LOCUS       sequence-252076-        3719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 vector with alternative selection marker (chloramphenicol). 
            OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 91 to 125)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP39-1A(sfGFP)
FEATURES             Location/Qualifiers
     source          1..3719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        91..125
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             130..150
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             167..877
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    930..950
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      984..1078
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     oriT            2096..2204
                     /note="incP origin of transfer"
     rep_origin      2240..2828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2729..2748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2982..2999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3014..3154
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3080..3099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3240..3262
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3256..3447)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3627..3713)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgccttgga acacctgctt ttcgctgaat tcgcggccgc
       61 ttctagagcg tctctggagt gagaccggag ttgacggcta gctcagtcct aggtacagtg
      121 ctagcgtact tgtttaactt taagaaggag atatacaatg gtagcccgta aaggcgaaga
      181 gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa
      241 gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt
      301 catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga cgctgactta
      361 tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc
      421 cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta
      481 caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa
      541 aggcattgac tttaaagaag atggcaatat cctgggccat aagctggaat acaattttaa
      601 cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa
      661 aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac
      721 tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt
      781 tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc
      841 agcgggcatc acgcatggta tggatgaact gtacaaaggt tcgtaacttc tgactgagtt
      901 gcacgctggt ctctcgctaa tgtgagacgt actagtagcg gccgctgcag ggagttgtct
      961 tcgaagactt cgctctagtc ttggactcct gttgatagat ccagtaatga cctcagaact
     1021 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc
     1081 aggggtcccc aataattacg atttaaataa caaacgggat tgacttttaa aaaaggattg
     1141 attctaatga agaaagcaga caagtaagcc tcctaaattc actttagata aaaatttagg
     1201 aggcatatca aatgaacttt aataaaattg atttagacaa ttggaagaga aaagagatat
     1261 ttaatcatta tttgaaccaa caaacgactt ttagtataac cacagaaatt gatattagtg
     1321 ttttataccg aaacataaaa caagaaggat ataaatttta ccctgcattt attttcttag
     1381 tgacaagggt gataaactca aatacagctt ttagaactgg ttacaatagc gacggagagt
     1441 taggttattg ggataagtta gagccacttt atacaatttt tgatggtgta tctaaaacat
     1501 tctctggtat ttggactcct gtaaagaatg acttcaaaga gttttatgat ttataccttt
     1561 ctgatgtaga gaaatataat ggttcgggga aattgtttcc caaaacacct atacctgaaa
     1621 atgctttttc tctttctatt attccatgga cttcatttac tgggtttaac ttaaatatca
     1681 ataataatag taattacctt ctacccatta ttacagcagg aaaattcatt aataaaggta
     1741 attcaatata tttaccgcta tctttacagg tacatcattc tgtttgtgat ggttatcatg
     1801 caggattgtt tatgaactct attcaggaat tgtcagatag gcctaatgac tggcttttat
     1861 aatatgagat aatgccgact gtacttttta cagtcggttt tctaatgtca ctagggctcg
     1921 cctttgggaa gtttgaaggg acttcggtcc gcgcgttgtc cttttccgct gcataaccct
     1981 gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca cgatatacag
     2041 gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt cagccgggca
     2101 ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc
     2161 acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccgtaggcc ggccccgtag
     2221 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2281 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2341 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     2401 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2461 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2521 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2581 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     2641 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2701 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2761 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2821 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2881 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     2941 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3001 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3061 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     3121 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     3181 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgacagtctc
     3241 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
     3301 gtaaagctca tcagcgtggt cgtgcagcga ttcacagatg tctgcctgtt catccgcgtc
     3361 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
     3421 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
     3481 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
     3541 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcgggg
     3601 gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa
     3661 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcct
//
