LOCUS       sequence-252175-        2790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For course in ""Synthetic Biology: A Lab Manual"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: " 
            10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT     Sequence Label: asPink chromoprotein promoter-less
FEATURES             Location/Qualifiers
     source          1..2790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    709..729
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      730..787
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(811..828)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(982..1570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1062..1081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1753..1847)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1868..2527)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2442..2461
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2689..2707
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2711..2754)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2757..2778
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             2779..2790
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tactagatgg cgagcttcct gaaaaagacg atgccgttca aaaccacgat tgaaggcacg
       61 gtcaacggtc actactttaa atgtacgggt aaaggcgaag gtaacccgtt cgaaggcacc
      121 caggaaatga aaattgaagt catcgaaggc ggtccgctgc cgtttgcgtt tcatattctg
      181 agcacgtctt gcatgtacgg ctcaaagacc ttcatcaagt acgtgtcggg tatcccggat
      241 tactttaaac agagcttccc ggaaggcttt acctgggaac gtaccacgac ctatgaagat
      301 ggcggtttcc tgacggcgca ccaagacacc tctctggatg gtgactgtct ggtgtacaaa
      361 gttaagattc tgggcaacaa ttttccggcc gatggtccgg ttatgcagaa caaagcgggc
      421 cgttgggaac cggctacgga aatcgtctat gaagtggacg gcgttctgcg cggtcaatcc
      481 ctgatggccc tgaaatgccc gggcggtcgt catctgacct gtcatctgca cacgacctat
      541 cgtagcaaaa aaccggcgag cgccctgaaa atgccgggct ttcatttcga agatcaccgc
      601 attgaaatca tggaagaagt cgaaaaaggt aagtgctaca agcagtacga agcagcagtg
      661 ggtcgctatt gtgatgcggc accgagcaag ctgggtcata attaataata ctagtagcgg
      721 ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc
      781 gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
      841 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      901 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      961 aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa
     1021 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1081 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1141 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1201 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1261 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1321 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1381 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1441 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1501 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1561 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1621 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1681 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1741 ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     1801 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     1861 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     1921 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1981 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     2041 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     2101 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     2161 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     2221 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     2281 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     2341 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     2401 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     2461 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     2521 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     2581 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg
     2641 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2701 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
     2761 tcgcggccgc ttctagagaa agaggagaaa
//
