LOCUS       sequence-252162-        2800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BioBrick pSB1C3 plasmid that constitutively overexpresses 
            amilCP_Pink chromoprotein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: " 
            10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT     Sequence Label: amilCP_Pink chromoprotein
FEATURES             Location/Qualifiers
     source          1..2800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..24
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        25..59
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             68..79
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    755..775
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      776..833
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(857..874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1028..1616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1108..1127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1799..1893)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1914..2573)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2488..2507
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2735..2753
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2757..2800)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 tggaattcgc ggccgcttct agagtttacg gctagctcag tcctaggtac aatgctagct
       61 actagagaaa gaggagaaat actagatgag tgtgatcgct aaacaaatga cctacaaggt
      121 ttatatgtca ggcacggtca atggacacta ctttgaggtc gaaggcgatg gaaaaggtaa
      181 gccctacgag ggggagcaga cggtaaagct cactgtcacc aagggcggac ctctgccatt
      241 tgcttgggat attttatcac cacaggcctg ctacggaagc ataccattca ccaagtaccc
      301 tgaagacatc cctgactatg taaagcagtc attcccggag ggctatacat gggagaggat
      361 catgaacttt gaagatggtg cagtgtgtac tgtcagcaat gattccagca tccaaggcaa
      421 ctgtttcatc taccatgtca agttctctgg tttgaacttt cctcccaatg gacctgtcat
      481 gcagaagaag acacagggct gggaacccaa cactgagcgt ctctttgcac gagatggaat
      541 gctgctagga aacaacttta tggctctgaa gttagaagga ggcggtcact atttgtgtga
      601 atttaaaact acttacaagg caaagaagcc tgtgaagatg ccagggtatc actatgttga
      661 ccgcaaactg gatgtaacca atcacaacaa ggattacact tcggttgagc agtgtgaaat
      721 ttccattgca cgcaaacctg tggtcgccta ataatactag tagcggccgc tgcagtccgg
      781 caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc
      841 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      901 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      961 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1021 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1081 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1141 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1201 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1261 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1321 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1381 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1441 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1501 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1561 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1621 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1681 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1741 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat
     1801 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     1861 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     1921 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     1981 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     2041 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     2101 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     2161 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     2221 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     2281 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     2341 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     2401 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     2461 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     2521 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     2581 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     2641 ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta
     2701 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt
     2761 cagataaaaa aaatccttag ctttcgctaa ggatgatttc
//
