LOCUS       sequence-252086-        3890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low 
            copy number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 91 to 125)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP27ts-1A(sfGFP)
FEATURES             Location/Qualifiers
     source          1..3890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        91..125
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             130..150
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             167..877
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    930..950
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      984..1078
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1199..1218)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1210..2025
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2192..2300
                     /note="incP origin of transfer"
     CDS             complement(2311..3261)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFDIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3309..3531
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3798..3884)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgccttgga acacctgctt ttcgctgaat tcgcggccgc
       61 ttctagagcg tctctggagt gagaccggag ttgacggcta gctcagtcct aggtacagtg
      121 ctagcgtact tgtttaactt taagaaggag atatacaatg gtagcccgta aaggcgaaga
      181 gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa
      241 gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt
      301 catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga cgctgactta
      361 tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc
      421 cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta
      481 caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa
      541 aggcattgac tttaaagaag atggcaatat cctgggccat aagctggaat acaattttaa
      601 cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa
      661 aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac
      721 tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt
      781 tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc
      841 agcgggcatc acgcatggta tggatgaact gtacaaaggt tcgtaacttc tgactgagtt
      901 gcacgctggt ctctcgctaa tgtgagacgt actagtagcg gccgctgcag ggagttgtct
      961 tcgaagactt cgctctagtc ttggactcct gttgatagat ccagtaatga cctcagaact
     1021 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc
     1081 aggggtcccc aataattacg atttaaattt gtgtctcaaa atctctgatg ttacattgca
     1141 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1201 aggggtgtta tgagccatat tcagcgtgaa acgagctgta gccgtccgcg tctgaacagc
     1261 aacatggatg cggatctgta tggctataaa tgggcgcgtg ataacgtggg tcagagcggc
     1321 gcgaccattt atcgtctgta tggcaaaccg gatgcgccgg aactgtttct gaaacatggc
     1381 aaaggcagcg tggcgaacga tgtgaccgat gaaatggtgc gtctgaactg gctgaccgaa
     1441 tttatgccgc tgccgaccat taaacatttt attcgcaccc cggatgatgc gtggctgctg
     1501 accaccgcga ttccgggcaa aaccgcgttt caggtgctgg aagaatatcc ggatagcggc
     1561 gaaaacattg tggatgcgct ggccgtgttt ctgcgtcgtc tgcatagcat tccggtgtgc
     1621 aactgcccgt ttaacagcga tcgtgtgttt cgtctggccc aggcgcagag ccgtatgaac
     1681 aacggcctgg tggatgcgag cgattttgat gatgaacgta acggctggcc ggtggaacag
     1741 gtgtggaaag aaatgcataa actgctgccg tttagcccgg atagcgtggt gacccacggc
     1801 gattttagcc tggataacct gattttcgat gaaggcaaac tgattggctg cattgatgtg
     1861 ggccgtgtgg gcattgcgga tcgttatcag gatctggcca ttctgtggaa ctgcctgggc
     1921 gaatttagcc cgagcctgca aaaacgtctg tttcagaaat atggcattga taatccggat
     1981 atgaacaaac tgcaatttca tctgatgctg gatgaatttt tctaataatt aattggaccg
     2041 cggtccgcgc gttgtccttt tccgctgcat aaccctgctt cggggtcatt atagcgattt
     2101 tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag
     2161 actttccttg gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg
     2221 cgagcgggtg ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc
     2281 ctgctctgcg aggctggccg taggccggcc tcagatcctt ccgtatttag ccagtatgtt
     2341 ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatactt
     2401 actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa
     2461 agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag gaatctgatg taatggttgt
     2521 tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat
     2581 ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac
     2641 caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg
     2701 gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc
     2761 aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca
     2821 ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt
     2881 tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa
     2941 tttttcgctt gagaacttgg catagtttgt ccactggaaa atatcaaagc ctttaaccaa
     3001 aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc
     3061 ataagcattt tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac
     3121 cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa
     3181 aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt
     3241 gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca
     3301 attgagatgg gctagtcaat gataattaca tgtccttttc ctttgagttg tgggtatctg
     3361 taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc
     3421 cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa
     3481 gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta
     3541 gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag
     3601 accttaaaac cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat
     3661 tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg
     3721 ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc ggcgcgccca
     3781 gctgtctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     3841 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
//
