LOCUS       sequence-252061-        2830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP 
            chromoprotein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: " 
            10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT     Sequence Label: amilGFP chromoprotein
FEATURES             Location/Qualifiers
     source          1..2830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..44)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        69..103
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             112..123
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    829..849
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      850..907
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(931..948)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1102..1690)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1182..1201)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1873..1967)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1988..2647)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2562..2581
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2809..2827
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
       61 ttctagagtt tacggctagc tcagtcctag gtacaatgct agctactaga gaaagaggag
      121 aaatactaga tgtcttattc aaagcatggc atcgtacaag aaatgaagac gaaataccat
      181 atggaaggca gtgtcaatgg ccatgaattt acgatcgaag gtgtaggaac tgggtaccct
      241 tacgaaggga aacagatgtc cgaattagtg atcatcaagc ctgcgggaaa accccttcca
      301 ttctcctttg acatactgtc atcagtcttt caatatggaa accgttgctt cacaaagtac
      361 ccggcagaca tgcctgacta tttcaagcaa gcattcccag atggaatgtc atatgaaagg
      421 tcatttctat ttgaggatgg agcagttgct acagccagct ggaacattcg tctcgaagga
      481 aattgcttca tccacaaatc catctttcat ggcgtaaact ttcccgctga tggacccgta
      541 atgaaaaaga agacaattga ctgggataag tccttcgaaa aaatgactgt gtctaaagag
      601 gtgctaagag gtgacgtgac tatgtttctt atgctcgaag gaggtggttc tcacagatgc
      661 caatttcact ccacttacaa aacagagaag ccggtcacac tgcccccgaa tcatgtcgta
      721 gaacatcaaa ttgtgaggac cgaccttggc caaagtgcaa aaggctttac agtcaagctg
      781 gaagcacatg ccgcggctca tgttaaccct ttgaaggtta aataataata ctagtagcgg
      841 ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc
      901 gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
      961 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1021 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1081 aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa
     1141 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1201 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1261 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1321 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1381 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1441 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1501 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1561 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1621 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1681 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1741 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1801 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1861 ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     1921 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     1981 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     2041 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     2101 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     2161 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     2221 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     2281 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     2341 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     2401 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     2461 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     2521 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     2581 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     2641 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     2701 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg
     2761 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2821 acgaggcaga
//
