LOCUS       sequence-252119-        3869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP 
            cloning reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 87 to 121)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP49-2C(sfGFP)
FEATURES             Location/Qualifiers
     source          1..3869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        87..121
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             126..146
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             163..873
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    925..945
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      979..1073
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1309..2100
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     oriT            2246..2354
                     /note="incP origin of transfer"
     rep_origin      2390..2978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2879..2898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3132..3149
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3164..3304
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3230..3249)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3390..3412
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3406..3597)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3777..3863)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctctagc cggagtgaga cgggagttga cggctagctc agtcctaggt acagtgctag
      121 cgtacttgtt taactttaag aaggagatat acaatggtag cccgtaaagg cgaagagctg
      181 ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg tcataagttt
      241 tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct gaagttcatc
      301 tgtactactg gtaaactgcc ggtaccttgg ccgactctgg taacgacgct gacttatggt
      361 gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt caagtccgcc
      421 atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg cacgtacaaa
      481 acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga gctgaaaggc
      541 attgacttta aagaagatgg caatatcctg ggccataagc tggaatacaa ttttaacagc
      601 cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt
      661 cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca aaacactcca
      721 atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca aagcgttctg
      781 tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt aaccgcagcg
      841 ggcatcacgc atggtatgga tgaactgtac aaaggttcgt aacttctgac tggttgcacg
      901 ctcgtctctc gctttcgtga gacctactag tagcggccgc tgcagggagt tgtcttcgaa
      961 gacttcgctc tagtcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     1021 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccagggg
     1081 tccccaataa ttacgattta cgtatttaaa tgaaccttga ccgaacgcag cggtggtaac
     1141 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     1201 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     1261 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg
     1321 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     1381 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     1441 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     1501 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     1561 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     1621 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     1681 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     1741 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     1801 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg tgatgagcga
     1861 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     1921 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     1981 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     2041 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     2101 gacaattgtc cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg
     2161 tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc
     2221 cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg
     2281 ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc
     2341 tgcgaggctg gccgtaggcc ggccccgtag aaaagatcaa aggatcttct tgagatcctt
     2401 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2461 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2521 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2581 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2641 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2701 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2761 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2821 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2881 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2941 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3001 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3061 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     3181 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     3241 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     3301 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     3361 atccgcttac agacaagctg tgacagtctc cgggagctgc atgtgtcaga ggttttcacc
     3421 gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgcagcga
     3481 ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa
     3541 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     3601 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag
     3661 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg
     3721 taaacaactg gcggtatgga tgcggcgggg gcgcgcccag ctgtctaggg cggcggattt
     3781 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
     3841 gactgagcct ttcgttttat ttgatgcct
//
