LOCUS       sequence-252168-         565 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Enhanced RNAi vector for C. elegans. Contains two T7 polymerase 
            termination sequences adjacent to the T7 promoter sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Sturm A, Saskoi E, Tibor K, Weinhardt N, Vellai T
  TITLE     Highly efficient RNAi and Cas9-based auto-cloning systems for C. 
            elegans research.
  JOURNAL   Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 
            10.1093/nar/gky516.
  PUBMED    29924347
REFERENCE   2  (bases 1 to 565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516."
COMMENT     Sequence Label: T444T
FEATURES             Location/Qualifiers
     source          1..565
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(176..197)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     terminator      complement(370..417)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     418..437
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        418..436
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     461..477
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     462..478
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(548..564)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(548..564)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
       61 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
      121 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
      181 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
      241 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
      301 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
      361 aaagccggcc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt atgctagtaa
      421 tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga cggtatcgat
      481 aagcttgata tcgaattcct gcagcccggg ggatccacgc gtcacgtggc tagccatgga
      541 accggtggat ccactagttc tagag
//
