LOCUS       sequence-252063-        2810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BioBrick pSB1C3 plasmid that constitutively overexpresses spisPink 
            chromoprotein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: " 
            10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT     Sequence Label: spisPink chromoprotein
FEATURES             Location/Qualifiers
     source          1..2810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..44)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        69..103
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     misc_feature    809..829
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      830..887
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(911..928)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1082..1670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1162..1181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1853..1947)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1968..2627)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2542..2561
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2789..2807
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
       61 ttctagagtt tacggctagc tcagtcctag gtacaatgct agctactaga gatttaatag
      121 gaggtagttg atgtcgcact caaaacaagc actggcagat acgatgaaaa tgacgtggct
      181 gatggaaggt tcggtcaacg gtcacgcctt tacgattgaa ggcgaaggta ccggcaaacc
      241 gtatgaaggt aaacagagtg gcacctttcg tgttacgaaa ggcggtccgc tgccgtttgc
      301 gtttgatatt gtcgctccga ccctgaaata cggtttcaaa tgcttcatga aatacccggc
      361 ggatattccg gactacttta aactggcctt cccggaaggc ctgacgtacg atcgtaaaat
      421 tgcgtttgaa gacggcggtt gtgcgaccgc cacggtcgaa atgagcctga agggtaacac
      481 cctggtgcat aaaacgaact ttcagggcgg taatttcccg attgatggtc cggtgatgca
      541 aaaacgcacc ctgggctggg aaccgacctc tgaaaaaatg acgccgtgcg atggtattat
      601 caaaggcgac accatcatgt acctgatggt tgaaggcggt aaaacgctga aatgtcgtta
      661 tgaaaacaat taccgcgcca acaaaccagt gctgatgccg ccgagccact ttgtggatct
      721 gcgtctgacc cgcacgaatc tggataaaga aggtctggcg ttcaaactgg aagaatatgc
      781 tgttgcccgt gtgctggaag tgtaataata ctagtagcgg ccgctgcagt ccggcaaaaa
      841 agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta
      901 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      961 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1021 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1081 ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1141 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1201 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1261 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1321 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1381 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1441 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1501 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1561 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1621 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1681 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1741 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1801 aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac
     1861 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     1921 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     1981 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     2041 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     2101 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     2161 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     2221 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     2281 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     2341 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     2401 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     2461 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     2521 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     2581 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     2641 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     2701 tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac
     2761 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
//
