LOCUS       sequence-252040-        9117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Meyer C, Garzia A, Morozov P, Molina H, Tuschl T
  TITLE     The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 
            40S Subunits of Ribosomes Stalled in Translation from Lysosomal 
            Degradation.
  JOURNAL   Mol Cell. 2020 Mar 19;77(6):1193-1205.e5. doi: 
            10.1016/j.molcel.2019.12.024. Epub 2020 Jan 24.
  PUBMED    31981475
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.12.024"; journalName: "Mol Cell"; date: 
            "2020-03-19- 19"; volume: "77"; issue: "6"; pages: "1193-1205.e5"
COMMENT     Sequence Label: pLENTI_RPS2_K58R_Keima
FEATURES             Location/Qualifiers
     source          1..9117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    198..315
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        398..777
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        778..981
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1007..1026
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1007..1025
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1971..1973,1974..1976,1977..2639)
                     /codon_start=1
                     /product="monomeric Keima-Red fluorescent protein, also 
                     known as mKeima"
                     /label=mKeima-Red
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     CDS             2658..2687
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2706..2729
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2763..3336
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2786..3338
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2930..2947)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3157..3176
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3338..3937
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3338..3357)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3834..3854
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(3967..4000)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4056..4644
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4109..4129)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4527..4538)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4647..4663)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4648..4664)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5173..5353
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5415..6003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5495..5514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6080..6739)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6654..6673
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6740..6844)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6919..6938)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7109..7488
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7490..7688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7706..7886
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7933..8058
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8555..8788
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8973..9017
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
       61 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
      121 acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg
      181 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
      241 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
      301 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gttagtaccg
      361 ggcccgctct agacatgtcc aatatgaccg ccatgttgac attgattatt gactagttat
      421 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      481 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      541 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      601 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg
      661 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      721 ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      781 atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca
      841 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      901 ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg
      961 gaggtctata taagcagagc tcgtttagtg aaccgtcaga attttgtaat acgactcact
     1021 atagggcggc cgggaattcg tcgactggat ccggtaccga ggagatctgc cgccgcgatc
     1081 gccggcgcgc catggcggat gacgccggtg cagcgggggg gcccgggggc cctggtggcc
     1141 ctgggatggg gaaccgcggt ggcttccgcg gaggtttcgg cagtggcatc cggggccggg
     1201 gtcgcggccg tggacggggc cggggccgag gccgcggagc tcgcggaggc aaggccgagg
     1261 atagggagtg gatgcccgtc accaagttgg gccgcttggt caaggacatg aagatcaagt
     1321 ccctggagga gatctatctc ttctccctgc ccattaagga atcagagatc attgatttct
     1381 tcctgggggc ctctctcaag gatgaggttt tgaagattat gccagtgcag aagcagaccc
     1441 gtgccggcca gcgcaccagg ttcaaggcat ttgttgctat cggggactac aatggccacg
     1501 tcggtctggg tgttaagtgc tccaaggagg tggccaccgc catccgtggg gccatcatcc
     1561 tggccaagct ctccatcgtc cccgtgcgca gaggctactg ggggaacaag atcggcaagc
     1621 cccacactgt cccttgcaag gtgacaggcc gctgcggctc tgtgctggta cgcctcatcc
     1681 ctgcacccag gggcactggc atcgtctccg cacctgtgcc taagaagctg ctcatgatgg
     1741 ctggtatcga tgactgctac acctcagccc ggggctgcac tgccaccctg ggcaacttcg
     1801 ccaaggccac ctttgatgcc atttctaaga cctacagcta cctgaccccc gacctctgga
     1861 aggagactgt attcaccaag tctccctatc aggagttcac tgaccacctc gtcaagaccc
     1921 acaccagagt ctccgtgcag cggactcagg ctccagctgt ggctacaaca atggtgagtg
     1981 tgatcgctaa acaaatgacc tacaaggttt atatgtcagg cacggtcaat ggacactact
     2041 ttgaggtcga aggcgatgga aaaggaaagc cttacgaggg agagcagaca gtaaagctca
     2101 ctgtcaccaa gggtggacct ctgccatttg cttgggatat tttatcacca cagcttcagt
     2161 acggaagcat accattcacc aagtaccctg aagacatccc tgattatttc aagcagtcat
     2221 tccctgaggg atatacatgg gagaggagca tgaactttga agatggtgca gtgtgtactg
     2281 tcagcaatga ttccagcatc caaggcaact gtttcatcta caatgtcaaa atctctggtg
     2341 agaactttcc tcccaatgga cctgttatgc agaagaagac acagggctgg gaacccagca
     2401 ctgagcgtct ctttgcacga gatggaatgc tgataggaaa cgattatatg gctctgaagt
     2461 tggaaggagg tggtcactat ttgtgtgaat ttaaatctac ttacaaggca aagaagcctg
     2521 tgaggatgcc agggcgccac gagattgacc gcaaactgga tgtaaccagt cacaacaggg
     2581 attacacatc tgttgagcag tgtgaaatag ccattgcacg ccactctttg ctcggttaaa
     2641 cgcgtacgcg gccgctcgag cagaaactca tctcagaaga ggatctggca gcaaatgata
     2701 tcctggatta caaggatgac gacgataagg tttaaaccta ggcgtagcgg ccgcaaattc
     2761 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     2821 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     2881 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     2941 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     3001 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     3061 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     3121 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     3181 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     3241 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     3301 ggggacgtgg ttttcctttg aaaaacacga tgataatatg accgagtaca agcccacggt
     3361 gcgcctcgcc acccgcgacg acgtcccccg ggcagtacgc accctcgccg ccgcgttcgc
     3421 cgactacccc gccacgcgcc acaccgtcga tccagaccgc cacatcgagc gggtcaccga
     3481 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     3541 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     3601 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     3661 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     3721 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     3781 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     3841 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     3901 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgatgt acaagtagga ttcgtcgagg
     3961 gacctaataa cttcgtatag catacattat acgaagttat acatgtttaa gggttccggt
     4021 tccactaggt acaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     4081 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4141 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4201 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4261 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4321 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4381 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4441 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4501 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4561 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4621 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa
     4681 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     4741 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     4801 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     4861 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     4921 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     4981 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     5041 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     5101 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     5161 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5221 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5281 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5341 aaaatctcta gcagcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5401 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5461 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5521 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5581 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5641 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5701 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5761 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5821 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5881 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5941 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6001 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6061 taagggattt tggtcatgat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     6121 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     6181 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     6241 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     6301 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     6361 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     6421 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     6481 tatcccatat caccagctca ccgtctttca ttgccatacg gaactccgga tgagcattca
     6541 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     6601 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     6661 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     6721 cagtgatttt tttctccata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6781 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6841 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     6901 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     6961 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     7021 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     7081 tcgcgatgta cgggccagat atcgcgttga cattgattat tgactagtta ttaatagtaa
     7141 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     7201 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     7261 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     7321 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     7381 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     7441 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     7501 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     7561 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     7621 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     7681 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     7741 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7801 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7861 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     7921 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     7981 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     8041 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     8101 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     8161 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     8221 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     8281 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     8341 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     8401 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     8461 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     8521 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     8581 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     8641 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     8701 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     8761 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     8821 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     8881 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     8941 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     9001 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     9061 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtacttt
//
