LOCUS       sequence-252177-        2824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime 
            chromoprotein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen
            P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen 
            A, Lundin ER, Wistrand-Yuen E, Forster AC
  TITLE     Engineering a palette of eukaryotic chromoproteins for bacterial 
            synthetic biology.
  JOURNAL   J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. 
            eCollection 2018.
  PUBMED    29760772
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2018-05-10"; volume: "12:8. doi"; pages: " 
            10.1186/s13036-018-0100-0. eCollection 2018"
COMMENT     Sequence Label: amajLime chromoprotein
FEATURES             Location/Qualifiers
     source          1..2824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..44)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        69..103
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             112..123
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    823..843
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      844..901
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(925..942)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1096..1684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1176..1195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1867..1961)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1982..2641)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2556..2575
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2803..2821
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
       61 ttctagagtt tacggctagc tcagtcctag gtacaatgct agctactaga gaaagaggag
      121 aaatactaga tggcactgag caacaagttt atcggtgatg atatgaaaat gacgtaccac
      181 atggacggct gcgtgaacgg ccactacttt acggtgaaag gtgaaggcaa cggtaaaccg
      241 tatgaaggca cccagacgag cacctttaaa gttacgatgg cgaatggcgg tccgctggcc
      301 tttagtttcg atattctgtc caccgtcttt aaatacggca accgttgctt cacggcgtat
      361 ccgaccagca tgccggatta ctttaagcaa gccttcccgg acggcatgtc ttatgaacgc
      421 acgtttacct acgaagatgg cggtgtggcg accgccagct gggaaatttc tctgaaaggc
      481 aactgtttcg aacataagag tacctttcac ggcgtgaatt tcccggcaga tggtccggtt
      541 atggctaaaa agaccacggg ttgggacccg tcatttgaaa aaatgacggt ctgcgatggc
      601 atcctgaagg gtgacgtgac cgcatttctg atgctgcaag gcggcggcaa ttatcgttgt
      661 caattccata cgtcctacaa aaccaaaaag ccggttacga tgccgccgaa ccatgtggtt
      721 gaacaccgta ttgcgcgcac cgatctggac aaaggtggca attcagttca gctgacggaa
      781 catgcagtcg ctcacatcac ctcggtcgtg ccgttctaat aatactagta gcggccgctg
      841 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
      901 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      961 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1021 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1081 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
     1141 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1201 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1261 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1321 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1381 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1441 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1501 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1561 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1621 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1681 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1741 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1801 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag
     1861 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     1921 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     1981 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2041 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2101 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2161 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2221 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2281 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2341 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2401 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     2461 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2521 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2581 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2641 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2701 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct
     2761 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2821 caga
//
