LOCUS       sequence-252092-        2787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For course in ""Synthetic Biology: A Lab Manual"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: " 
            10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT     Sequence Label: amilGFP chromoprotein promoter-less
FEATURES             Location/Qualifiers
     source          1..2787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..44)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             69..80
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    786..806
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      807..864
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(888..905)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1059..1647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1139..1158)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1830..1924)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1945..2604)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2519..2538
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2766..2784
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
       61 ttctagagaa agaggagaaa tactagatgt cttattcaaa gcatggcatc gtacaagaaa
      121 tgaagacgaa ataccatatg gaaggcagtg tcaatggcca tgaatttacg atcgaaggtg
      181 taggaactgg gtacccttac gaagggaaac agatgtccga attagtgatc atcaagcctg
      241 cgggaaaacc ccttccattc tcctttgaca tactgtcatc agtctttcaa tatggaaacc
      301 gttgcttcac aaagtacccg gcagacatgc ctgactattt caagcaagca ttcccagatg
      361 gaatgtcata tgaaaggtca tttctatttg aggatggagc agttgctaca gccagctgga
      421 acattcgtct cgaaggaaat tgcttcatcc acaaatccat ctttcatggc gtaaactttc
      481 ccgctgatgg acccgtaatg aaaaagaaga caattgactg ggataagtcc ttcgaaaaaa
      541 tgactgtgtc taaagaggtg ctaagaggtg acgtgactat gtttcttatg ctcgaaggag
      601 gtggttctca cagatgccaa tttcactcca cttacaaaac agagaagccg gtcacactgc
      661 ccccgaatca tgtcgtagaa catcaaattg tgaggaccga ccttggccaa agtgcaaaag
      721 gctttacagt caagctggaa gcacatgccg cggctcatgt taaccctttg aaggttaaat
      781 aataatacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc
      841 cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac
      901 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      961 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1021 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct
     1081 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1141 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1201 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1261 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1321 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1381 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1441 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1501 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1561 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1621 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1681 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1741 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1801 taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa
     1861 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     1921 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg
     1981 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     2041 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     2101 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     2161 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     2221 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     2281 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     2341 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
     2401 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     2461 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     2521 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     2581 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     2641 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     2701 cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     2761 ataaaaatag gcgtatcacg aggcaga
//
