaatgt attcagaaac
      181 atgagattct agggaaataa cagttaaaca agtagatctt gagatttgaa agtgatatca
      241 acgggaaaaa tttaagaaaa aaagtcaaga aaaaagatta tcccactttg tacaagaaag
      301 ttgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
      361 aacagactac ataatactgt aaaacacaac atatgcagtc actatgaacc aactacttag
      421 atggtattag tgacctgtag aattcgagct ctagagctgc agggcccgag cttaagactg
      481 gccgtcgttt tacaacgtcg tgactgggaa aacatccatg ctagcgttaa cgcgagagta
      541 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg gaagactggg cctttcgttt
      601 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
      661 gaacgttgtg aagcacnncc ggnnnngggn nggnngggca ggacgcccgc cataaactgc
      721 caggcatcna
//
