LOCUS       sequence-252164-        2757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For course in ""Synthetic Biology: A Lab Manual"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: " 
            10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT     Sequence Label: amilCP chromoprotein promoter-less
FEATURES             Location/Qualifiers
     source          1..2757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..44)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             69..80
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    756..776
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      777..834
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(858..875)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1029..1617)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1109..1128)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1800..1894)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1915..2574)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2489..2508
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2736..2754
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
       61 ttctagagaa agaggagaaa tactagatga gtgtgatcgc taaacaaatg acctacaagg
      121 tttatatgtc aggcacggtc aatggacact actttgaggt cgaaggcgat ggaaaaggta
      181 agccctacga gggggagcag acggtaaagc tcactgtcac caagggcgga cctctgccat
      241 ttgcttggga tattttatca ccacagtgtc agtacggaag cataccattc accaagtacc
      301 ctgaagacat ccctgactat gtaaagcagt cattcccgga gggctataca tgggagagga
      361 tcatgaactt tgaagatggt gcagtgtgta ctgtcagcaa tgattccagc atccaaggca
      421 actgtttcat ctaccatgtc aagttctctg gtttgaactt tcctcccaat ggacctgtca
      481 tgcagaagaa gacacagggc tgggaaccca acactgagcg tctctttgca cgagatggaa
      541 tgctgctagg aaacaacttt atggctctga agttagaagg aggcggtcac tatttgtgtg
      601 aatttaaaac tacttacaag gcaaagaagc ctgtgaagat gccagggtat cactatgttg
      661 accgcaaact ggatgtaacc aatcacaaca aggattacac ttcggttgag cagtgtgaaa
      721 tttccattgc acgcaaacct gtggtcgcct aataatacta gtagcggccg ctgcagtccg
      781 gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt
      841 cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      901 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      961 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1021 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1081 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1141 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1201 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1261 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1321 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1381 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1441 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1501 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1561 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1621 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1681 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1741 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga
     1801 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     1861 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     1921 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     1981 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     2041 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     2101 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     2161 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     2221 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     2281 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     2341 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     2401 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     2461 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     2521 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     2581 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     2641 attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct
     2701 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggcaga
//
