LOCUS       sequence-252130-        2915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For course in ""Synthetic Biology: A Lab Manual"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: " 
            10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT     Sequence Label: tsPurple chromoprotein promoter-less
FEATURES             Location/Qualifiers
     source          1..2915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             23..34
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      747..818
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      834..861
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    868..888
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      889..946
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(970..987)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1141..1729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1221..1240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1912..2006)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2027..2686)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2601..2620
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2848..2866
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2870..2913)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agaaagagga gaaatactag atggcgagct tggttaagaa
       61 agatatgtgt gttaagatga cgatggaagg tactgtgaac ggttatcact ttaagtgcgt
      121 tggcgagggt gaaggcaagc cgttcgaggg cacgcagaac atgcgcattc gtgtcaccga
      181 gggcggtccg ctgccttttg cattcgacat cctggccccg tgctgtatgt acggctctaa
      241 gaccttcatt aaacacgtga gcggtatccc ggattacttt aaagagtcct ttccagaggg
      301 cttcacttgg gaacgtaccc agatttttga ggacggtggt gttctgaccg cgcaccaaga
      361 caccagcctg gaaggtaatt gcctgatcta taaagtgaag gttctgggta ccaatttccc
      421 ggcgaatggt ccggtgatgc aaaagaaaac cgcgggttgg gagccgtgcg tcgagatgct
      481 gtatccgcgt gacggcgtct tgtgtggtca gagcttgatg gcgctgaagt gcaccgatgg
      541 caatcatctg accagccacc tgcgcacgac gtatcgtagc cgtaaaccga gcaacgccgt
      601 taacatgccg gagttccatt ttggtgacca tcgcatcgaa atcctgaaag ctgagcaggg
      661 caaattctac gaacaatacg aatcggctgt cgcacgttac agcgatgtgc cggaaaaagc
      721 gacgtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      781 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      841 cttcgggtgg gcctttctgc gtttatatac tagtagcggc cgctgcagtc cggcaaaaaa
      901 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat
      961 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1021 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1081 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1141 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1201 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1261 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1321 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1381 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1441 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1501 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1561 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1621 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1681 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1741 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1801 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1861 atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc
     1921 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     1981 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     2041 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     2101 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     2161 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     2221 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     2281 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     2341 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     2401 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     2461 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     2521 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     2581 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     2641 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     2701 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     2761 gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt ctaagaaacc
     2821 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
     2881 aaaaaatcct tagctttcgc taaggatgat ttctg
//
