LOCUS       sequence-252140-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor template to insert 5xTet operator sequence into downstream of 
            HoxC cluster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Oksuz O, Narendra V, Lee CH, Descostes N, LeRoy G, Raviram R, 
            Blumenberg L, Karch K, Rocha PP, Garcia BA, Skok JA, Reinberg D
  TITLE     Capturing the Onset of PRC2-Mediated Repressive Domain Formation.
  JOURNAL   Mol Cell. 2018 Jun 21;70(6):1149-1162.e5. doi: 
            10.1016/j.molcel.2018.05.023.
  PUBMED    29932905
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.05"; journalName: "Mol Cell"; date: 
            "2018-06-21- 21"; volume: "70"; issue: "6"; pages: "1149-1162.e5"
COMMENT     Sequence Label: HoxC-downstream-5xTetO Donor
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    416..434
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    452..470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    488..506
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    524..542
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    560..578
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tgtggtggca cacacttgcc atctcagcaa tgggaacacg gagagagaag gatgctatgg
       61 ctaggtcact ggcaacctcc gaccagtgag atcctgtctc aaagagataa agccactaga
      121 agctgacctc tcacctccat atgcagatac actcacgtgc accatgcacc aacagatatg
      181 cacctgcaca tgtgcgaatg caaacgcatg cacagagggt ggacctacct ggttgagata
      241 ggtcacaagg gaaagatatt ggatgtttat acccagccac cggttcctct ttgcttcctg
      301 ctctgctgtg acatgaagga catctaccaa gcccctcctc ccacccctcc cactgtgaac
      361 taagctgatt caccaggctt tcctcaccat gatggattag accacgagtc aaaattccct
      421 atcagtgata gagaagttta ggcggactgt ttccctatca gtgatagaga acgtatctga
      481 gttttactcc ctatcagtga tagagattcc tgaaaatgct ccatccctat cagtgataga
      541 gattgatcat actggacgtt ccctatcagt gatagagatg agacctggtt gcccaggtgc
      601 ttaggttagt ccttatcctt tctccttcac cccaaagata ggtaactgca aatagtgaat
      661 cctgggtggg gtagtcatca ctgagctgta tcagttagca gtgattagca gttatcatat
      721 ctgcctcacc atagcctcta ccaccccaga gaacagacac aggttgggta ccagctcttc
      781 actcaccctt ccccagtctc ccaggattct cacttaggag ataacaggag ctcagaagca
      841 gcaagcctcg tggaggtccc atatccccca tttcagtctg cttggtcctc aagctctgga
      901 catggagaat tgctgaatac atgctttttg acccttaaga ccttggtgcc cctgggggaa
      961 gtcagcagga gctttaccat cttttcaatc ctggggtctg
//
